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Detection of osteopontin as matrix protein in calcium-containing urinary stones.
Hinyokika Kiyo. 1997 Sep; 43(9):623-7.HK

Abstract

Osteopontin (OPN) has been identified as a matrix protein of calcium oxalate urinary stones by sequencing c-DNA for urinary stone protein. OPN, a phosphoprotein, is susceptible to thrombin digestion and specifically stainable with Stains-All. We examined the matrix in 4 kinds of urinary stones, calcium oxalate dihydrate, calcium phosphate, magnesium ammonium phosphate and uric acid; for the presence of OPN by staining thrombin-digestion and undigested matrix with Stains-All. Matrix was extracted with a 0.1 M ethylenediamine tetraacetic acid (EDTA) solution. Furthermore, the amino acid sequence was determined for the NH2-terminal 20 amino acid residues. OPN was identified in calcium oxalate dihydrate and calcium phosphate stones, but was absent in magnesium ammonium phosphate and uric acid stones. Our findings suggest that OPN, which binds tightly to hydroxyapatite and is related to bone formation as bone matrix, also participates in the formation of calcium-containing urinary stones.

Authors+Show Affiliations

Department of Urology, Kinki University School of Medicine.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9365840

Citation

Yamate, T, et al. "Detection of Osteopontin as Matrix Protein in Calcium-containing Urinary Stones." Hinyokika Kiyo. Acta Urologica Japonica, vol. 43, no. 9, 1997, pp. 623-7.
Yamate T, Umekawa T, Iguchi M, et al. Detection of osteopontin as matrix protein in calcium-containing urinary stones. Hinyokika Kiyo. 1997;43(9):623-7.
Yamate, T., Umekawa, T., Iguchi, M., Kurita, T., & Kohri, K. (1997). Detection of osteopontin as matrix protein in calcium-containing urinary stones. Hinyokika Kiyo. Acta Urologica Japonica, 43(9), 623-7.
Yamate T, et al. Detection of Osteopontin as Matrix Protein in Calcium-containing Urinary Stones. Hinyokika Kiyo. 1997;43(9):623-7. PubMed PMID: 9365840.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of osteopontin as matrix protein in calcium-containing urinary stones. AU - Yamate,T, AU - Umekawa,T, AU - Iguchi,M, AU - Kurita,T, AU - Kohri,K, PY - 1997/11/20/pubmed PY - 1997/11/20/medline PY - 1997/11/20/entrez SP - 623 EP - 7 JF - Hinyokika kiyo. Acta urologica Japonica JO - Hinyokika Kiyo VL - 43 IS - 9 N2 - Osteopontin (OPN) has been identified as a matrix protein of calcium oxalate urinary stones by sequencing c-DNA for urinary stone protein. OPN, a phosphoprotein, is susceptible to thrombin digestion and specifically stainable with Stains-All. We examined the matrix in 4 kinds of urinary stones, calcium oxalate dihydrate, calcium phosphate, magnesium ammonium phosphate and uric acid; for the presence of OPN by staining thrombin-digestion and undigested matrix with Stains-All. Matrix was extracted with a 0.1 M ethylenediamine tetraacetic acid (EDTA) solution. Furthermore, the amino acid sequence was determined for the NH2-terminal 20 amino acid residues. OPN was identified in calcium oxalate dihydrate and calcium phosphate stones, but was absent in magnesium ammonium phosphate and uric acid stones. Our findings suggest that OPN, which binds tightly to hydroxyapatite and is related to bone formation as bone matrix, also participates in the formation of calcium-containing urinary stones. SN - 0018-1994 UR - https://www.unboundmedicine.com/medline/citation/9365840/Detection_of_osteopontin_as_matrix_protein_in_calcium_containing_urinary_stones_ L2 - https://repository.kulib.kyoto-u.ac.jp/dspace/handle/2433/116036 DB - PRIME DP - Unbound Medicine ER -