Tags

Type your tag names separated by a space and hit enter

Excision of a conjugative transposon in vitro by the Int and Xis proteins of Tn916.
Nucleic Acids Res. 1997 Oct 15; 25(20):4061-6.NA

Abstract

The roles of purified Int and Xis proteins of the conjugative transposon Tn 916 in excision of a deletion derivative of the closely related element Tn 1545 were investigated. At a low salt concentration (37.5 mM NaCl), Int alone was able to promote limited excision to produce a covalently closed circular form of the transposon, showing that Tn 916 Int can catalyze both DNA cleavage and strand exchange. This reaction was stimulated by Xis. At higher salt concentrations (150 mM NaCl), excision by Int alone was reduced to barely detectable levels and Xis was required for excision. The low salt, Xis-stimulated reaction was approximately 8-fold more efficient than the high salt, Xis-dependent reaction. These results reflect in vivo requirements for Int and Xis in excision.

Authors+Show Affiliations

Department of Microbiology and Immunology, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9321658

Citation

Rudy, C, et al. "Excision of a Conjugative Transposon in Vitro By the Int and Xis Proteins of Tn916." Nucleic Acids Research, vol. 25, no. 20, 1997, pp. 4061-6.
Rudy C, Taylor KL, Hinerfeld D, et al. Excision of a conjugative transposon in vitro by the Int and Xis proteins of Tn916. Nucleic Acids Res. 1997;25(20):4061-6.
Rudy, C., Taylor, K. L., Hinerfeld, D., Scott, J. R., & Churchward, G. (1997). Excision of a conjugative transposon in vitro by the Int and Xis proteins of Tn916. Nucleic Acids Research, 25(20), 4061-6.
Rudy C, et al. Excision of a Conjugative Transposon in Vitro By the Int and Xis Proteins of Tn916. Nucleic Acids Res. 1997 Oct 15;25(20):4061-6. PubMed PMID: 9321658.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Excision of a conjugative transposon in vitro by the Int and Xis proteins of Tn916. AU - Rudy,C, AU - Taylor,K L, AU - Hinerfeld,D, AU - Scott,J R, AU - Churchward,G, PY - 1997/10/10/pubmed PY - 1997/10/10/medline PY - 1997/10/10/entrez SP - 4061 EP - 6 JF - Nucleic acids research JO - Nucleic Acids Res. VL - 25 IS - 20 N2 - The roles of purified Int and Xis proteins of the conjugative transposon Tn 916 in excision of a deletion derivative of the closely related element Tn 1545 were investigated. At a low salt concentration (37.5 mM NaCl), Int alone was able to promote limited excision to produce a covalently closed circular form of the transposon, showing that Tn 916 Int can catalyze both DNA cleavage and strand exchange. This reaction was stimulated by Xis. At higher salt concentrations (150 mM NaCl), excision by Int alone was reduced to barely detectable levels and Xis was required for excision. The low salt, Xis-stimulated reaction was approximately 8-fold more efficient than the high salt, Xis-dependent reaction. These results reflect in vivo requirements for Int and Xis in excision. SN - 0305-1048 UR - https://www.unboundmedicine.com/medline/citation/9321658/Excision_of_a_conjugative_transposon_in_vitro_by_the_Int_and_Xis_proteins_of_Tn916_ L2 - https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/25.20.4061 DB - PRIME DP - Unbound Medicine ER -