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A null mutation in the Bacillus subtilis aconitase gene causes a block in Spo0A-phosphate-dependent gene expression.
J Bacteriol. 1997 Dec; 179(23):7351-9.JB

Abstract

The citB gene of Bacillus subtilis encodes aconitase, the enzyme of the Krebs citric acid cycle, which is responsible for the interconversion of citrate and isocitrate. A B. subtilis strain with an insertion mutation in the citB gene was devoid of aconitase activity and aconitase protein, required glutamate for growth in minimal medium, and was unable to sporulate efficiently in nutrient broth sporulation medium. Mutant cells failed to form the asymmetric septum characteristic of sporulating cells and were defective in transcription of the earliest-expressed spo genes, that is, the genes dependent on the Spo0A phosphorelay. However, this early block in sporulation was partially overcome when cells of the citB mutant were induced to sporulate by resuspension in a poor medium. Accumulation of citrate in the mutant cells or in their culture fluid may be responsible for the early block, possibly because citrate can chelate divalent cations needed for the activity of the phosphorelay.

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Authors+Show Affiliations

Craig JE
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
Ford MJ
No affiliation info available
Blaydon DC
No affiliation info available
Sonenshein AL
No affiliation info available

MeSH

Aconitate HydrataseBacillus subtilisBacterial ProteinsCitrate (si)-SynthaseGene Expression Regulation, BacterialMutationPhenotypePhosphatesSignal TransductionSpores, BacterialTranscription Factors

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9393699

Citation

Craig, J E., et al. "A Null Mutation in the Bacillus Subtilis Aconitase Gene Causes a Block in Spo0A-phosphate-dependent Gene Expression." Journal of Bacteriology, vol. 179, no. 23, 1997, pp. 7351-9.
Craig JE, Ford MJ, Blaydon DC, et al. A null mutation in the Bacillus subtilis aconitase gene causes a block in Spo0A-phosphate-dependent gene expression. J Bacteriol. 1997;179(23):7351-9.
Craig, J. E., Ford, M. J., Blaydon, D. C., & Sonenshein, A. L. (1997). A null mutation in the Bacillus subtilis aconitase gene causes a block in Spo0A-phosphate-dependent gene expression. Journal of Bacteriology, 179(23), 7351-9.
Craig JE, et al. A Null Mutation in the Bacillus Subtilis Aconitase Gene Causes a Block in Spo0A-phosphate-dependent Gene Expression. J Bacteriol. 1997;179(23):7351-9. PubMed PMID: 9393699.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A null mutation in the Bacillus subtilis aconitase gene causes a block in Spo0A-phosphate-dependent gene expression. AU - Craig,J E, AU - Ford,M J, AU - Blaydon,D C, AU - Sonenshein,A L, PY - 1997/12/11/pubmed PY - 1997/12/11/medline PY - 1997/12/11/entrez SP - 7351 EP - 9 JF - Journal of bacteriology JO - J Bacteriol VL - 179 IS - 23 N2 - The citB gene of Bacillus subtilis encodes aconitase, the enzyme of the Krebs citric acid cycle, which is responsible for the interconversion of citrate and isocitrate. A B. subtilis strain with an insertion mutation in the citB gene was devoid of aconitase activity and aconitase protein, required glutamate for growth in minimal medium, and was unable to sporulate efficiently in nutrient broth sporulation medium. Mutant cells failed to form the asymmetric septum characteristic of sporulating cells and were defective in transcription of the earliest-expressed spo genes, that is, the genes dependent on the Spo0A phosphorelay. However, this early block in sporulation was partially overcome when cells of the citB mutant were induced to sporulate by resuspension in a poor medium. Accumulation of citrate in the mutant cells or in their culture fluid may be responsible for the early block, possibly because citrate can chelate divalent cations needed for the activity of the phosphorelay. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/9393699/A_null_mutation_in_the_Bacillus_subtilis_aconitase_gene_causes_a_block_in_Spo0A_phosphate_dependent_gene_expression_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=9393699 DB - PRIME DP - Unbound Medicine ER -