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Complete amino acid sequence of ananain and a comparison with stem bromelain and other plant cysteine proteases.
Biochem J. 1997 Oct 01; 327 (Pt 1):199-202.BJ

Abstract

The amino acid sequences of ananain (EC3.4.22.31) and stem bromelain (3.4.22.32), two cysteine proteases from pineapple stem, are similar yet ananain and stem bromelain possess distinct specificities towards synthetic peptide substrates and different reactivities towards the cysteine protease inhibitors E-64 and chicken egg white cystatin. We present here the complete amino acid sequence of ananain and compare it with the reported sequences of pineapple stem bromelain, papain and chymopapain from papaya and actinidin from kiwifruit. Ananain is comprised of 216 residues with a theoretical mass of 23464 Da. This primary structure includes a sequence insert between residues 170 and 174 not present in stem bromelain or papain and a hydrophobic series of amino acids adjacent to His-157. It is possible that these sequence differences contribute to the different substrate and inhibitor specificities exhibited by ananain and stem bromelain.

Authors+Show Affiliations

Genzyme Corporation, 1 Mountain Road, Framingham, MA 01701-9322, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

9355753

Citation

Lee, K L., et al. "Complete Amino Acid Sequence of Ananain and a Comparison With Stem Bromelain and Other Plant Cysteine Proteases." The Biochemical Journal, vol. 327 (Pt 1), 1997, pp. 199-202.
Lee KL, Albee KL, Bernasconi RJ, et al. Complete amino acid sequence of ananain and a comparison with stem bromelain and other plant cysteine proteases. Biochem J. 1997;327 (Pt 1):199-202.
Lee, K. L., Albee, K. L., Bernasconi, R. J., & Edmunds, T. (1997). Complete amino acid sequence of ananain and a comparison with stem bromelain and other plant cysteine proteases. The Biochemical Journal, 327 (Pt 1), 199-202.
Lee KL, et al. Complete Amino Acid Sequence of Ananain and a Comparison With Stem Bromelain and Other Plant Cysteine Proteases. Biochem J. 1997 Oct 1;327 (Pt 1):199-202. PubMed PMID: 9355753.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Complete amino acid sequence of ananain and a comparison with stem bromelain and other plant cysteine proteases. AU - Lee,K L, AU - Albee,K L, AU - Bernasconi,R J, AU - Edmunds,T, PY - 1997/11/14/pubmed PY - 1997/11/14/medline PY - 1997/11/14/entrez SP - 199 EP - 202 JF - The Biochemical journal JO - Biochem. J. VL - 327 (Pt 1) N2 - The amino acid sequences of ananain (EC3.4.22.31) and stem bromelain (3.4.22.32), two cysteine proteases from pineapple stem, are similar yet ananain and stem bromelain possess distinct specificities towards synthetic peptide substrates and different reactivities towards the cysteine protease inhibitors E-64 and chicken egg white cystatin. We present here the complete amino acid sequence of ananain and compare it with the reported sequences of pineapple stem bromelain, papain and chymopapain from papaya and actinidin from kiwifruit. Ananain is comprised of 216 residues with a theoretical mass of 23464 Da. This primary structure includes a sequence insert between residues 170 and 174 not present in stem bromelain or papain and a hydrophobic series of amino acids adjacent to His-157. It is possible that these sequence differences contribute to the different substrate and inhibitor specificities exhibited by ananain and stem bromelain. SN - 0264-6021 UR - https://www.unboundmedicine.com/medline/citation/9355753/Complete_amino_acid_sequence_of_ananain_and_a_comparison_with_stem_bromelain_and_other_plant_cysteine_proteases_ L2 - https://portlandpress.com/biochemj/article-lookup/doi/10.1042/bj3270199 DB - PRIME DP - Unbound Medicine ER -