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Expression of different levels of enzymes from the Pichia stipitis XYL1 and XYL2 genes in Saccharomyces cerevisiae and its effects on product formation during xylose utilisation.
Appl Microbiol Biotechnol. 1997 Aug; 48(2):218-24.AM

Abstract

Saccharomyces cerevisiae was transformed with the Pichia stipitis CBS 6054 XYL1 and XYL2 genes encoding xylose reductase (XR) and xylitol dehydrogenase (XDH) respectively. The XYL1 and XYL2 genes were placed under the control of the alcohol dehydrogenase 1 (ADH1) and phosphoglycerate kinase (PGK1) promoters in the yeast vector YEp24. Different vector constructions were made resulting in different specific activities of XR and XDH. The XR:XDH ratio (ratio of specific enzyme activities) of the transformed S. cerevisiae strains varied from 17.5 to 0.06. In order to enhance xylose utilisation in the XYL1-, XYL2-containing S. cerevisiae strains, the native genes encoding transketolase and transaldolase were also overexpressed. A strain with an XR:XDH ratio of 17.5 formed 0.82 g xylitol/g consumed xylose, whereas a strain with an XR:XDH ratio of 5.0 formed 0.58 g xylitol/g xylose. The strain with an XR:XDH ratio of 0.06, on the other hand, formed no xylitol and less glycerol and acetic acid compared with strains with the higher XR:XDH ratios. In addition, the strain with an XR:XDH ratio of 0.06 produced more ethanol than the other strains.

Authors+Show Affiliations

Department of Applied Microbiology, Lund Institute of Technology/Lund University, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9299780

Citation

Walfridsson, M, et al. "Expression of Different Levels of Enzymes From the Pichia Stipitis XYL1 and XYL2 Genes in Saccharomyces Cerevisiae and Its Effects On Product Formation During Xylose Utilisation." Applied Microbiology and Biotechnology, vol. 48, no. 2, 1997, pp. 218-24.
Walfridsson M, Anderlund M, Bao X, et al. Expression of different levels of enzymes from the Pichia stipitis XYL1 and XYL2 genes in Saccharomyces cerevisiae and its effects on product formation during xylose utilisation. Appl Microbiol Biotechnol. 1997;48(2):218-24.
Walfridsson, M., Anderlund, M., Bao, X., & Hahn-Hägerdal, B. (1997). Expression of different levels of enzymes from the Pichia stipitis XYL1 and XYL2 genes in Saccharomyces cerevisiae and its effects on product formation during xylose utilisation. Applied Microbiology and Biotechnology, 48(2), 218-24.
Walfridsson M, et al. Expression of Different Levels of Enzymes From the Pichia Stipitis XYL1 and XYL2 Genes in Saccharomyces Cerevisiae and Its Effects On Product Formation During Xylose Utilisation. Appl Microbiol Biotechnol. 1997;48(2):218-24. PubMed PMID: 9299780.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of different levels of enzymes from the Pichia stipitis XYL1 and XYL2 genes in Saccharomyces cerevisiae and its effects on product formation during xylose utilisation. AU - Walfridsson,M, AU - Anderlund,M, AU - Bao,X, AU - Hahn-Hägerdal,B, PY - 1997/8/1/pubmed PY - 1997/9/23/medline PY - 1997/8/1/entrez SP - 218 EP - 24 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 48 IS - 2 N2 - Saccharomyces cerevisiae was transformed with the Pichia stipitis CBS 6054 XYL1 and XYL2 genes encoding xylose reductase (XR) and xylitol dehydrogenase (XDH) respectively. The XYL1 and XYL2 genes were placed under the control of the alcohol dehydrogenase 1 (ADH1) and phosphoglycerate kinase (PGK1) promoters in the yeast vector YEp24. Different vector constructions were made resulting in different specific activities of XR and XDH. The XR:XDH ratio (ratio of specific enzyme activities) of the transformed S. cerevisiae strains varied from 17.5 to 0.06. In order to enhance xylose utilisation in the XYL1-, XYL2-containing S. cerevisiae strains, the native genes encoding transketolase and transaldolase were also overexpressed. A strain with an XR:XDH ratio of 17.5 formed 0.82 g xylitol/g consumed xylose, whereas a strain with an XR:XDH ratio of 5.0 formed 0.58 g xylitol/g xylose. The strain with an XR:XDH ratio of 0.06, on the other hand, formed no xylitol and less glycerol and acetic acid compared with strains with the higher XR:XDH ratios. In addition, the strain with an XR:XDH ratio of 0.06 produced more ethanol than the other strains. SN - 0175-7598 UR - https://www.unboundmedicine.com/medline/citation/9299780/Expression_of_different_levels_of_enzymes_from_the_Pichia_stipitis_XYL1_and_XYL2_genes_in_Saccharomyces_cerevisiae_and_its_effects_on_product_formation_during_xylose_utilisation_ L2 - https://dx.doi.org/10.1007/s002530051041 DB - PRIME DP - Unbound Medicine ER -