Tags

Type your tag names separated by a space and hit enter

Mitogenic effects of epidermal growth factor and platelet-derived growth factor on canine and equine mesangial cells in vitro.
Am J Vet Res. 1997 Nov; 58(11):1308-13.AJ

Abstract

OBJECTIVE

To evaluate the effects of epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) on canine and equine mesangial cell (MC) proliferation in vitro.

SAMPLE POPULATION

Third- through eighth-passage canine and equine MC were obtained from explant outgrowth after differential sieving of glomeruli isolated from the kidneys of clinically normal dogs and horses.

PROCEDURE

Mitogenic effects of serum, insulin, EGF, and PDGF were evaluated in MC by induction of DNA synthesis, measured as stimulation of [3H]thymidine incorporation and increase in cell numbers.

RESULTS

Epidermal growth factor was a potent mitogen in canine and equine MC, stimulating dose-dependent increases in [3H]thymidine incorporation and moderate increase in cell numbers. Although PDGF alone did not significantly stimulate [3H]thymidine incorporation in canine MC, PDGF was synergistic with EGF in stimulation of [3H]thymidine incorporation in canine and equine MC, and PDGF significantly stimulated [3H]thymidine incorporation in equine MC.

CONCLUSIONS

Both EGF and PDGF are important mediators of canine and equine MC proliferation.

CLINICAL RELEVANCE

Our results are consistent with the hypothesis that growth factors have a role in the progression of glomerular disease in dogs and horses. These findings could prove to be of diagnostic, prognostic, and therapeutic value in the management of spontaneous renal disease in these species.

Authors+Show Affiliations

Ennulat D
Department of Physiology, College of Veterinary Medicine, University of Georgia, Athens 30602, USA.
Brown CA
No affiliation info available
Brown SA
No affiliation info available

MeSH

AnimalsBlood ProteinsCell DivisionCells, CulturedDNADogsDose-Response Relationship, DrugEpidermal Growth FactorFemaleGlomerular MesangiumHorsesInsulinMalePlatelet-Derived Growth FactorThymidineTritium

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9361897

Citation

Ennulat, D, et al. "Mitogenic Effects of Epidermal Growth Factor and Platelet-derived Growth Factor On Canine and Equine Mesangial Cells in Vitro." American Journal of Veterinary Research, vol. 58, no. 11, 1997, pp. 1308-13.
Ennulat D, Brown CA, Brown SA. Mitogenic effects of epidermal growth factor and platelet-derived growth factor on canine and equine mesangial cells in vitro. Am J Vet Res. 1997;58(11):1308-13.
Ennulat, D., Brown, C. A., & Brown, S. A. (1997). Mitogenic effects of epidermal growth factor and platelet-derived growth factor on canine and equine mesangial cells in vitro. American Journal of Veterinary Research, 58(11), 1308-13.
Ennulat D, Brown CA, Brown SA. Mitogenic Effects of Epidermal Growth Factor and Platelet-derived Growth Factor On Canine and Equine Mesangial Cells in Vitro. Am J Vet Res. 1997;58(11):1308-13. PubMed PMID: 9361897.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mitogenic effects of epidermal growth factor and platelet-derived growth factor on canine and equine mesangial cells in vitro. AU - Ennulat,D, AU - Brown,C A, AU - Brown,S A, PY - 1997/11/15/pubmed PY - 1997/11/15/medline PY - 1997/11/15/entrez SP - 1308 EP - 13 JF - American journal of veterinary research JO - Am J Vet Res VL - 58 IS - 11 N2 - OBJECTIVE: To evaluate the effects of epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) on canine and equine mesangial cell (MC) proliferation in vitro. SAMPLE POPULATION: Third- through eighth-passage canine and equine MC were obtained from explant outgrowth after differential sieving of glomeruli isolated from the kidneys of clinically normal dogs and horses. PROCEDURE: Mitogenic effects of serum, insulin, EGF, and PDGF were evaluated in MC by induction of DNA synthesis, measured as stimulation of [3H]thymidine incorporation and increase in cell numbers. RESULTS: Epidermal growth factor was a potent mitogen in canine and equine MC, stimulating dose-dependent increases in [3H]thymidine incorporation and moderate increase in cell numbers. Although PDGF alone did not significantly stimulate [3H]thymidine incorporation in canine MC, PDGF was synergistic with EGF in stimulation of [3H]thymidine incorporation in canine and equine MC, and PDGF significantly stimulated [3H]thymidine incorporation in equine MC. CONCLUSIONS: Both EGF and PDGF are important mediators of canine and equine MC proliferation. CLINICAL RELEVANCE: Our results are consistent with the hypothesis that growth factors have a role in the progression of glomerular disease in dogs and horses. These findings could prove to be of diagnostic, prognostic, and therapeutic value in the management of spontaneous renal disease in these species. SN - 0002-9645 UR - https://www.unboundmedicine.com/medline/citation/9361897/Mitogenic_effects_of_epidermal_growth_factor_and_platelet_derived_growth_factor_on_canine_and_equine_mesangial_cells_in_vitro_ DB - PRIME DP - Unbound Medicine ER -