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Capillary electrophoretic behavior of seven sulfonylureas.
J Capillary Electrophor. 1996 Jul-Aug; 3(4):199-204.JC

Abstract

The electrophoretic behavior of seven sulfonylureas (bensulfuron methyl, sulfometuron methyl, nicosulfuron [accent], chlorimuron ethyl, thifensulfuron methyl [harmony], metsulfuron methyl, and chlorsulfuron) was studied under capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) conditions. Mixtures of these compounds were separated with very high efficiencies (2 x 10(5) theoretical plates) in a running buffer consisting of 3 parts acetate buffer (25 mM, pH 5.0) and 1 part acetonitrile. In this buffer system, acetonitrile was shown to be superior to methanol, acetone, and ethanol as a nonpolar additive, but any of these solvents can be used to reduce electroosmotic flow (EOF) and to obtain adequate separation. On-column detection limits at 214 nM were of the order of 80-100 fM. Micellar agents such as sodium dodecyl sulfate (SDS) and sodium cholate (but not monosialoganglioside-Gm1 or starburst dendrimer, generation 2.5) improved separation in phosphate and borate buffers. Implications of these results for the development of methods to detect these compounds on matrices of environmental origin are discussed. In particular, the instability of these compounds in methanol is noted and degradation products are detected using free zone CE. The methanolysis products of sulfometuron are tentatively identified by tandem MS (negative ion conditions) as 2-amino-4,6-dimethylpyrimidine and 2-carboxymethylbenz(N-carboxymethyl)sulfonamide.

Authors+Show Affiliations

U.S. Environmental Protection Agency, National Exposure Research Laboratory, Las Vegas, NV 89193-3478, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9384737

Citation

Matchett, W H., et al. "Capillary Electrophoretic Behavior of Seven Sulfonylureas." Journal of Capillary Electrophoresis, vol. 3, no. 4, 1996, pp. 199-204.
Matchett WH, Winnik W, Brumley WC. Capillary electrophoretic behavior of seven sulfonylureas. J Capillary Electrophor. 1996;3(4):199-204.
Matchett, W. H., Winnik, W., & Brumley, W. C. (1996). Capillary electrophoretic behavior of seven sulfonylureas. Journal of Capillary Electrophoresis, 3(4), 199-204.
Matchett WH, Winnik W, Brumley WC. Capillary Electrophoretic Behavior of Seven Sulfonylureas. J Capillary Electrophor. 1996 Jul-Aug;3(4):199-204. PubMed PMID: 9384737.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Capillary electrophoretic behavior of seven sulfonylureas. AU - Matchett,W H, AU - Winnik,W, AU - Brumley,W C, PY - 1996/7/1/pubmed PY - 1997/12/31/medline PY - 1996/7/1/entrez SP - 199 EP - 204 JF - Journal of capillary electrophoresis JO - J Capillary Electrophor VL - 3 IS - 4 N2 - The electrophoretic behavior of seven sulfonylureas (bensulfuron methyl, sulfometuron methyl, nicosulfuron [accent], chlorimuron ethyl, thifensulfuron methyl [harmony], metsulfuron methyl, and chlorsulfuron) was studied under capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) conditions. Mixtures of these compounds were separated with very high efficiencies (2 x 10(5) theoretical plates) in a running buffer consisting of 3 parts acetate buffer (25 mM, pH 5.0) and 1 part acetonitrile. In this buffer system, acetonitrile was shown to be superior to methanol, acetone, and ethanol as a nonpolar additive, but any of these solvents can be used to reduce electroosmotic flow (EOF) and to obtain adequate separation. On-column detection limits at 214 nM were of the order of 80-100 fM. Micellar agents such as sodium dodecyl sulfate (SDS) and sodium cholate (but not monosialoganglioside-Gm1 or starburst dendrimer, generation 2.5) improved separation in phosphate and borate buffers. Implications of these results for the development of methods to detect these compounds on matrices of environmental origin are discussed. In particular, the instability of these compounds in methanol is noted and degradation products are detected using free zone CE. The methanolysis products of sulfometuron are tentatively identified by tandem MS (negative ion conditions) as 2-amino-4,6-dimethylpyrimidine and 2-carboxymethylbenz(N-carboxymethyl)sulfonamide. SN - 1079-5383 UR - https://www.unboundmedicine.com/medline/citation/9384737/Capillary_electrophoretic_behavior_of_seven_sulfonylureas_ DB - PRIME DP - Unbound Medicine ER -