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Phytoestrogen concentration determines effects on DNA synthesis in human breast cancer cells.
Nutr Cancer 1997; 28(3):236-47NC

Abstract

Thirteen isoflavonoids, flavonoids, and lignans, including some known phytoestrogens, were evaluated for their effects on DNA synthesis in estrogen-dependent (MCF-7) and -independent (MDA-MB-231) human breast cancer cells. Treatment for 24 hours with most of the compounds at 20-80 microM sharply inhibited DNA synthesis in MDA-MB-231 cells. In MCF-7 cells, on the other hand, biphasic effects were seen. At 0.1-10 microM, coumestrol, genistein, biochanin A, apigenin, luteolin, kaempferol, and enterolactone induced DNA synthesis 150-235% and, at 20-90 microM, inhibited DNA synthesis by 50%. Treatment of MCF-7 cells for 10 days with genistein or coumestrol showed continuous stimulation of DNA synthesis at low concentrations. Time-course experiments with genistein in MCF-7 cells showed effects to be reversed by 48-hour withdrawal of genistein at most concentrations. Induction of DNA synthesis in MCF-7 cells, but not in MDA-MB-231 cells, is consistent with an estrogenic effect of these compounds. Inhibition of estrogen-dependent and -independent breast cancer cells at high concentrations suggests additional mechanisms independent of the estrogen receptor. The current focus on the role of phytoestrogens in cancer prevention must take into account the biphasic effects observed in this study, showing inhibition of DNA synthesis at high concentrations but induction at concentrations close to probable levels in humans.

Authors+Show Affiliations

Department of Food Science and Nutrition, University of Minnesota, St. Paul 55108, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9343831

Citation

Wang, C, and M S. Kurzer. "Phytoestrogen Concentration Determines Effects On DNA Synthesis in Human Breast Cancer Cells." Nutrition and Cancer, vol. 28, no. 3, 1997, pp. 236-47.
Wang C, Kurzer MS. Phytoestrogen concentration determines effects on DNA synthesis in human breast cancer cells. Nutr Cancer. 1997;28(3):236-47.
Wang, C., & Kurzer, M. S. (1997). Phytoestrogen concentration determines effects on DNA synthesis in human breast cancer cells. Nutrition and Cancer, 28(3), pp. 236-47.
Wang C, Kurzer MS. Phytoestrogen Concentration Determines Effects On DNA Synthesis in Human Breast Cancer Cells. Nutr Cancer. 1997;28(3):236-47. PubMed PMID: 9343831.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phytoestrogen concentration determines effects on DNA synthesis in human breast cancer cells. AU - Wang,C, AU - Kurzer,M S, PY - 1997/1/1/pubmed PY - 1997/10/31/medline PY - 1997/1/1/entrez SP - 236 EP - 47 JF - Nutrition and cancer JO - Nutr Cancer VL - 28 IS - 3 N2 - Thirteen isoflavonoids, flavonoids, and lignans, including some known phytoestrogens, were evaluated for their effects on DNA synthesis in estrogen-dependent (MCF-7) and -independent (MDA-MB-231) human breast cancer cells. Treatment for 24 hours with most of the compounds at 20-80 microM sharply inhibited DNA synthesis in MDA-MB-231 cells. In MCF-7 cells, on the other hand, biphasic effects were seen. At 0.1-10 microM, coumestrol, genistein, biochanin A, apigenin, luteolin, kaempferol, and enterolactone induced DNA synthesis 150-235% and, at 20-90 microM, inhibited DNA synthesis by 50%. Treatment of MCF-7 cells for 10 days with genistein or coumestrol showed continuous stimulation of DNA synthesis at low concentrations. Time-course experiments with genistein in MCF-7 cells showed effects to be reversed by 48-hour withdrawal of genistein at most concentrations. Induction of DNA synthesis in MCF-7 cells, but not in MDA-MB-231 cells, is consistent with an estrogenic effect of these compounds. Inhibition of estrogen-dependent and -independent breast cancer cells at high concentrations suggests additional mechanisms independent of the estrogen receptor. The current focus on the role of phytoestrogens in cancer prevention must take into account the biphasic effects observed in this study, showing inhibition of DNA synthesis at high concentrations but induction at concentrations close to probable levels in humans. SN - 0163-5581 UR - https://www.unboundmedicine.com/medline/citation/9343831/Phytoestrogen_concentration_determines_effects_on_DNA_synthesis_in_human_breast_cancer_cells_ L2 - http://www.tandfonline.com/doi/full/10.1080/01635589709514582 DB - PRIME DP - Unbound Medicine ER -