Tags

Type your tag names separated by a space and hit enter

Characterization of oligosaccharide composition and structure by quadrupole ion trap mass spectrometry.
Rapid Commun Mass Spectrom. 1997; 11(14):1493-504.RC

Abstract

The use of electrospray ionization-quadrupole ion trap mass spectrometry for the characterization of linear oligosaccharides and N-linked protein oligosaccharide mixtures is described. Tandem mass spectrometry (MS/MS) experiments with orders higher than two offer a number of ways to enhance MS/MS spectra and to derive information not present in MS and MS2 spectra. Three such methods are presented in this paper. (a) Collisional activation of permethylated oligosaccharide molecular ions (MS2) as illustrated by maltoheptaose, produces abundant fragments from glycosidic bond cleavages which indicate composition and sequence, and weak cross-ring cleavage products which denote specific linkages within the oligosaccharide. Through the trapping and further dissociation of these fragments (MSn), cross-ring cleavage products can be confirmed and their relative abundances increased to facilitate interpretation. (b) The mechanisms of formation of two isobaric ions or ions isobaric with another ion's isotope peaks, such as those present in the MS2 spectrum of the ribonuclease B oligosaccharide GlcNAc2-Man5 can be independently established by separate MS3 experiments. (c) Ions in the MS2 spectrum, specific for individual components of an isobaric mixture, can be isolated and characterized by further stages of fragmentation. This is illustrated by two isobaric oligosaccharides from chicken ovalbumin of the composition HexNAc5Hex5. These findings indicate the utility of ion trap mass spectrometry towards the facile determination of oligosaccharide composition, sequence, branching and linkage, providing a wealth of structural information not obtainable by other individual methods of carbohydrate mass spectrometric analysis.

Authors+Show Affiliations

Department of Chemistry, Northeastern University, Boston, Massachusetts 02115, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9332019

Citation

Weiskopf, A S., et al. "Characterization of Oligosaccharide Composition and Structure By Quadrupole Ion Trap Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 11, no. 14, 1997, pp. 1493-504.
Weiskopf AS, Vouros P, Harvey DJ. Characterization of oligosaccharide composition and structure by quadrupole ion trap mass spectrometry. Rapid Commun Mass Spectrom. 1997;11(14):1493-504.
Weiskopf, A. S., Vouros, P., & Harvey, D. J. (1997). Characterization of oligosaccharide composition and structure by quadrupole ion trap mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 11(14), 1493-504.
Weiskopf AS, Vouros P, Harvey DJ. Characterization of Oligosaccharide Composition and Structure By Quadrupole Ion Trap Mass Spectrometry. Rapid Commun Mass Spectrom. 1997;11(14):1493-504. PubMed PMID: 9332019.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of oligosaccharide composition and structure by quadrupole ion trap mass spectrometry. AU - Weiskopf,A S, AU - Vouros,P, AU - Harvey,D J, PY - 1997/1/1/pubmed PY - 1997/10/23/medline PY - 1997/1/1/entrez SP - 1493 EP - 504 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 11 IS - 14 N2 - The use of electrospray ionization-quadrupole ion trap mass spectrometry for the characterization of linear oligosaccharides and N-linked protein oligosaccharide mixtures is described. Tandem mass spectrometry (MS/MS) experiments with orders higher than two offer a number of ways to enhance MS/MS spectra and to derive information not present in MS and MS2 spectra. Three such methods are presented in this paper. (a) Collisional activation of permethylated oligosaccharide molecular ions (MS2) as illustrated by maltoheptaose, produces abundant fragments from glycosidic bond cleavages which indicate composition and sequence, and weak cross-ring cleavage products which denote specific linkages within the oligosaccharide. Through the trapping and further dissociation of these fragments (MSn), cross-ring cleavage products can be confirmed and their relative abundances increased to facilitate interpretation. (b) The mechanisms of formation of two isobaric ions or ions isobaric with another ion's isotope peaks, such as those present in the MS2 spectrum of the ribonuclease B oligosaccharide GlcNAc2-Man5 can be independently established by separate MS3 experiments. (c) Ions in the MS2 spectrum, specific for individual components of an isobaric mixture, can be isolated and characterized by further stages of fragmentation. This is illustrated by two isobaric oligosaccharides from chicken ovalbumin of the composition HexNAc5Hex5. These findings indicate the utility of ion trap mass spectrometry towards the facile determination of oligosaccharide composition, sequence, branching and linkage, providing a wealth of structural information not obtainable by other individual methods of carbohydrate mass spectrometric analysis. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/9332019/Characterization_of_oligosaccharide_composition_and_structure_by_quadrupole_ion_trap_mass_spectrometry_ L2 - https://doi.org/10.1002/(SICI)1097-0231(199709)11:14<1493::AID-RCM40>3.0.CO;2-1 DB - PRIME DP - Unbound Medicine ER -