Systematic development of on-line membrane preconcentration-capillary electrophoresis-mass spectrometry for the analysis of peptide mixtures.
J Capillary Electrophor. 1995 Sep-Oct; 2(5):225-33.JC

Abstract

A unique method for analyte preconcentration on-line with CE and CE-mass spectrometry (CE-MS) that can concentrate large sample volumes (> 100 microL) is described. A cartridge containing a suitably impregnated membrane is installed at the inlet of the CE capillary, and this approach is termed membrane preconcentration-CE-MS (mPC-CE-MS). The authors describe the mPC-CE-MS analysis of dilute peptide mixtures (approximately 1 fmol/nL) that are concentrated onto the membrane and subsequently subjected to on-line sample cleanup prior to elution and ultimately CE-MS. The systematic development of mPC-CE-MS is demonstrated, and the necessity of minimizing the adsorptive phase in order to ensure efficient peptide analyte recovery and minimization of organic elution solvent is described. The authors compare and contrast mPC-CE and mPC-CE-MS with PC-CE and solid-phase preconcentration-CE-MS (PC-CE-MS), and describe several limitations of the latter approach, which includes analyte zone broadening and compromised analyte resolution. Finally, the authors show that the use of mPC-CE-MS in conjunction with transient isotachophoresis after the peptides have been eluted from the adsorptive membrane affords optimal performance, and theoretical plate values of up to approximately 2.6 x 10(6) are observed for the analysis of a nine-component peptide mixture using this approach. It is conservatively estimated that, in conjunction with an MS array detector, a practical concentration limit of detection (CLOD) for peptides is approximately 500 fg-1 pg/mL using mPC-CE-MS.

Authors+Show Affiliations

Tomlinson AJ

Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester MN 55905, USA.

Naylor S

No affiliation info available

MeSH

Electrophoresis, CapillaryMass SpectrometryPeptides

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9384780

Citation

Tomlinson, A J., and S Naylor. "Systematic Development of On-line Membrane Preconcentration-capillary Electrophoresis-mass Spectrometry for the Analysis of Peptide Mixtures." Journal of Capillary Electrophoresis, vol. 2, no. 5, 1995, pp. 225-33.

Tomlinson AJ, Naylor S. Systematic development of on-line membrane preconcentration-capillary electrophoresis-mass spectrometry for the analysis of peptide mixtures. J Capillary Electrophor. 1995;2(5):225-33.

Tomlinson, A. J., & Naylor, S. (1995). Systematic development of on-line membrane preconcentration-capillary electrophoresis-mass spectrometry for the analysis of peptide mixtures. Journal of Capillary Electrophoresis, 2(5), 225-33.

Tomlinson AJ, Naylor S. Systematic Development of On-line Membrane Preconcentration-capillary Electrophoresis-mass Spectrometry for the Analysis of Peptide Mixtures. J Capillary Electrophor. 1995 Sep-Oct;2(5):225-33. PubMed PMID: 9384780.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Systematic development of on-line membrane preconcentration-capillary electrophoresis-mass spectrometry for the analysis of peptide mixtures. AU - Tomlinson,A J, AU - Naylor,S, PY - 1995/9/1/pubmed PY - 1997/12/31/medline PY - 1995/9/1/entrez SP - 225 EP - 33 JF - Journal of capillary electrophoresis JO - J Capillary Electrophor VL - 2 IS - 5 N2 - A unique method for analyte preconcentration on-line with CE and CE-mass spectrometry (CE-MS) that can concentrate large sample volumes (> 100 microL) is described. A cartridge containing a suitably impregnated membrane is installed at the inlet of the CE capillary, and this approach is termed membrane preconcentration-CE-MS (mPC-CE-MS). The authors describe the mPC-CE-MS analysis of dilute peptide mixtures (approximately 1 fmol/nL) that are concentrated onto the membrane and subsequently subjected to on-line sample cleanup prior to elution and ultimately CE-MS. The systematic development of mPC-CE-MS is demonstrated, and the necessity of minimizing the adsorptive phase in order to ensure efficient peptide analyte recovery and minimization of organic elution solvent is described. The authors compare and contrast mPC-CE and mPC-CE-MS with PC-CE and solid-phase preconcentration-CE-MS (PC-CE-MS), and describe several limitations of the latter approach, which includes analyte zone broadening and compromised analyte resolution. Finally, the authors show that the use of mPC-CE-MS in conjunction with transient isotachophoresis after the peptides have been eluted from the adsorptive membrane affords optimal performance, and theoretical plate values of up to approximately 2.6 x 10(6) are observed for the analysis of a nine-component peptide mixture using this approach. It is conservatively estimated that, in conjunction with an MS array detector, a practical concentration limit of detection (CLOD) for peptides is approximately 500 fg-1 pg/mL using mPC-CE-MS. SN - 1079-5383 UR - https://www.unboundmedicine.com/medline/citation/9384780/Systematic_development_of_on_line_membrane_preconcentration_capillary_electrophoresis_mass_spectrometry_for_the_analysis_of_peptide_mixtures_ L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:9384780 DB - PRIME DP - Unbound Medicine ER -

Tags

Systematic development of on-line membrane preconcentration-capillary electrophoresis-mass spectrometry for the analysis of peptide mixtures.J Capillary Electrophor. 1995 Sep-Oct; 2(5):225-33.JC