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The SecDFyajC domain of preprotein translocase controls preprotein movement by regulating SecA membrane cycling.
EMBO J 1997; 16(16):4871-9EJ

Abstract

Escherichia coli preprotein translocase comprises a membrane-embedded hexameric complex of SecY, SecE, SecG, SecD, SecF and YajC (SecYEGDFyajC) and the peripheral ATPase SecA. The energy of ATP binding and hydrolysis promotes cycles of membrane insertion and deinsertion of SecA and catalyzes the movement of the preprotein across the membrane. The proton motive force (PMF), though not essential, greatly accelerates late stages of translocation. We now report that the SecDFyajC domain of translocase slows the movement of preprotein in transit against both reverse and forward translocation and exerts this control through stabilization of the inserted form of SecA. This mechanism allows the accumulation of specific translocation intermediates which can then complete translocation under the driving force of the PMF. These findings establish a functional relationship between SecA membrane insertion and preprotein translocation and show that SecDFyajC controls SecA membrane cycling to regulate the movement of the translocating preprotein.

Authors+Show Affiliations

Dartmouth Medical School, Department of Biochemistry, Hanover, NH 03755, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9305629

Citation

Duong, F, and W Wickner. "The SecDFyajC Domain of Preprotein Translocase Controls Preprotein Movement By Regulating SecA Membrane Cycling." The EMBO Journal, vol. 16, no. 16, 1997, pp. 4871-9.
Duong F, Wickner W. The SecDFyajC domain of preprotein translocase controls preprotein movement by regulating SecA membrane cycling. EMBO J. 1997;16(16):4871-9.
Duong, F., & Wickner, W. (1997). The SecDFyajC domain of preprotein translocase controls preprotein movement by regulating SecA membrane cycling. The EMBO Journal, 16(16), pp. 4871-9.
Duong F, Wickner W. The SecDFyajC Domain of Preprotein Translocase Controls Preprotein Movement By Regulating SecA Membrane Cycling. EMBO J. 1997 Aug 15;16(16):4871-9. PubMed PMID: 9305629.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The SecDFyajC domain of preprotein translocase controls preprotein movement by regulating SecA membrane cycling. AU - Duong,F, AU - Wickner,W, PY - 1997/8/15/pubmed PY - 1997/9/26/medline PY - 1997/8/15/entrez SP - 4871 EP - 9 JF - The EMBO journal JO - EMBO J. VL - 16 IS - 16 N2 - Escherichia coli preprotein translocase comprises a membrane-embedded hexameric complex of SecY, SecE, SecG, SecD, SecF and YajC (SecYEGDFyajC) and the peripheral ATPase SecA. The energy of ATP binding and hydrolysis promotes cycles of membrane insertion and deinsertion of SecA and catalyzes the movement of the preprotein across the membrane. The proton motive force (PMF), though not essential, greatly accelerates late stages of translocation. We now report that the SecDFyajC domain of translocase slows the movement of preprotein in transit against both reverse and forward translocation and exerts this control through stabilization of the inserted form of SecA. This mechanism allows the accumulation of specific translocation intermediates which can then complete translocation under the driving force of the PMF. These findings establish a functional relationship between SecA membrane insertion and preprotein translocation and show that SecDFyajC controls SecA membrane cycling to regulate the movement of the translocating preprotein. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/9305629/The_SecDFyajC_domain_of_preprotein_translocase_controls_preprotein_movement_by_regulating_SecA_membrane_cycling_ L2 - http://emboj.embopress.org/cgi/pmidlookup?view=long&pmid=9305629 DB - PRIME DP - Unbound Medicine ER -