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Fgfr2 and osteopontin domains in the developing skull vault are mutually exclusive and can be altered by locally applied FGF2.
Development. 1997 Sep; 124(17):3375-84.D

Abstract

Mutations in the human fibroblast growth factor receptor type 2 (FGFR2) gene cause craniosynostosis, particularly affecting the coronal suture. We show here that, in the fetal mouse skull vault, Fgfr2 transcripts are most abundant at the periphery of the membrane bones; they are mutually exclusive with those of osteopontin (an early marker of osteogenic differentiation) but coincide with sites of rapid cell proliferation. Fibroblast growth factor type 2 (FGF2) protein, which has a high affinity for the FGFR2 splice variant associated with craniosynostosis, is locally abundant; immunohistochemical detection showed it to be present at low levels in Fgfr2 expression domains and at high levels in differentiated areas. Implantation of FGF2-soaked beads onto the fetal coronal suture by ex utero surgery resulted in ectopic osteopontin expression, encircled by Fgfr2 expression, after 48 hours. We suggest that increased FGF/FGFR signalling in the developing skull, whether due to FGFR2 mutation or to ectopic FGF2, shifts the cell proliferation/differentiation balance towards differentiation by enhancing the normal paracrine down-regulation of Fgfr2.

Authors+Show Affiliations

Department of Human Anatomy, Oxford, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9310332

Citation

Iseki, S, et al. "Fgfr2 and Osteopontin Domains in the Developing Skull Vault Are Mutually Exclusive and Can Be Altered By Locally Applied FGF2." Development (Cambridge, England), vol. 124, no. 17, 1997, pp. 3375-84.
Iseki S, Wilkie AO, Heath JK, et al. Fgfr2 and osteopontin domains in the developing skull vault are mutually exclusive and can be altered by locally applied FGF2. Development. 1997;124(17):3375-84.
Iseki, S., Wilkie, A. O., Heath, J. K., Ishimaru, T., Eto, K., & Morriss-Kay, G. M. (1997). Fgfr2 and osteopontin domains in the developing skull vault are mutually exclusive and can be altered by locally applied FGF2. Development (Cambridge, England), 124(17), 3375-84.
Iseki S, et al. Fgfr2 and Osteopontin Domains in the Developing Skull Vault Are Mutually Exclusive and Can Be Altered By Locally Applied FGF2. Development. 1997;124(17):3375-84. PubMed PMID: 9310332.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fgfr2 and osteopontin domains in the developing skull vault are mutually exclusive and can be altered by locally applied FGF2. AU - Iseki,S, AU - Wilkie,A O, AU - Heath,J K, AU - Ishimaru,T, AU - Eto,K, AU - Morriss-Kay,G M, PY - 1997/10/6/pubmed PY - 1997/10/6/medline PY - 1997/10/6/entrez SP - 3375 EP - 84 JF - Development (Cambridge, England) JO - Development VL - 124 IS - 17 N2 - Mutations in the human fibroblast growth factor receptor type 2 (FGFR2) gene cause craniosynostosis, particularly affecting the coronal suture. We show here that, in the fetal mouse skull vault, Fgfr2 transcripts are most abundant at the periphery of the membrane bones; they are mutually exclusive with those of osteopontin (an early marker of osteogenic differentiation) but coincide with sites of rapid cell proliferation. Fibroblast growth factor type 2 (FGF2) protein, which has a high affinity for the FGFR2 splice variant associated with craniosynostosis, is locally abundant; immunohistochemical detection showed it to be present at low levels in Fgfr2 expression domains and at high levels in differentiated areas. Implantation of FGF2-soaked beads onto the fetal coronal suture by ex utero surgery resulted in ectopic osteopontin expression, encircled by Fgfr2 expression, after 48 hours. We suggest that increased FGF/FGFR signalling in the developing skull, whether due to FGFR2 mutation or to ectopic FGF2, shifts the cell proliferation/differentiation balance towards differentiation by enhancing the normal paracrine down-regulation of Fgfr2. SN - 0950-1991 UR - https://www.unboundmedicine.com/medline/citation/9310332/Fgfr2_and_osteopontin_domains_in_the_developing_skull_vault_are_mutually_exclusive_and_can_be_altered_by_locally_applied_FGF2_ L2 - http://dev.biologists.org/cgi/pmidlookup?view=long&pmid=9310332 DB - PRIME DP - Unbound Medicine ER -