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Activation of ATP P2X receptors elicits glutamate release from sensory neuron synapses.
Nature. 1997 Oct 16; 389(6652):749-53.Nat

Abstract

Painful stimuli to the skin initiate action potentials in the peripheral terminals of dorsal root ganglion (DRG) neurons. These action potentials propagate to DRG central terminals in the dorsal horn of the spinal cord, evoking release of excitatory transmitters such as glutamate onto postsynaptic dorsal horn neurons. P2X receptors, a family of ligand-gated ion channels activated by the endogenous ligand ATP, are highly expressed by DRG neurons. Immunoreactivity to P2X receptors has been identified in the dorsal horn superficial laminae associated with nociceptive DRG central terminals, suggesting the presence of presynaptic P2X receptors. Here we have used a DRG-dorsal horn co-culture system to show that P2X receptors are localized at presynaptic sites on DRG neurons; that activation of these receptors results in increased frequency of spontaneous glutamate release; and that activation of P2X receptors at or near presynaptic DRG nerve terminals elicits action potentials that cause evoked glutamate release. Thus activation of P2X receptors at DRG central terminals can modify sensory signal throughput, and might even initiate sensory signals at central synapses without direct peripheral input. This putative central modulation and generation of sensory signals may be associated with physiological and pathological pain sensation, making presynaptic P2X receptors a possible target for pain therapy.

Authors+Show Affiliations

Department of Physiology and Cellular Biophysics, Columbia University, New York, New York 10032, USA. jg147@columbia.eduNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9338789

Citation

Gu, J G., and A B. MacDermott. "Activation of ATP P2X Receptors Elicits Glutamate Release From Sensory Neuron Synapses." Nature, vol. 389, no. 6652, 1997, pp. 749-53.
Gu JG, MacDermott AB. Activation of ATP P2X receptors elicits glutamate release from sensory neuron synapses. Nature. 1997;389(6652):749-53.
Gu, J. G., & MacDermott, A. B. (1997). Activation of ATP P2X receptors elicits glutamate release from sensory neuron synapses. Nature, 389(6652), 749-53.
Gu JG, MacDermott AB. Activation of ATP P2X Receptors Elicits Glutamate Release From Sensory Neuron Synapses. Nature. 1997 Oct 16;389(6652):749-53. PubMed PMID: 9338789.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activation of ATP P2X receptors elicits glutamate release from sensory neuron synapses. AU - Gu,J G, AU - MacDermott,A B, PY - 1997/10/24/pubmed PY - 2001/3/23/medline PY - 1997/10/24/entrez SP - 749 EP - 53 JF - Nature JO - Nature VL - 389 IS - 6652 N2 - Painful stimuli to the skin initiate action potentials in the peripheral terminals of dorsal root ganglion (DRG) neurons. These action potentials propagate to DRG central terminals in the dorsal horn of the spinal cord, evoking release of excitatory transmitters such as glutamate onto postsynaptic dorsal horn neurons. P2X receptors, a family of ligand-gated ion channels activated by the endogenous ligand ATP, are highly expressed by DRG neurons. Immunoreactivity to P2X receptors has been identified in the dorsal horn superficial laminae associated with nociceptive DRG central terminals, suggesting the presence of presynaptic P2X receptors. Here we have used a DRG-dorsal horn co-culture system to show that P2X receptors are localized at presynaptic sites on DRG neurons; that activation of these receptors results in increased frequency of spontaneous glutamate release; and that activation of P2X receptors at or near presynaptic DRG nerve terminals elicits action potentials that cause evoked glutamate release. Thus activation of P2X receptors at DRG central terminals can modify sensory signal throughput, and might even initiate sensory signals at central synapses without direct peripheral input. This putative central modulation and generation of sensory signals may be associated with physiological and pathological pain sensation, making presynaptic P2X receptors a possible target for pain therapy. SN - 0028-0836 UR - https://www.unboundmedicine.com/medline/citation/9338789/Activation_of_ATP_P2X_receptors_elicits_glutamate_release_from_sensory_neuron_synapses_ L2 - https://doi.org/10.1038/39639 DB - PRIME DP - Unbound Medicine ER -