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Spontaneous and inducible production of leukaemia inhibitory factor by human bone marrow stromal cells.
Cytokine. 1997 Oct; 9(10):754-8.C

Abstract

Leukaemia inhibitory factor (LIF) acts on the growth and differentiation of haematopoietic cells. By using a specific enzyme-linked immunosorbent assay for human LIF, we demonstrate that human bone marrow stromal cells produce LIF. LIF synthesis is enhanced in a dose-dependent manner after stimulation with lipopolysaccharide (LPS) and phorbol 12-myristate 13-acetate (PMAS). LIF production in response to PMA is PKC-dependent since the two PKC inhibitors sphingosine and staurosporine markedly diminished it. Interleukin 1alpha (IL-1alpha), IL-1beta, IL-3, IL-6, IL-8, tumour necrosis factor (TNF-alpha) and SCF (both at 10 ng/ml) stimulate LIF production. By contrast macrophage colony-stimulating factor (M-CSF), granulocyte (G)-CSF, GM-CSF, basic fibroblast growth factor (bFGF), platelet-activating factor (PAF), protaglandin E2 (PGE2), leukotriene B4 (LTB4), and leukotriene C4 (LTC4) did not. These results suggest that bone marrow stromal cells might represent a major source for the cytokine-regulated local production of LIF inside human bone marrow.

Authors+Show Affiliations

Laboratoire d'Hématologie Expérimentale,, Faculté de Médecine, 2 rue Dr. Marcland, Limoges, 87025, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9344507

Citation

Lorgeot, V, et al. "Spontaneous and Inducible Production of Leukaemia Inhibitory Factor By Human Bone Marrow Stromal Cells." Cytokine, vol. 9, no. 10, 1997, pp. 754-8.
Lorgeot V, Rougier F, Fixe P, et al. Spontaneous and inducible production of leukaemia inhibitory factor by human bone marrow stromal cells. Cytokine. 1997;9(10):754-8.
Lorgeot, V., Rougier, F., Fixe, P., Cornu, E., Praloran, V., & Denizot, Y. (1997). Spontaneous and inducible production of leukaemia inhibitory factor by human bone marrow stromal cells. Cytokine, 9(10), 754-8.
Lorgeot V, et al. Spontaneous and Inducible Production of Leukaemia Inhibitory Factor By Human Bone Marrow Stromal Cells. Cytokine. 1997;9(10):754-8. PubMed PMID: 9344507.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spontaneous and inducible production of leukaemia inhibitory factor by human bone marrow stromal cells. AU - Lorgeot,V, AU - Rougier,F, AU - Fixe,P, AU - Cornu,E, AU - Praloran,V, AU - Denizot,Y, PY - 1997/11/5/pubmed PY - 1997/11/5/medline PY - 1997/11/5/entrez SP - 754 EP - 8 JF - Cytokine JO - Cytokine VL - 9 IS - 10 N2 - Leukaemia inhibitory factor (LIF) acts on the growth and differentiation of haematopoietic cells. By using a specific enzyme-linked immunosorbent assay for human LIF, we demonstrate that human bone marrow stromal cells produce LIF. LIF synthesis is enhanced in a dose-dependent manner after stimulation with lipopolysaccharide (LPS) and phorbol 12-myristate 13-acetate (PMAS). LIF production in response to PMA is PKC-dependent since the two PKC inhibitors sphingosine and staurosporine markedly diminished it. Interleukin 1alpha (IL-1alpha), IL-1beta, IL-3, IL-6, IL-8, tumour necrosis factor (TNF-alpha) and SCF (both at 10 ng/ml) stimulate LIF production. By contrast macrophage colony-stimulating factor (M-CSF), granulocyte (G)-CSF, GM-CSF, basic fibroblast growth factor (bFGF), platelet-activating factor (PAF), protaglandin E2 (PGE2), leukotriene B4 (LTB4), and leukotriene C4 (LTC4) did not. These results suggest that bone marrow stromal cells might represent a major source for the cytokine-regulated local production of LIF inside human bone marrow. SN - 1043-4666 UR - https://www.unboundmedicine.com/medline/citation/9344507/Spontaneous_and_inducible_production_of_leukaemia_inhibitory_factor_by_human_bone_marrow_stromal_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1043-4666(97)90225-8 DB - PRIME DP - Unbound Medicine ER -