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Immunohistochemical characterization of retinal glial cell changes in areas of vascular occlusion secondary to diabetic retinopathy.
Acta Ophthalmol Scand. 1997 Aug; 75(4):388-92.AO

Abstract

PURPOSE

To study changes in retinal glial cell components in areas of vascular occlusion secondary to diabetic retinopathy.

MATERIAL

The retina from ten eyes of six diabetic patients and from five eyes of five normal controls were studied for immunoreactivity to glial fibrillary acid protein and vimentin (glial cells), S-100 protein (perivascular glial cells), carbonic anhydrase isoenzyme II and CD-57 antigen (Müller cells), and CD-68 antigen (microglia).

RESULTS

The study showed increased immunoreactivity to S-100 protein, corresponding to perivascularly located glial cells in the retina from diabetic patients, except for areas of vascular occlusion where this immunoreactivity was absent. Furthermore, the material invading the lumen of former retinal vessels in areas of vascular occlusion showed immunoreactivity to CAH-II and CD-57, suggesting that this material represents ingrowth of retinal Müller cells.

CONCLUSIONS

The findings suggest that at least two types of changes in retinal glial cells are involved in the pathophysiology of diabetic retinopathy, i.e. 1) Reactive changes in the perivascular glial cells in the retina, and 2) Müller cell ingrowth into the former lumen of occluded retinal vessels.

Authors+Show Affiliations

Department of Ophthalmology, Arhus University Hospital, Arhus C, Denmark.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9374245

Citation

Bek, T. "Immunohistochemical Characterization of Retinal Glial Cell Changes in Areas of Vascular Occlusion Secondary to Diabetic Retinopathy." Acta Ophthalmologica Scandinavica, vol. 75, no. 4, 1997, pp. 388-92.
Bek T. Immunohistochemical characterization of retinal glial cell changes in areas of vascular occlusion secondary to diabetic retinopathy. Acta Ophthalmol Scand. 1997;75(4):388-92.
Bek, T. (1997). Immunohistochemical characterization of retinal glial cell changes in areas of vascular occlusion secondary to diabetic retinopathy. Acta Ophthalmologica Scandinavica, 75(4), 388-92.
Bek T. Immunohistochemical Characterization of Retinal Glial Cell Changes in Areas of Vascular Occlusion Secondary to Diabetic Retinopathy. Acta Ophthalmol Scand. 1997;75(4):388-92. PubMed PMID: 9374245.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunohistochemical characterization of retinal glial cell changes in areas of vascular occlusion secondary to diabetic retinopathy. A1 - Bek,T, PY - 1997/8/1/pubmed PY - 1997/11/28/medline PY - 1997/8/1/entrez SP - 388 EP - 92 JF - Acta ophthalmologica Scandinavica JO - Acta Ophthalmol Scand VL - 75 IS - 4 N2 - PURPOSE: To study changes in retinal glial cell components in areas of vascular occlusion secondary to diabetic retinopathy. MATERIAL: The retina from ten eyes of six diabetic patients and from five eyes of five normal controls were studied for immunoreactivity to glial fibrillary acid protein and vimentin (glial cells), S-100 protein (perivascular glial cells), carbonic anhydrase isoenzyme II and CD-57 antigen (Müller cells), and CD-68 antigen (microglia). RESULTS: The study showed increased immunoreactivity to S-100 protein, corresponding to perivascularly located glial cells in the retina from diabetic patients, except for areas of vascular occlusion where this immunoreactivity was absent. Furthermore, the material invading the lumen of former retinal vessels in areas of vascular occlusion showed immunoreactivity to CAH-II and CD-57, suggesting that this material represents ingrowth of retinal Müller cells. CONCLUSIONS: The findings suggest that at least two types of changes in retinal glial cells are involved in the pathophysiology of diabetic retinopathy, i.e. 1) Reactive changes in the perivascular glial cells in the retina, and 2) Müller cell ingrowth into the former lumen of occluded retinal vessels. SN - 1395-3907 UR - https://www.unboundmedicine.com/medline/citation/9374245/Immunohistochemical_characterization_of_retinal_glial_cell_changes_in_areas_of_vascular_occlusion_secondary_to_diabetic_retinopathy_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=1395-3907&date=1997&volume=75&issue=4&spage=388 DB - PRIME DP - Unbound Medicine ER -