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Evidence for persistence of human parvovirus B19 DNA in bone marrow.
J Med Virol. 1997 Nov; 53(3):229-32.JM

Abstract

A nested polymerase chain reaction assay (nPCR) was used to investigate the potential of human parvovirus B19 DNA to persist in blood or bone marrow samples obtained either from blood donors or cadaveric bone donors or from patients presenting with clinical signs of parvovirus B19 infection. The presence of parvovirus B19 specific antibody in blood was tested by enzyme immunoassay (EIA). B19 virus genome was not detected in any blood sample of 115 blood donors, of whom 92 (80%) had anti-B19 IgG antibody only as an indication of past infection. In contrast; B19 virus DNA was detected in the bone marrow of 4 out of 45 bone donors. Each one of the serum samples available for 3 of these 4 individuals contained anti-B19 IgG antibody. Among 84 patients with clinical manifestations of parvovirus B19 infection, 17 (20%) had B19 virus DNA in bone marrow. Eight of the latter patients had anti-B19 IgG antibody in their blood but neither anti-B19 IgG nor B19 virus DNA. These data document the ability of parvovirus B19 DNA to persist in the bone marrow of asymptomatic individuals and patients with parvovirus B19 infection suspected on clinical grounds.

Authors+Show Affiliations

Institute for Clinical Microbiology and Immunology, St. Gallen, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9365887

Citation

Cassinotti, P, et al. "Evidence for Persistence of Human Parvovirus B19 DNA in Bone Marrow." Journal of Medical Virology, vol. 53, no. 3, 1997, pp. 229-32.
Cassinotti P, Burtonboy G, Fopp M, et al. Evidence for persistence of human parvovirus B19 DNA in bone marrow. J Med Virol. 1997;53(3):229-32.
Cassinotti, P., Burtonboy, G., Fopp, M., & Siegl, G. (1997). Evidence for persistence of human parvovirus B19 DNA in bone marrow. Journal of Medical Virology, 53(3), 229-32.
Cassinotti P, et al. Evidence for Persistence of Human Parvovirus B19 DNA in Bone Marrow. J Med Virol. 1997;53(3):229-32. PubMed PMID: 9365887.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence for persistence of human parvovirus B19 DNA in bone marrow. AU - Cassinotti,P, AU - Burtonboy,G, AU - Fopp,M, AU - Siegl,G, PY - 1997/11/20/pubmed PY - 2000/6/20/medline PY - 1997/11/20/entrez SP - 229 EP - 32 JF - Journal of medical virology JO - J Med Virol VL - 53 IS - 3 N2 - A nested polymerase chain reaction assay (nPCR) was used to investigate the potential of human parvovirus B19 DNA to persist in blood or bone marrow samples obtained either from blood donors or cadaveric bone donors or from patients presenting with clinical signs of parvovirus B19 infection. The presence of parvovirus B19 specific antibody in blood was tested by enzyme immunoassay (EIA). B19 virus genome was not detected in any blood sample of 115 blood donors, of whom 92 (80%) had anti-B19 IgG antibody only as an indication of past infection. In contrast; B19 virus DNA was detected in the bone marrow of 4 out of 45 bone donors. Each one of the serum samples available for 3 of these 4 individuals contained anti-B19 IgG antibody. Among 84 patients with clinical manifestations of parvovirus B19 infection, 17 (20%) had B19 virus DNA in bone marrow. Eight of the latter patients had anti-B19 IgG antibody in their blood but neither anti-B19 IgG nor B19 virus DNA. These data document the ability of parvovirus B19 DNA to persist in the bone marrow of asymptomatic individuals and patients with parvovirus B19 infection suspected on clinical grounds. SN - 0146-6615 UR - https://www.unboundmedicine.com/medline/citation/9365887/Evidence_for_persistence_of_human_parvovirus_B19_DNA_in_bone_marrow_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0146-6615&date=1997&volume=53&issue=3&spage=229 DB - PRIME DP - Unbound Medicine ER -