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Aqueous alkylchloroformate derivatisation and solid-phase microextraction: determination of amphetamines in urine by capillary gas chromatography.
J Chromatogr B Biomed Sci Appl. 1997 Nov 07; 701(1):29-38.JC

Abstract

The alkylchloroformate derivatisation and solid-phase microextraction of amphetamine and methamphetamine directly in urine samples prior to capillary gas chromatographic analysis is described. The alkylchloroformate reagent was added to the urine sample, which was adjusted to pH 10.8, and an internal standard was added. The resulting products were water-stable carbamates that were extracted without organic solvent. The polydimethylsiloxane coated fibre was inserted into the modified sample and agitated for 14 min. The fibre with the extracted derivatisation products was injected into the capillary gas chromatograph. The extracted carbamates were evaporated at 300 degrees C in the split-splitless injection port of the gas chromatograph, separated on a methylsilicone capillary column and detected by either a nitrogen-phosphorus detector or by mass spectrometry. The method was shown to be reproducible with a detection limit of 50 ng/ml of amphetamine and methamphetamine in urine.

Authors+Show Affiliations

Institute of Pharmacy, University of Oslo, Blindern, Norway.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9389335

Citation

Ugland, H G., et al. "Aqueous Alkylchloroformate Derivatisation and Solid-phase Microextraction: Determination of Amphetamines in Urine By Capillary Gas Chromatography." Journal of Chromatography. B, Biomedical Sciences and Applications, vol. 701, no. 1, 1997, pp. 29-38.
Ugland HG, Krogh M, Rasmussen KE. Aqueous alkylchloroformate derivatisation and solid-phase microextraction: determination of amphetamines in urine by capillary gas chromatography. J Chromatogr B Biomed Sci Appl. 1997;701(1):29-38.
Ugland, H. G., Krogh, M., & Rasmussen, K. E. (1997). Aqueous alkylchloroformate derivatisation and solid-phase microextraction: determination of amphetamines in urine by capillary gas chromatography. Journal of Chromatography. B, Biomedical Sciences and Applications, 701(1), 29-38.
Ugland HG, Krogh M, Rasmussen KE. Aqueous Alkylchloroformate Derivatisation and Solid-phase Microextraction: Determination of Amphetamines in Urine By Capillary Gas Chromatography. J Chromatogr B Biomed Sci Appl. 1997 Nov 7;701(1):29-38. PubMed PMID: 9389335.
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TY - JOUR T1 - Aqueous alkylchloroformate derivatisation and solid-phase microextraction: determination of amphetamines in urine by capillary gas chromatography. AU - Ugland,H G, AU - Krogh,M, AU - Rasmussen,K E, PY - 1997/12/6/pubmed PY - 1997/12/6/medline PY - 1997/12/6/entrez SP - 29 EP - 38 JF - Journal of chromatography. B, Biomedical sciences and applications JO - J Chromatogr B Biomed Sci Appl VL - 701 IS - 1 N2 - The alkylchloroformate derivatisation and solid-phase microextraction of amphetamine and methamphetamine directly in urine samples prior to capillary gas chromatographic analysis is described. The alkylchloroformate reagent was added to the urine sample, which was adjusted to pH 10.8, and an internal standard was added. The resulting products were water-stable carbamates that were extracted without organic solvent. The polydimethylsiloxane coated fibre was inserted into the modified sample and agitated for 14 min. The fibre with the extracted derivatisation products was injected into the capillary gas chromatograph. The extracted carbamates were evaporated at 300 degrees C in the split-splitless injection port of the gas chromatograph, separated on a methylsilicone capillary column and detected by either a nitrogen-phosphorus detector or by mass spectrometry. The method was shown to be reproducible with a detection limit of 50 ng/ml of amphetamine and methamphetamine in urine. SN - 1387-2273 UR - https://www.unboundmedicine.com/medline/citation/9389335/Aqueous_alkylchloroformate_derivatisation_and_solid_phase_microextraction:_determination_of_amphetamines_in_urine_by_capillary_gas_chromatography_ L2 - https://medlineplus.gov/methamphetamine.html DB - PRIME DP - Unbound Medicine ER -