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The p38 mitogen-activated protein kinase pathway in activated and anergic Th1 cells.
Cell Immunol. 1997 Sep 15; 180(2):116-23.CI

Abstract

Stimulation of T cells through the TCR leads to activation of the mitogen-activated protein kinase (MAPK) family members ERK (extracellular signal-regulated kinase) and JNK (jun NH2-terminal kinase). These kinases act in synergy to increase the activity of the transcription factor AP-1 which is involved in the transcriptional upregulation of IL-2. Recently a third MAPK member, p38, has been identified. The effects of T cell activation on this pathway have not yet been elucidated. Using two murine Th1 clones, we demonstrate that the p38 pathway is induced upon anti-CD3 plus anti-CD28 crosslinking or PMA plus ionomycin stimulation. p38 activity was induced fully by anti-CD3 or PMA alone and is not enhanced by costimulation even at low levels of TCR signaling. p38 activity peaked at 20 min and was significantly decreased by 2 hr. Anergic (tolerant) Th1 cells showed decreased p38 activity as well as decreased ERK and JNK activities even though levels of these proteins remained unchanged.

Authors+Show Affiliations

Inflammatory Diseases Research, Dupont Merck Pharmaceutical Company, Wilmington, Delaware 19880-0400, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9341741

Citation

DeSilva, D R., et al. "The P38 Mitogen-activated Protein Kinase Pathway in Activated and Anergic Th1 Cells." Cellular Immunology, vol. 180, no. 2, 1997, pp. 116-23.
DeSilva DR, Jones EA, Feeser WS, et al. The p38 mitogen-activated protein kinase pathway in activated and anergic Th1 cells. Cell Immunol. 1997;180(2):116-23.
DeSilva, D. R., Jones, E. A., Feeser, W. S., Manos, E. J., & Scherle, P. A. (1997). The p38 mitogen-activated protein kinase pathway in activated and anergic Th1 cells. Cellular Immunology, 180(2), 116-23.
DeSilva DR, et al. The P38 Mitogen-activated Protein Kinase Pathway in Activated and Anergic Th1 Cells. Cell Immunol. 1997 Sep 15;180(2):116-23. PubMed PMID: 9341741.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The p38 mitogen-activated protein kinase pathway in activated and anergic Th1 cells. AU - DeSilva,D R, AU - Jones,E A, AU - Feeser,W S, AU - Manos,E J, AU - Scherle,P A, PY - 1997/10/28/pubmed PY - 1997/10/28/medline PY - 1997/10/28/entrez SP - 116 EP - 23 JF - Cellular immunology JO - Cell Immunol VL - 180 IS - 2 N2 - Stimulation of T cells through the TCR leads to activation of the mitogen-activated protein kinase (MAPK) family members ERK (extracellular signal-regulated kinase) and JNK (jun NH2-terminal kinase). These kinases act in synergy to increase the activity of the transcription factor AP-1 which is involved in the transcriptional upregulation of IL-2. Recently a third MAPK member, p38, has been identified. The effects of T cell activation on this pathway have not yet been elucidated. Using two murine Th1 clones, we demonstrate that the p38 pathway is induced upon anti-CD3 plus anti-CD28 crosslinking or PMA plus ionomycin stimulation. p38 activity was induced fully by anti-CD3 or PMA alone and is not enhanced by costimulation even at low levels of TCR signaling. p38 activity peaked at 20 min and was significantly decreased by 2 hr. Anergic (tolerant) Th1 cells showed decreased p38 activity as well as decreased ERK and JNK activities even though levels of these proteins remained unchanged. SN - 0008-8749 UR - https://www.unboundmedicine.com/medline/citation/9341741/The_p38_mitogen_activated_protein_kinase_pathway_in_activated_and_anergic_Th1_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0008-8749(97)91182-5 DB - PRIME DP - Unbound Medicine ER -