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Role of the purinergic and noradrenergic components in the potentiation by endothelin-1 of the sympathetic contraction of the rabbit central ear artery during cooling.
Br J Pharmacol. 1997 Sep; 122(1):172-8.BJ

Abstract

1. To examine the role of the purinergic and noradrenergic components in the potentiation of endothelin-1 on the vascular response to sympathetic nerve stimulation, we recorded the isometric response of isolated segments, 2 mm long, from the rabbit central ear artery to electrical field stimulation (1-8 Hz) under different conditions, at 37 degrees C during cooling (30 degrees C). 2. Electrical field stimulation produced frequency-dependent contraction, which was reduced during cooling (about 60% for 8 Hz). Both at 37 degrees C and 30 degrees C, phentolamine (1 microM) or blockade of alpha(1)-adrenoceptors with prazosin (1 microM) reduced, whereas blockade of alpha(2)-adrenoceptors with yohimbine (1 microM) increased, the contraction to electrical field stimulation. This contraction was increased after desensitization of P2-receptors with alpha, beta-methylene adenosine 5'-triphosphate (alpha, beta-meATP, 3 microM) at 37 degrees C but not at 30 degrees C, and was not modified by blockade of P2-receptors with pyridoxalphosphate-6-azophenyl-2,4'-disulphonic acid (PPADS, 30 microM) at either temperature. 3. Endothelin-1 (1, 3 and 10 nM) at 37 degrees C did not affect, but at 30 degrees C it potentiated in a concentration-dependent manner the contraction to electrical field stimulation (from 28 +/- 6 to 134 +/- 22%, for 8 Hz). At 37 degrees C, endothelin-1 in the presence of phentolamine or prazosin, but not in that of yohimbine, alpha, beta-meATP or PPADS, potentiated the contraction to electrical stimulation. At 30 degrees C, phentolamine or yohimbine reduced, prazosin did not modify and alpha, beta-meATP slightly increased the potentiation by endothelin-1 of the response to electrical stimulation. 4. The arterial contraction to ATP (2 mM) and the alpha(2)-adrenoceptor agonist BHT-920 (10 microM), but not that to (-)-noradrenaline (1 microM), was potentiated by endothelin-1 at both 37 degrees C and 30 degrees C. 5. These results in the rabbit central ear artery suggest that the sympathetic response: (a) at 37 degrees C, could be mediated mainly by activation of alpha(1)-adrenoceptors, with low participation of P2-receptors, (b) is diminished during cooling, probably by a reduction in the participation of alpha(1)-adrenoceptors, and in this condition the response could be mediated in part by P2-receptors, and (c) is potentiated by endothelin-1 during cooling, probably by increasing the response of both postjunctional alpha(2)-adrenoceptors and P2-receptors.

Authors+Show Affiliations

Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma, Madrid, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9298544

Citation

García-Villalón, A L., et al. "Role of the Purinergic and Noradrenergic Components in the Potentiation By Endothelin-1 of the Sympathetic Contraction of the Rabbit Central Ear Artery During Cooling." British Journal of Pharmacology, vol. 122, no. 1, 1997, pp. 172-8.
García-Villalón AL, Padilla J, Monge L, et al. Role of the purinergic and noradrenergic components in the potentiation by endothelin-1 of the sympathetic contraction of the rabbit central ear artery during cooling. Br J Pharmacol. 1997;122(1):172-8.
García-Villalón, A. L., Padilla, J., Monge, L., Fernández, N., Gómez, B., & Diéguez, G. (1997). Role of the purinergic and noradrenergic components in the potentiation by endothelin-1 of the sympathetic contraction of the rabbit central ear artery during cooling. British Journal of Pharmacology, 122(1), 172-8.
García-Villalón AL, et al. Role of the Purinergic and Noradrenergic Components in the Potentiation By Endothelin-1 of the Sympathetic Contraction of the Rabbit Central Ear Artery During Cooling. Br J Pharmacol. 1997;122(1):172-8. PubMed PMID: 9298544.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of the purinergic and noradrenergic components in the potentiation by endothelin-1 of the sympathetic contraction of the rabbit central ear artery during cooling. AU - García-Villalón,A L, AU - Padilla,J, AU - Monge,L, AU - Fernández,N, AU - Gómez,B, AU - Diéguez,G, PY - 1997/9/23/pubmed PY - 1997/9/23/medline PY - 1997/9/23/entrez SP - 172 EP - 8 JF - British journal of pharmacology JO - Br J Pharmacol VL - 122 IS - 1 N2 - 1. To examine the role of the purinergic and noradrenergic components in the potentiation of endothelin-1 on the vascular response to sympathetic nerve stimulation, we recorded the isometric response of isolated segments, 2 mm long, from the rabbit central ear artery to electrical field stimulation (1-8 Hz) under different conditions, at 37 degrees C during cooling (30 degrees C). 2. Electrical field stimulation produced frequency-dependent contraction, which was reduced during cooling (about 60% for 8 Hz). Both at 37 degrees C and 30 degrees C, phentolamine (1 microM) or blockade of alpha(1)-adrenoceptors with prazosin (1 microM) reduced, whereas blockade of alpha(2)-adrenoceptors with yohimbine (1 microM) increased, the contraction to electrical field stimulation. This contraction was increased after desensitization of P2-receptors with alpha, beta-methylene adenosine 5'-triphosphate (alpha, beta-meATP, 3 microM) at 37 degrees C but not at 30 degrees C, and was not modified by blockade of P2-receptors with pyridoxalphosphate-6-azophenyl-2,4'-disulphonic acid (PPADS, 30 microM) at either temperature. 3. Endothelin-1 (1, 3 and 10 nM) at 37 degrees C did not affect, but at 30 degrees C it potentiated in a concentration-dependent manner the contraction to electrical field stimulation (from 28 +/- 6 to 134 +/- 22%, for 8 Hz). At 37 degrees C, endothelin-1 in the presence of phentolamine or prazosin, but not in that of yohimbine, alpha, beta-meATP or PPADS, potentiated the contraction to electrical stimulation. At 30 degrees C, phentolamine or yohimbine reduced, prazosin did not modify and alpha, beta-meATP slightly increased the potentiation by endothelin-1 of the response to electrical stimulation. 4. The arterial contraction to ATP (2 mM) and the alpha(2)-adrenoceptor agonist BHT-920 (10 microM), but not that to (-)-noradrenaline (1 microM), was potentiated by endothelin-1 at both 37 degrees C and 30 degrees C. 5. These results in the rabbit central ear artery suggest that the sympathetic response: (a) at 37 degrees C, could be mediated mainly by activation of alpha(1)-adrenoceptors, with low participation of P2-receptors, (b) is diminished during cooling, probably by a reduction in the participation of alpha(1)-adrenoceptors, and in this condition the response could be mediated in part by P2-receptors, and (c) is potentiated by endothelin-1 during cooling, probably by increasing the response of both postjunctional alpha(2)-adrenoceptors and P2-receptors. SN - 0007-1188 UR - https://www.unboundmedicine.com/medline/citation/9298544/Role_of_the_purinergic_and_noradrenergic_components_in_the_potentiation_by_endothelin_1_of_the_sympathetic_contraction_of_the_rabbit_central_ear_artery_during_cooling_ L2 - https://doi.org/10.1038/sj.bjp.0701359 DB - PRIME DP - Unbound Medicine ER -