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Influence of Epstein-Barr virus latent gene expression on the apoptosis-inducing effects of cortisone and 2-chlorodeoxyadenosine (2-CDA) in B-cell lines.
Cytokines Mol Ther 1996; 2(1):21-8CM

Abstract

Burkitt's lymphoma (BL) cell lines are heterogenous with regard to phenotype, growth characteristics, Epstein-Barr virus (EBV) latent gene and BCL-2 expression. Previously we have demonstrated that transfection with the EBV genes LMP or EBNA-2 upregulates BCL-2 in B-cell lines. In order to test the functional relevance of these findings, cell lines were examined with regard to their sensitivity towards different apoptosis-inducing agents. BL cell lines transfected with LMP expressed high levels of BCL-2, and were compared with the parental cell line expressing little or no BCL-2. We also studied EBV immortalized lymphoblastoid cell lines (LCL) with high BCL-2 expression and strong resistance towards low serum concentrations. Hydrocortisone (HC) and 2-chlorodeoxyadenosine (2-CDA) were used alone or in different combinations. Cell growth and apoptosis were studied morphologically and by determination of viability and DNA fragmentation. BL cell lines showed different sensitivity towards HC-induced apoptosis, and sensitive cell lines became more resistant towards HC after infection with EBV or transfection with LMP and subsequent upregulation of BCL-2 expression. BL cell lines and LCL were relatively insensitive towards 2-CDA-induced apoptosis, and high concentrations of 2-CDA were necessary, independently of the levels of BCL-2 expression. In contrast to low-grade non-Hodgkin's lymphomas, 2-CDA does not appear to be a valuable drug for the treatment of Burkitt's lymphoma. LMP expression provides resistance towards hydrocortisone-induced apoptosis in vitro, possible through upregulation of BCL-2.

Authors+Show Affiliations

Department of Hematology and Oncology, University of Freiburg Medical Center, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9384686

Citation

Röth, A, et al. "Influence of Epstein-Barr Virus Latent Gene Expression On the Apoptosis-inducing Effects of Cortisone and 2-chlorodeoxyadenosine (2-CDA) in B-cell Lines." Cytokines and Molecular Therapy, vol. 2, no. 1, 1996, pp. 21-8.
Röth A, Pfaff P, Lange W, et al. Influence of Epstein-Barr virus latent gene expression on the apoptosis-inducing effects of cortisone and 2-chlorodeoxyadenosine (2-CDA) in B-cell lines. Cytokines Mol Ther. 1996;2(1):21-8.
Röth, A., Pfaff, P., Lange, W., & Finke, J. (1996). Influence of Epstein-Barr virus latent gene expression on the apoptosis-inducing effects of cortisone and 2-chlorodeoxyadenosine (2-CDA) in B-cell lines. Cytokines and Molecular Therapy, 2(1), pp. 21-8.
Röth A, et al. Influence of Epstein-Barr Virus Latent Gene Expression On the Apoptosis-inducing Effects of Cortisone and 2-chlorodeoxyadenosine (2-CDA) in B-cell Lines. Cytokines Mol Ther. 1996;2(1):21-8. PubMed PMID: 9384686.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of Epstein-Barr virus latent gene expression on the apoptosis-inducing effects of cortisone and 2-chlorodeoxyadenosine (2-CDA) in B-cell lines. AU - Röth,A, AU - Pfaff,P, AU - Lange,W, AU - Finke,J, PY - 1996/3/1/pubmed PY - 1997/12/31/medline PY - 1996/3/1/entrez SP - 21 EP - 8 JF - Cytokines and molecular therapy JO - Cytokines Mol Ther VL - 2 IS - 1 N2 - Burkitt's lymphoma (BL) cell lines are heterogenous with regard to phenotype, growth characteristics, Epstein-Barr virus (EBV) latent gene and BCL-2 expression. Previously we have demonstrated that transfection with the EBV genes LMP or EBNA-2 upregulates BCL-2 in B-cell lines. In order to test the functional relevance of these findings, cell lines were examined with regard to their sensitivity towards different apoptosis-inducing agents. BL cell lines transfected with LMP expressed high levels of BCL-2, and were compared with the parental cell line expressing little or no BCL-2. We also studied EBV immortalized lymphoblastoid cell lines (LCL) with high BCL-2 expression and strong resistance towards low serum concentrations. Hydrocortisone (HC) and 2-chlorodeoxyadenosine (2-CDA) were used alone or in different combinations. Cell growth and apoptosis were studied morphologically and by determination of viability and DNA fragmentation. BL cell lines showed different sensitivity towards HC-induced apoptosis, and sensitive cell lines became more resistant towards HC after infection with EBV or transfection with LMP and subsequent upregulation of BCL-2 expression. BL cell lines and LCL were relatively insensitive towards 2-CDA-induced apoptosis, and high concentrations of 2-CDA were necessary, independently of the levels of BCL-2 expression. In contrast to low-grade non-Hodgkin's lymphomas, 2-CDA does not appear to be a valuable drug for the treatment of Burkitt's lymphoma. LMP expression provides resistance towards hydrocortisone-induced apoptosis in vitro, possible through upregulation of BCL-2. SN - 1355-6568 UR - https://www.unboundmedicine.com/medline/citation/9384686/Influence_of_Epstein_Barr_virus_latent_gene_expression_on_the_apoptosis_inducing_effects_of_cortisone_and_2_chlorodeoxyadenosine__2_CDA__in_B_cell_lines_ DB - PRIME DP - Unbound Medicine ER -