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A new in vitro assay for quantitation of chemotherapy-induced mucositis.
Br J Cancer. 1997; 76(8):1062-6.BJ

Abstract

Patients receiving high-dose chemotherapy (HD-CT) are at risk of severe mucositis. Most prevention studies evaluate the degree of mucositis on clinical, and therefore subjective, measurements. The aim of this study was to develop an objective in vitro assay of chemotherapy-induced mucositis. Twelve patients with locally advanced breast carcinoma received HD-CT followed by peripheral stem cell reinfusion. Before and twice weekly after HD-CT, the mucosa was evaluated by an oral washing, a buccal smear and the World Health Organization (WHO) toxicity grading; furthermore, blood leucocyte levels were determined. For the oral washings, the percentage of viable epithelial cells was determined by trypan blue dye exclusion and leucocytes were counted by fluorescence microscopy after incubation with acridine orange. Maturity of buccal cells was assessed by staining buccal smears for morphology according to Papanicolaou (Whitacker D and Williams V, 1994). Eight healthy volunteers served as controls. The mean percentage (+/- s.e.m.) of viable oral epithelial cells was stable in controls (44 +/- 2%). In patients, they increased after HD-CT, which was significant after day 7 compared with pretreatment (P < or = 0.05). In addition, a shift from mature to immature epithelial cells in buccal smears was observed. Oral leucocyte levels were closely correlated with the blood leucocyte counts. The WHO score followed the results of these other evaluations with some delay. The viability of buccal cells obtained by oral washings increases after HD-CT. This is possibly because of desquamation of the upper oral mucosa layer, with a shift from mature to more immature cells. These data can be quantitated, and this assay may therefore be useful in studies aimed at prevention of mucositis.

Authors+Show Affiliations

Department of Internal Medicine, University Hospital Groningen, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article

Language

eng

PubMed ID

9376267

Citation

Wymenga, A N., et al. "A New in Vitro Assay for Quantitation of Chemotherapy-induced Mucositis." British Journal of Cancer, vol. 76, no. 8, 1997, pp. 1062-6.
Wymenga AN, van der Graaf WT, Spijkervet FL, et al. A new in vitro assay for quantitation of chemotherapy-induced mucositis. Br J Cancer. 1997;76(8):1062-6.
Wymenga, A. N., van der Graaf, W. T., Spijkervet, F. L., Timens, W., Timmer-Bosscha, H., Sluiter, W. J., de Vries, E. G., & Mulder, N. H. (1997). A new in vitro assay for quantitation of chemotherapy-induced mucositis. British Journal of Cancer, 76(8), 1062-6.
Wymenga AN, et al. A New in Vitro Assay for Quantitation of Chemotherapy-induced Mucositis. Br J Cancer. 1997;76(8):1062-6. PubMed PMID: 9376267.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A new in vitro assay for quantitation of chemotherapy-induced mucositis. AU - Wymenga,A N, AU - van der Graaf,W T, AU - Spijkervet,F L, AU - Timens,W, AU - Timmer-Bosscha,H, AU - Sluiter,W J, AU - de Vries,E G, AU - Mulder,N H, PY - 1997/1/1/pubmed PY - 1997/11/4/medline PY - 1997/1/1/entrez SP - 1062 EP - 6 JF - British journal of cancer JO - Br J Cancer VL - 76 IS - 8 N2 - Patients receiving high-dose chemotherapy (HD-CT) are at risk of severe mucositis. Most prevention studies evaluate the degree of mucositis on clinical, and therefore subjective, measurements. The aim of this study was to develop an objective in vitro assay of chemotherapy-induced mucositis. Twelve patients with locally advanced breast carcinoma received HD-CT followed by peripheral stem cell reinfusion. Before and twice weekly after HD-CT, the mucosa was evaluated by an oral washing, a buccal smear and the World Health Organization (WHO) toxicity grading; furthermore, blood leucocyte levels were determined. For the oral washings, the percentage of viable epithelial cells was determined by trypan blue dye exclusion and leucocytes were counted by fluorescence microscopy after incubation with acridine orange. Maturity of buccal cells was assessed by staining buccal smears for morphology according to Papanicolaou (Whitacker D and Williams V, 1994). Eight healthy volunteers served as controls. The mean percentage (+/- s.e.m.) of viable oral epithelial cells was stable in controls (44 +/- 2%). In patients, they increased after HD-CT, which was significant after day 7 compared with pretreatment (P < or = 0.05). In addition, a shift from mature to immature epithelial cells in buccal smears was observed. Oral leucocyte levels were closely correlated with the blood leucocyte counts. The WHO score followed the results of these other evaluations with some delay. The viability of buccal cells obtained by oral washings increases after HD-CT. This is possibly because of desquamation of the upper oral mucosa layer, with a shift from mature to more immature cells. These data can be quantitated, and this assay may therefore be useful in studies aimed at prevention of mucositis. SN - 0007-0920 UR - https://www.unboundmedicine.com/medline/citation/9376267/A_new_in_vitro_assay_for_quantitation_of_chemotherapy_induced_mucositis_ L2 - https://doi.org/10.1038/bjc.1997.508 DB - PRIME DP - Unbound Medicine ER -