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Immunoglobulin M antibody response to measles virus following natural virus infection, primary vaccination, and reexposure to the virus.
Viral Immunol. 1997; 10(3):165-73.VI

Abstract

Evaluation of the measles virus ELISA kit (Merck) to detect specific IgM as an indicator of primary measles antibody response was carried out. A modification of the manufacturer's cutoff value interpretation was introduced to allow for equivocal results in addition to positive and negative ones. With this modification, the test assayed gave an overall reproducibility of 96.16%. The IgM seropositivity rate for seroneutralization-confirmed measles cases was 100% for naturally infected measles subjects and 90% for primary measles vaccinated subjects. Individuals with positive neutralizing antimeasles antibodies in close contact with a confirmed measles case gave the following measles IgM ELISA results: 54.54% negative, 9.09% positive, and 36.36% equivocal, showing a booster with IgM antibody response on reexposure to the virus. Positive subjects with neutralizing antimeasles antibodies without recent contact with a measles case gave negative IgM results. IgM seropositivity was strongly associated with IgG seroconversion and clinical measles (p < 0.0001). The technique assayed performed adequately for the confirmation of both measles natural infection and primary vaccination and for the differentiation of primary and secondary antibody response, taking into account the modification in the cutoff value interpretation introduced and providing that the serum samples are obtained between days 5 and 30 after onset of rash.

Authors+Show Affiliations

Instituto de Virología Dr. J.M. Vanella, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Ciudad Universitaria, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9344339

Citation

Nates, S, et al. "Immunoglobulin M Antibody Response to Measles Virus Following Natural Virus Infection, Primary Vaccination, and Reexposure to the Virus." Viral Immunology, vol. 10, no. 3, 1997, pp. 165-73.
Nates S, Rey G, Giordano M, et al. Immunoglobulin M antibody response to measles virus following natural virus infection, primary vaccination, and reexposure to the virus. Viral Immunol. 1997;10(3):165-73.
Nates, S., Rey, G., Giordano, M., Medeot, S., Depetris, A., Boshell, J., & de Wolff, C. D. (1997). Immunoglobulin M antibody response to measles virus following natural virus infection, primary vaccination, and reexposure to the virus. Viral Immunology, 10(3), 165-73.
Nates S, et al. Immunoglobulin M Antibody Response to Measles Virus Following Natural Virus Infection, Primary Vaccination, and Reexposure to the Virus. Viral Immunol. 1997;10(3):165-73. PubMed PMID: 9344339.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunoglobulin M antibody response to measles virus following natural virus infection, primary vaccination, and reexposure to the virus. AU - Nates,S, AU - Rey,G, AU - Giordano,M, AU - Medeot,S, AU - Depetris,A, AU - Boshell,J, AU - de Wolff,C D, PY - 1997/1/1/pubmed PY - 1997/10/31/medline PY - 1997/1/1/entrez SP - 165 EP - 73 JF - Viral immunology JO - Viral Immunol VL - 10 IS - 3 N2 - Evaluation of the measles virus ELISA kit (Merck) to detect specific IgM as an indicator of primary measles antibody response was carried out. A modification of the manufacturer's cutoff value interpretation was introduced to allow for equivocal results in addition to positive and negative ones. With this modification, the test assayed gave an overall reproducibility of 96.16%. The IgM seropositivity rate for seroneutralization-confirmed measles cases was 100% for naturally infected measles subjects and 90% for primary measles vaccinated subjects. Individuals with positive neutralizing antimeasles antibodies in close contact with a confirmed measles case gave the following measles IgM ELISA results: 54.54% negative, 9.09% positive, and 36.36% equivocal, showing a booster with IgM antibody response on reexposure to the virus. Positive subjects with neutralizing antimeasles antibodies without recent contact with a measles case gave negative IgM results. IgM seropositivity was strongly associated with IgG seroconversion and clinical measles (p < 0.0001). The technique assayed performed adequately for the confirmation of both measles natural infection and primary vaccination and for the differentiation of primary and secondary antibody response, taking into account the modification in the cutoff value interpretation introduced and providing that the serum samples are obtained between days 5 and 30 after onset of rash. SN - 0882-8245 UR - https://www.unboundmedicine.com/medline/citation/9344339/Immunoglobulin_M_antibody_response_to_measles_virus_following_natural_virus_infection_primary_vaccination_and_reexposure_to_the_virus_ L2 - https://www.liebertpub.com/doi/10.1089/vim.1997.10.165?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -