Tags

Type your tag names separated by a space and hit enter

Enhancement of ColE1-type replication promoted by the Gp alpha initiator protein of bacteriophage P4.
FEMS Microbiol Lett. 1997 Oct 01; 155(1):11-5.FM

Abstract

Gp alpha, the phage P4 specific replication protein, increases in vitro replication of pCN51, a pBR322 based replicon, by a factor of two. This effect is dependent on DNA polymerase I and requires transcription by host RNA polymerase. Electron microscopic analysis of replicating intermediates indicates that pCN51 replication occurred from the same origin and with the same directionality in the presence and in the absence of Gp alpha. These results reveal that Gp alpha can influence the replication of an heterologous replicon and show that this effect occurs in ColE1-type replicons without altering the normal pattern of initiation. Further analysis of replicating intermediates shows an increase in the average size of the ColE1-type replication 'bubble' obtained in the presence of Gp alpha. It is proposed that Gp alpha interacts with the ColE1 replisome complex at an early replication stage.

Authors+Show Affiliations

Centro de Investigaciones Biológicas, C.S.I.C., Madrid, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9345759

Citation

Díaz-Orejas, R, et al. "Enhancement of ColE1-type Replication Promoted By the Gp Alpha Initiator Protein of Bacteriophage P4." FEMS Microbiology Letters, vol. 155, no. 1, 1997, pp. 11-5.
Díaz-Orejas R, Lurz R, Rückert B. Enhancement of ColE1-type replication promoted by the Gp alpha initiator protein of bacteriophage P4. FEMS Microbiol Lett. 1997;155(1):11-5.
Díaz-Orejas, R., Lurz, R., & Rückert, B. (1997). Enhancement of ColE1-type replication promoted by the Gp alpha initiator protein of bacteriophage P4. FEMS Microbiology Letters, 155(1), 11-5.
Díaz-Orejas R, Lurz R, Rückert B. Enhancement of ColE1-type Replication Promoted By the Gp Alpha Initiator Protein of Bacteriophage P4. FEMS Microbiol Lett. 1997 Oct 1;155(1):11-5. PubMed PMID: 9345759.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhancement of ColE1-type replication promoted by the Gp alpha initiator protein of bacteriophage P4. AU - Díaz-Orejas,R, AU - Lurz,R, AU - Rückert,B, PY - 1997/11/5/pubmed PY - 1997/11/5/medline PY - 1997/11/5/entrez SP - 11 EP - 5 JF - FEMS microbiology letters JO - FEMS Microbiol. Lett. VL - 155 IS - 1 N2 - Gp alpha, the phage P4 specific replication protein, increases in vitro replication of pCN51, a pBR322 based replicon, by a factor of two. This effect is dependent on DNA polymerase I and requires transcription by host RNA polymerase. Electron microscopic analysis of replicating intermediates indicates that pCN51 replication occurred from the same origin and with the same directionality in the presence and in the absence of Gp alpha. These results reveal that Gp alpha can influence the replication of an heterologous replicon and show that this effect occurs in ColE1-type replicons without altering the normal pattern of initiation. Further analysis of replicating intermediates shows an increase in the average size of the ColE1-type replication 'bubble' obtained in the presence of Gp alpha. It is proposed that Gp alpha interacts with the ColE1 replisome complex at an early replication stage. SN - 0378-1097 UR - https://www.unboundmedicine.com/medline/citation/9345759/Enhancement_of_ColE1_type_replication_promoted_by_the_Gp_alpha_initiator_protein_of_bacteriophage_P4_ DB - PRIME DP - Unbound Medicine ER -