Tags

Type your tag names separated by a space and hit enter

Gliadin specific, HLA DQ2-restricted T cells are commonly found in small intestinal biopsies from coeliac disease patients, but not from controls.
Scand J Immunol. 1997 Sep; 46(3):103-9.SJ

Abstract

The authors have analysed gliadin specific, CD4+ T cells isolated from small intestinal biopsies of 23 adult coeliac disease patients (20 on a gluten-free diet and three untreated) and nine control patients. The biopsies were stimulated ex vivo with a peptic/tryptic digest of gliadin for 24 h, and activated T cells were positively selected with paramagnetic beads coated with an antibody against the interleukin-2 receptor. The T cells were expanded and tested for gliadin reactivity and HLA restriction. Gliadin specific, polyclonal T cell lines were recovered from biopsies of all 23 patients. Inhibition studies of T cell lines from 21 patients with anti-HLA monoclonal antibodies indicated predominant presentation of the gliadin antigen by HLA-DQ2 in T cell lines from 11 patients (lines from seven patients with complete MoAb inhibition, the remaining with incomplete inhibition) and incomplete inhibition by HLA-DR3 in lines from three patients. Nine gliadin specific T cell clones from six patients were established; all of these were HLA-DQ2 restricted. Gliadin specific T cells were not found in biopsies from the non-coeliac controls. Our findings demonstrate that gliadin reactive T cells are commonly found in the intestinal mucosa of CD patients and they support the notion that the majority of T cell recognize gliadin peptide(s) when presented by the disease associated DQ2 molecules.

Authors+Show Affiliations

Institute of Transplantation Immunology, National Hospital, Oslo, Norway.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Corrected and Republished Article
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9315123

Citation

Molberg, O, et al. "Gliadin Specific, HLA DQ2-restricted T Cells Are Commonly Found in Small Intestinal Biopsies From Coeliac Disease Patients, but Not From Controls." Scandinavian Journal of Immunology, vol. 46, no. 3, 1997, pp. 103-9.
Molberg O, Kett K, Scott H, et al. Gliadin specific, HLA DQ2-restricted T cells are commonly found in small intestinal biopsies from coeliac disease patients, but not from controls. Scand J Immunol. 1997;46(3):103-9.
Molberg, O., Kett, K., Scott, H., Thorsby, E., Sollid, L. M., & Lundin, K. E. (1997). Gliadin specific, HLA DQ2-restricted T cells are commonly found in small intestinal biopsies from coeliac disease patients, but not from controls. Scandinavian Journal of Immunology, 46(3), 103-9.
Molberg O, et al. Gliadin Specific, HLA DQ2-restricted T Cells Are Commonly Found in Small Intestinal Biopsies From Coeliac Disease Patients, but Not From Controls. Scand J Immunol. 1997;46(3):103-9. PubMed PMID: 9315123.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gliadin specific, HLA DQ2-restricted T cells are commonly found in small intestinal biopsies from coeliac disease patients, but not from controls. AU - Molberg,O, AU - Kett,K, AU - Scott,H, AU - Thorsby,E, AU - Sollid,L M, AU - Lundin,K E, PY - 1997/10/7/pubmed PY - 1997/10/7/medline PY - 1997/10/7/entrez SP - 103 EP - 9 JF - Scandinavian journal of immunology JO - Scand J Immunol VL - 46 IS - 3 N2 - The authors have analysed gliadin specific, CD4+ T cells isolated from small intestinal biopsies of 23 adult coeliac disease patients (20 on a gluten-free diet and three untreated) and nine control patients. The biopsies were stimulated ex vivo with a peptic/tryptic digest of gliadin for 24 h, and activated T cells were positively selected with paramagnetic beads coated with an antibody against the interleukin-2 receptor. The T cells were expanded and tested for gliadin reactivity and HLA restriction. Gliadin specific, polyclonal T cell lines were recovered from biopsies of all 23 patients. Inhibition studies of T cell lines from 21 patients with anti-HLA monoclonal antibodies indicated predominant presentation of the gliadin antigen by HLA-DQ2 in T cell lines from 11 patients (lines from seven patients with complete MoAb inhibition, the remaining with incomplete inhibition) and incomplete inhibition by HLA-DR3 in lines from three patients. Nine gliadin specific T cell clones from six patients were established; all of these were HLA-DQ2 restricted. Gliadin specific T cells were not found in biopsies from the non-coeliac controls. Our findings demonstrate that gliadin reactive T cells are commonly found in the intestinal mucosa of CD patients and they support the notion that the majority of T cell recognize gliadin peptide(s) when presented by the disease associated DQ2 molecules. SN - 0300-9475 UR - https://www.unboundmedicine.com/medline/citation/9315123/Gliadin_specific_HLA_DQ2_restricted_T_cells_are_commonly_found_in_small_intestinal_biopsies_from_coeliac_disease_patients_but_not_from_controls_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0300-9475&date=1997&volume=46&issue=3&spage=103 DB - PRIME DP - Unbound Medicine ER -