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Myosin heavy chain composition of regenerated soleus muscles during hindlimb suspension.
Acta Physiol Scand. 1997 Sep; 161(1):23-30.AP

Abstract

The aim of this study was to determine the MHC profile of regenerated soleus muscles in control (C, n = 8) and hindlimb suspended rats (HS, n = 8). After muscle degeneration was induced by injection of snake venom containing notexin, male rats were either tail suspended for 21 days or submitted to normal weight-bearing activity. Separation and detection of MHCs by sodium dodecyl sulphate-polyarcylamide gel electrophoresis (SDS-PAGE) showed that regenerated soleus muscles from C rats contained only type I and type IIa MHCs. The relative amount of type I MHC was higher in regenerated (93.9 +/- 1.7%) than in untreated muscles (86.5 +/- 2.3%)(P < 0.01). In the HS group, the immunohistochemical analysis showed that the majority of regenerated myofibres reacted positively with the antibody against fast MHCs. SDS-PAGE analysis demonstrated that HS resulted in a shift toward faster MHCs in both intact and regenerated myofibres. Regenerated soleus muscle from HS rats contained approximately 34% type IIa MHC, approximately 37% type IIx/d MHC and approximately 18% type IIb MHC, when type I MHC contributed to only approximately 12% of total myosin. The proportions of fast MHC isoforms in regenerated muscles were higher than those recorded in untreated muscles. Collectively, these results suggest that the shift in the MHC profile associated with hindlimb unweighting in adult undamaged soleus muscles is also related to the heterogeneity of early myoblasts.

Authors+Show Affiliations

Unité de Bioénergétique et Environnement, Centre de Recherches du Service de Santé des Armées, La Tronche, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9381946

Citation

Bigard, A X., et al. "Myosin Heavy Chain Composition of Regenerated Soleus Muscles During Hindlimb Suspension." Acta Physiologica Scandinavica, vol. 161, no. 1, 1997, pp. 23-30.
Bigard AX, Serrurier B, Merino D, et al. Myosin heavy chain composition of regenerated soleus muscles during hindlimb suspension. Acta Physiol Scand. 1997;161(1):23-30.
Bigard, A. X., Serrurier, B., Merino, D., Lienhard, F., Berthelot, M., & Guezennec, C. Y. (1997). Myosin heavy chain composition of regenerated soleus muscles during hindlimb suspension. Acta Physiologica Scandinavica, 161(1), 23-30.
Bigard AX, et al. Myosin Heavy Chain Composition of Regenerated Soleus Muscles During Hindlimb Suspension. Acta Physiol Scand. 1997;161(1):23-30. PubMed PMID: 9381946.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Myosin heavy chain composition of regenerated soleus muscles during hindlimb suspension. AU - Bigard,A X, AU - Serrurier,B, AU - Merino,D, AU - Lienhard,F, AU - Berthelot,M, AU - Guezennec,C Y, PY - 1997/10/6/pubmed PY - 1997/10/6/medline PY - 1997/10/6/entrez SP - 23 EP - 30 JF - Acta physiologica Scandinavica JO - Acta Physiol Scand VL - 161 IS - 1 N2 - The aim of this study was to determine the MHC profile of regenerated soleus muscles in control (C, n = 8) and hindlimb suspended rats (HS, n = 8). After muscle degeneration was induced by injection of snake venom containing notexin, male rats were either tail suspended for 21 days or submitted to normal weight-bearing activity. Separation and detection of MHCs by sodium dodecyl sulphate-polyarcylamide gel electrophoresis (SDS-PAGE) showed that regenerated soleus muscles from C rats contained only type I and type IIa MHCs. The relative amount of type I MHC was higher in regenerated (93.9 +/- 1.7%) than in untreated muscles (86.5 +/- 2.3%)(P < 0.01). In the HS group, the immunohistochemical analysis showed that the majority of regenerated myofibres reacted positively with the antibody against fast MHCs. SDS-PAGE analysis demonstrated that HS resulted in a shift toward faster MHCs in both intact and regenerated myofibres. Regenerated soleus muscle from HS rats contained approximately 34% type IIa MHC, approximately 37% type IIx/d MHC and approximately 18% type IIb MHC, when type I MHC contributed to only approximately 12% of total myosin. The proportions of fast MHC isoforms in regenerated muscles were higher than those recorded in untreated muscles. Collectively, these results suggest that the shift in the MHC profile associated with hindlimb unweighting in adult undamaged soleus muscles is also related to the heterogeneity of early myoblasts. SN - 0001-6772 UR - https://www.unboundmedicine.com/medline/citation/9381946/Myosin_heavy_chain_composition_of_regenerated_soleus_muscles_during_hindlimb_suspension_ L2 - https://doi.org/10.1046/j.1365-201X.1997.00193.x DB - PRIME DP - Unbound Medicine ER -