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Adherence of oral streptococci to an immobilized antimicrobial agent.
Arch Oral Biol 1997; 42(8):539-45AO

Abstract

An antimicrobial agent, 3-(trimethoxysilyl)-propyldimethyloctadecyl ammonium chloride, was immobilized on silica. Interaction between the material (termed) OAIS) and various oral bacterial species were then studied. Seven species of Streptococcus and two Actinomyces were investigated for their ability to adhere to this biomaterial. Cell-surface hydrophobicity and zeta-potential were examined as well. Analysis of extracted hydrophobic proteins which adhered to OAIS revealed that the adherence of these micro-organisms was closely related to the hydrophobicity of their cell surfaces. The results of zeta-potential assays indicated that negative charge on the cell surface inhibited adherence to OAIS. Gel electrophoresis revealed that OAIS could absorb cell-surface hydrophobic proteins from all bacterial species tested. Preadsorption of hydrophobic components on the cell surface inhibited adherence of the Strep. mutans strain to OAIS in a dose-dependent manner. The results indicate that OAIS adsorption of these oral bacteria was dependent on the degree of hydrophobicity of their surfaces. A major component of this adherence was hydrophobic cell-surface proteins.

Authors+Show Affiliations

Research and Development Department, Sunstar Inc., Osaka, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9347116

Citation

Saito, T, et al. "Adherence of Oral Streptococci to an Immobilized Antimicrobial Agent." Archives of Oral Biology, vol. 42, no. 8, 1997, pp. 539-45.
Saito T, Takatsuka T, Kato T, et al. Adherence of oral streptococci to an immobilized antimicrobial agent. Arch Oral Biol. 1997;42(8):539-45.
Saito, T., Takatsuka, T., Kato, T., Ishihara, K., & Okuda, K. (1997). Adherence of oral streptococci to an immobilized antimicrobial agent. Archives of Oral Biology, 42(8), pp. 539-45.
Saito T, et al. Adherence of Oral Streptococci to an Immobilized Antimicrobial Agent. Arch Oral Biol. 1997;42(8):539-45. PubMed PMID: 9347116.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adherence of oral streptococci to an immobilized antimicrobial agent. AU - Saito,T, AU - Takatsuka,T, AU - Kato,T, AU - Ishihara,K, AU - Okuda,K, PY - 1997/8/1/pubmed PY - 1997/11/5/medline PY - 1997/8/1/entrez SP - 539 EP - 45 JF - Archives of oral biology JO - Arch. Oral Biol. VL - 42 IS - 8 N2 - An antimicrobial agent, 3-(trimethoxysilyl)-propyldimethyloctadecyl ammonium chloride, was immobilized on silica. Interaction between the material (termed) OAIS) and various oral bacterial species were then studied. Seven species of Streptococcus and two Actinomyces were investigated for their ability to adhere to this biomaterial. Cell-surface hydrophobicity and zeta-potential were examined as well. Analysis of extracted hydrophobic proteins which adhered to OAIS revealed that the adherence of these micro-organisms was closely related to the hydrophobicity of their cell surfaces. The results of zeta-potential assays indicated that negative charge on the cell surface inhibited adherence to OAIS. Gel electrophoresis revealed that OAIS could absorb cell-surface hydrophobic proteins from all bacterial species tested. Preadsorption of hydrophobic components on the cell surface inhibited adherence of the Strep. mutans strain to OAIS in a dose-dependent manner. The results indicate that OAIS adsorption of these oral bacteria was dependent on the degree of hydrophobicity of their surfaces. A major component of this adherence was hydrophobic cell-surface proteins. SN - 0003-9969 UR - https://www.unboundmedicine.com/medline/citation/9347116/Adherence_of_oral_streptococci_to_an_immobilized_antimicrobial_agent_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-9969(97)00054-X DB - PRIME DP - Unbound Medicine ER -