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Properties of primary murine stroma induced by macrophage colony-stimulating factor.
J Cell Physiol. 1997 Oct; 173(1):1-9.JC

Abstract

The ability of purified human macrophage colony-stimulating factor (M-CSF) to accelerate the formation of stromal cells from murine bone marrow cells was investigated. The liquid culture of the marrow cells with M-CSF resulted in the formation of monolayers of macrophages on day 7. When the M-CSF was removed on that day and the residual adherent cells were cultured in the absence of M-CSF for an additional 7 days, many colonies appeared with cells that were morphologically distinguishable from M-CSF-derived macrophages. The appearance of the colonies was dependent on the concentration of M-CSF used at the beginning of the culture. Each colony was isolated as a single clone and analyzed. All clones were negative for esterase staining. These cells did not express M-CSF receptor mRNA and did not show a mitogenic response to M-CSF. On the contrary, these cells could be stimulated to proliferate by fibroblast growth factor and platelet-derived growth factor. The polymerase chain reaction analysis of these cells demonstrated constitutive expression of mRNA for M-CSF, stem cell factor, and interleukin (IL)-1, but not IL-3. Some clones expressed mRNA for granulocyte/M-CSF and IL-6. We also examined the ability of the cells to maintain murine bone marrow high proliferative potential colony-forming cells (HPP-CFC) in a coculture system. Most of the clones showed a significant increase in total HPP-CFC numbers after 2 weeks of coculture, although the extent of stimulation differed among clones. These results suggested that the colonies established by M-CSF were composed of functional stromal cells that were phenotypically different from macrophages.

Authors+Show Affiliations

Biochemical Research Laboratory, Morinaga Milk Industry Co. Ltd., Zama, Kanagawa, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9326443

Citation

Yamada, M, et al. "Properties of Primary Murine Stroma Induced By Macrophage Colony-stimulating Factor." Journal of Cellular Physiology, vol. 173, no. 1, 1997, pp. 1-9.
Yamada M, Suzu S, Akaiwa E, et al. Properties of primary murine stroma induced by macrophage colony-stimulating factor. J Cell Physiol. 1997;173(1):1-9.
Yamada, M., Suzu, S., Akaiwa, E., Wakimoto, N., Hatake, K., Motoyoshi, K., & Shimamura, S. (1997). Properties of primary murine stroma induced by macrophage colony-stimulating factor. Journal of Cellular Physiology, 173(1), 1-9.
Yamada M, et al. Properties of Primary Murine Stroma Induced By Macrophage Colony-stimulating Factor. J Cell Physiol. 1997;173(1):1-9. PubMed PMID: 9326443.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Properties of primary murine stroma induced by macrophage colony-stimulating factor. AU - Yamada,M, AU - Suzu,S, AU - Akaiwa,E, AU - Wakimoto,N, AU - Hatake,K, AU - Motoyoshi,K, AU - Shimamura,S, PY - 1997/10/27/pubmed PY - 2000/6/20/medline PY - 1997/10/27/entrez SP - 1 EP - 9 JF - Journal of cellular physiology JO - J Cell Physiol VL - 173 IS - 1 N2 - The ability of purified human macrophage colony-stimulating factor (M-CSF) to accelerate the formation of stromal cells from murine bone marrow cells was investigated. The liquid culture of the marrow cells with M-CSF resulted in the formation of monolayers of macrophages on day 7. When the M-CSF was removed on that day and the residual adherent cells were cultured in the absence of M-CSF for an additional 7 days, many colonies appeared with cells that were morphologically distinguishable from M-CSF-derived macrophages. The appearance of the colonies was dependent on the concentration of M-CSF used at the beginning of the culture. Each colony was isolated as a single clone and analyzed. All clones were negative for esterase staining. These cells did not express M-CSF receptor mRNA and did not show a mitogenic response to M-CSF. On the contrary, these cells could be stimulated to proliferate by fibroblast growth factor and platelet-derived growth factor. The polymerase chain reaction analysis of these cells demonstrated constitutive expression of mRNA for M-CSF, stem cell factor, and interleukin (IL)-1, but not IL-3. Some clones expressed mRNA for granulocyte/M-CSF and IL-6. We also examined the ability of the cells to maintain murine bone marrow high proliferative potential colony-forming cells (HPP-CFC) in a coculture system. Most of the clones showed a significant increase in total HPP-CFC numbers after 2 weeks of coculture, although the extent of stimulation differed among clones. These results suggested that the colonies established by M-CSF were composed of functional stromal cells that were phenotypically different from macrophages. SN - 0021-9541 UR - https://www.unboundmedicine.com/medline/citation/9326443/Properties_of_primary_murine_stroma_induced_by_macrophage_colony_stimulating_factor_ DB - PRIME DP - Unbound Medicine ER -