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Interdependence in the processing of ribosomal RNAs in Schizosaccharomyces pombe.
J Mol Biol. 1997 Nov 07; 273(4):782-8.JM

Abstract

Eukaryotic rRNAs are produced by cleavage of a large 35 to 45 S pre-rRNA transcript which initially must be fully transcribed and assembled into an 80 to 90 S nucleolar ribonucleoprotein particle. Despite this need for a completed transcript, several investigations have reported a split processing scheme for independent maturation of the large and small subunit rRNAs. Here, an efficiently expressed rDNA plasmid was used to quantitatively analyze the effects of mutations in the internal transcribed spacer (ITS) region in the yeast, Schizosaccharomyces pombe. The results show that substitution of ITS regions inhibits the processing of distant external transcribed spacers (ETS) and that deletion of the ITS2 spacer not only prevents the maturation of the large subunit, but severely affects maturation of the small subunit rRNA. This indicates that the processing mechanisms are not fully split and, when taken together with other evidence of interdependences in rRNA maturation, the results suggest that the interdependences act as a quality control mechanism to help ensure that only functional rRNA is incorporated into ribosomes.

Authors+Show Affiliations

Department of Molecular Biology and Genetics, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9367771

Citation

Good, L, et al. "Interdependence in the Processing of Ribosomal RNAs in Schizosaccharomyces Pombe." Journal of Molecular Biology, vol. 273, no. 4, 1997, pp. 782-8.
Good L, Intine RV, Nazar RN. Interdependence in the processing of ribosomal RNAs in Schizosaccharomyces pombe. J Mol Biol. 1997;273(4):782-8.
Good, L., Intine, R. V., & Nazar, R. N. (1997). Interdependence in the processing of ribosomal RNAs in Schizosaccharomyces pombe. Journal of Molecular Biology, 273(4), 782-8.
Good L, Intine RV, Nazar RN. Interdependence in the Processing of Ribosomal RNAs in Schizosaccharomyces Pombe. J Mol Biol. 1997 Nov 7;273(4):782-8. PubMed PMID: 9367771.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interdependence in the processing of ribosomal RNAs in Schizosaccharomyces pombe. AU - Good,L, AU - Intine,R V, AU - Nazar,R N, PY - 1998/2/12/pubmed PY - 1998/2/12/medline PY - 1998/2/12/entrez SP - 782 EP - 8 JF - Journal of molecular biology JO - J Mol Biol VL - 273 IS - 4 N2 - Eukaryotic rRNAs are produced by cleavage of a large 35 to 45 S pre-rRNA transcript which initially must be fully transcribed and assembled into an 80 to 90 S nucleolar ribonucleoprotein particle. Despite this need for a completed transcript, several investigations have reported a split processing scheme for independent maturation of the large and small subunit rRNAs. Here, an efficiently expressed rDNA plasmid was used to quantitatively analyze the effects of mutations in the internal transcribed spacer (ITS) region in the yeast, Schizosaccharomyces pombe. The results show that substitution of ITS regions inhibits the processing of distant external transcribed spacers (ETS) and that deletion of the ITS2 spacer not only prevents the maturation of the large subunit, but severely affects maturation of the small subunit rRNA. This indicates that the processing mechanisms are not fully split and, when taken together with other evidence of interdependences in rRNA maturation, the results suggest that the interdependences act as a quality control mechanism to help ensure that only functional rRNA is incorporated into ribosomes. SN - 0022-2836 UR - https://www.unboundmedicine.com/medline/citation/9367771/Interdependence_in_the_processing_of_ribosomal_RNAs_in_Schizosaccharomyces_pombe_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2836(97)91351-6 DB - PRIME DP - Unbound Medicine ER -