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Initiation of periovulatory events in gonadotrophin-stimulated macaques with varying doses of recombinant human chorionic gonadotrophin.
Hum Reprod. 1997 Sep; 12(9):1877-85.HR

Abstract

During in-vitro fertilization (IVF) cycles, a large bolus of human chorionic gonadotrophin (HCG) is used to induce periovulatory events, but the efficacy of lower doses is undefined. Following follicular stimulation in rhesus monkeys, oocyte nuclear maturation, IVF, granulosa cell luteinization and corpus luteum function were compared after injection of 100, 300 or 1000 IU recombinant HCG or 1000 IU urinary HCG. Bioactive HCG rose to peak concentrations within 2 h that were proportional to the dose administered (100 < 300 < 1000 IU, recombinant HCG = urinary HCG). The duration of surge values (>100 ng/ml) was also dose-dependent (0 h, 100 IU; 24 h, 300 IU; >48 h, 1000 IU, recombinant and urinary HCG). While the proportions of oocytes resuming meiosis and undergoing IVF were similar among groups, fewer animals yielded fertilizable oocytes following 100 and 300 IU (five of nine) compared to 1000 IU recombinant and urinary HCG (nine of 10). Peak values of serum progesterone in the luteal phase were similar, but declined 2 days earlier after 100 and 300 IU relative to 1000 IU recombinant and urinary HCG. Thus, 3-10 fold lower doses of HCG elicit low amplitude surges of short duration that induce periovulatory events such as re-initiation of oocyte meiosis and granulosa cell luteinization. However, oocyte fertilization and luteal function may optimally require surges of higher amplitude and longer duration similar to those produced by standard doses of 1000 IU recombinant or urinary HCG.

Authors+Show Affiliations

Oregon Regional Primate Research Center, Beaverton 97006, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9363699

Citation

Zelinski-Wooten, M B., et al. "Initiation of Periovulatory Events in Gonadotrophin-stimulated Macaques With Varying Doses of Recombinant Human Chorionic Gonadotrophin." Human Reproduction (Oxford, England), vol. 12, no. 9, 1997, pp. 1877-85.
Zelinski-Wooten MB, Hutchison JS, Trinchard-Lugan I, et al. Initiation of periovulatory events in gonadotrophin-stimulated macaques with varying doses of recombinant human chorionic gonadotrophin. Hum Reprod. 1997;12(9):1877-85.
Zelinski-Wooten, M. B., Hutchison, J. S., Trinchard-Lugan, I., Hess, D. L., Wolf, D. P., & Stouffer, R. L. (1997). Initiation of periovulatory events in gonadotrophin-stimulated macaques with varying doses of recombinant human chorionic gonadotrophin. Human Reproduction (Oxford, England), 12(9), 1877-85.
Zelinski-Wooten MB, et al. Initiation of Periovulatory Events in Gonadotrophin-stimulated Macaques With Varying Doses of Recombinant Human Chorionic Gonadotrophin. Hum Reprod. 1997;12(9):1877-85. PubMed PMID: 9363699.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Initiation of periovulatory events in gonadotrophin-stimulated macaques with varying doses of recombinant human chorionic gonadotrophin. AU - Zelinski-Wooten,M B, AU - Hutchison,J S, AU - Trinchard-Lugan,I, AU - Hess,D L, AU - Wolf,D P, AU - Stouffer,R L, PY - 1997/11/18/pubmed PY - 1997/11/18/medline PY - 1997/11/18/entrez SP - 1877 EP - 85 JF - Human reproduction (Oxford, England) JO - Hum Reprod VL - 12 IS - 9 N2 - During in-vitro fertilization (IVF) cycles, a large bolus of human chorionic gonadotrophin (HCG) is used to induce periovulatory events, but the efficacy of lower doses is undefined. Following follicular stimulation in rhesus monkeys, oocyte nuclear maturation, IVF, granulosa cell luteinization and corpus luteum function were compared after injection of 100, 300 or 1000 IU recombinant HCG or 1000 IU urinary HCG. Bioactive HCG rose to peak concentrations within 2 h that were proportional to the dose administered (100 < 300 < 1000 IU, recombinant HCG = urinary HCG). The duration of surge values (>100 ng/ml) was also dose-dependent (0 h, 100 IU; 24 h, 300 IU; >48 h, 1000 IU, recombinant and urinary HCG). While the proportions of oocytes resuming meiosis and undergoing IVF were similar among groups, fewer animals yielded fertilizable oocytes following 100 and 300 IU (five of nine) compared to 1000 IU recombinant and urinary HCG (nine of 10). Peak values of serum progesterone in the luteal phase were similar, but declined 2 days earlier after 100 and 300 IU relative to 1000 IU recombinant and urinary HCG. Thus, 3-10 fold lower doses of HCG elicit low amplitude surges of short duration that induce periovulatory events such as re-initiation of oocyte meiosis and granulosa cell luteinization. However, oocyte fertilization and luteal function may optimally require surges of higher amplitude and longer duration similar to those produced by standard doses of 1000 IU recombinant or urinary HCG. SN - 0268-1161 UR - https://www.unboundmedicine.com/medline/citation/9363699/Initiation_of_periovulatory_events_in_gonadotrophin_stimulated_macaques_with_varying_doses_of_recombinant_human_chorionic_gonadotrophin_ L2 - https://academic.oup.com/humrep/article-lookup/doi/10.1093/humrep/12.9.1877 DB - PRIME DP - Unbound Medicine ER -