Tags

Type your tag names separated by a space and hit enter

Long-term treatment of indomethacin reduces vertebral bone mass and strength in ovariectomized rats.
J Bone Miner Res. 1997 Nov; 12(11):1844-50.JB

Abstract

We investigated the effect of the long-term treatment of indomethacin, on lumbar spinal bone mineral density (LSBMD), cancellous bone mass, structure, and strength of vertebral body in old ovariectomized (OVX) rats. Ten-month-old female Wistar rats were divided into five groups: the sham operated + vehicle (sham + VEH) group, the OVX + vehicle (OVX + VEH) group, the OVX + indomethacin (IN) 1.5 mg/kg/week (OVX + IN1.5) group, the OVX + IN 6.0 mg/kg/week (OVX + IN6.0) group, and the OVX + IN 15.0 mg/kg/week (OVX + IN15.0) group. IN or vehicle were given by subcutaneous injection (s.c.) three times per week. The treatments were started at 1 week after operation and continued for 24 weeks. LSBMD (L2-L5) was measured at 0, 12, and 24 weeks after the beginning of treatment. At the end of the experimental period, the animals were sacrificed, and bone histomorphometrical and biomechanical analysis of lumbar vertebral body were done. LSBMD, trabecular bone volume (BV/TV), and trabecular thickness (Tb.Th) decreased significantly in a dose-related manner with IN. In the OVX + IN15.0 group, LSBMD decreased by 12.7%, BV/TV decreased by 65.5%, and Tb.Th decreased by 32.8%, compared with the OVX + VEH group. In addition, the maximum stress in a compressive mechanical test of L4 vertebral body in OVX groups was also decreased in a dose-related manner with IN, and this value in the OVX + IN15.0 group was 31.3% lower than in the OVX + VEH group. We conclude that long-term treatment with IN accentuated the OVX-related decrease in trabecular bone mass and the compressive strength of lumbar vertebrae.

Authors+Show Affiliations

Department of Orthopaedic Surgery, Sapporo Medical University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9383689

Citation

Saino, H, et al. "Long-term Treatment of Indomethacin Reduces Vertebral Bone Mass and Strength in Ovariectomized Rats." Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research, vol. 12, no. 11, 1997, pp. 1844-50.
Saino H, Matsuyama T, Takada J, et al. Long-term treatment of indomethacin reduces vertebral bone mass and strength in ovariectomized rats. J Bone Miner Res. 1997;12(11):1844-50.
Saino, H., Matsuyama, T., Takada, J., Kaku, T., & Ishii, S. (1997). Long-term treatment of indomethacin reduces vertebral bone mass and strength in ovariectomized rats. Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research, 12(11), 1844-50.
Saino H, et al. Long-term Treatment of Indomethacin Reduces Vertebral Bone Mass and Strength in Ovariectomized Rats. J Bone Miner Res. 1997;12(11):1844-50. PubMed PMID: 9383689.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Long-term treatment of indomethacin reduces vertebral bone mass and strength in ovariectomized rats. AU - Saino,H, AU - Matsuyama,T, AU - Takada,J, AU - Kaku,T, AU - Ishii,S, PY - 1998/2/7/pubmed PY - 1998/2/7/medline PY - 1998/2/7/entrez SP - 1844 EP - 50 JF - Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research JO - J Bone Miner Res VL - 12 IS - 11 N2 - We investigated the effect of the long-term treatment of indomethacin, on lumbar spinal bone mineral density (LSBMD), cancellous bone mass, structure, and strength of vertebral body in old ovariectomized (OVX) rats. Ten-month-old female Wistar rats were divided into five groups: the sham operated + vehicle (sham + VEH) group, the OVX + vehicle (OVX + VEH) group, the OVX + indomethacin (IN) 1.5 mg/kg/week (OVX + IN1.5) group, the OVX + IN 6.0 mg/kg/week (OVX + IN6.0) group, and the OVX + IN 15.0 mg/kg/week (OVX + IN15.0) group. IN or vehicle were given by subcutaneous injection (s.c.) three times per week. The treatments were started at 1 week after operation and continued for 24 weeks. LSBMD (L2-L5) was measured at 0, 12, and 24 weeks after the beginning of treatment. At the end of the experimental period, the animals were sacrificed, and bone histomorphometrical and biomechanical analysis of lumbar vertebral body were done. LSBMD, trabecular bone volume (BV/TV), and trabecular thickness (Tb.Th) decreased significantly in a dose-related manner with IN. In the OVX + IN15.0 group, LSBMD decreased by 12.7%, BV/TV decreased by 65.5%, and Tb.Th decreased by 32.8%, compared with the OVX + VEH group. In addition, the maximum stress in a compressive mechanical test of L4 vertebral body in OVX groups was also decreased in a dose-related manner with IN, and this value in the OVX + IN15.0 group was 31.3% lower than in the OVX + VEH group. We conclude that long-term treatment with IN accentuated the OVX-related decrease in trabecular bone mass and the compressive strength of lumbar vertebrae. SN - 0884-0431 UR - https://www.unboundmedicine.com/medline/citation/9383689/Long_term_treatment_of_indomethacin_reduces_vertebral_bone_mass_and_strength_in_ovariectomized_rats_ DB - PRIME DP - Unbound Medicine ER -