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Dentin sialoprotein (DSP) transcripts: developmentally-sustained expression in odontoblasts and transient expression in pre-ameloblasts.
Eur J Oral Sci 1997; 105(5 Pt 1):405-13EJ

Abstract

Dentin sialoprotein (DSP), a 53 kDa glycoprotein, is believed to be present exclusively in dentin. Using rat and mouse digoxigenin labeled (DIG)-DSP and 35S-DSP riboprobes, and in situ hybridization techniques, we have studied the presence of DSP mRNA at specific developmental stages of dentinogenesis. In mouse and rat molars and incisors, DSP transcripts were localized in young odontoblasts associated with early stages of predentin formation, as well as in mature odontoblasts, cells with cytoplasmic extensions embedded in the forming dentin. No DSP transcripts were detected in dental pulp, enamel organ, ameloblasts, epithelial root sheath, Meckel's cartilage, alveolar bone or tibia. Furthermore, no DSP mRNA was observed in other soft tissues including heart, lung, kidney, intestine, eye, and muscle. In addition to the intense and prolonged expression by odontoblasts, DSP mRNA was transiently expressed by pre-ameloblasts in both developing molars and incisors. These observations are consistent with the results of previous immunohistochemical studies (1). The transient expression of DSP in pre-ameloblasts across from young odontoblasts suggests an involvement of DSP in epithelial-mesenchymal interactions that are crucial to later stages of tooth development.

Authors+Show Affiliations

Department of Basic Sciences, University of Texas-Houston Health Science Center, Dental Branch, 77030, USA. helena-ritchie@uiowa.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9395101

Citation

Ritchie, H H., et al. "Dentin Sialoprotein (DSP) Transcripts: Developmentally-sustained Expression in Odontoblasts and Transient Expression in Pre-ameloblasts." European Journal of Oral Sciences, vol. 105, no. 5 Pt 1, 1997, pp. 405-13.
Ritchie HH, Berry JE, Somerman MJ, et al. Dentin sialoprotein (DSP) transcripts: developmentally-sustained expression in odontoblasts and transient expression in pre-ameloblasts. Eur J Oral Sci. 1997;105(5 Pt 1):405-13.
Ritchie, H. H., Berry, J. E., Somerman, M. J., Hanks, C. T., Bronckers, A. L., Hotton, D., ... Butler, W. T. (1997). Dentin sialoprotein (DSP) transcripts: developmentally-sustained expression in odontoblasts and transient expression in pre-ameloblasts. European Journal of Oral Sciences, 105(5 Pt 1), pp. 405-13.
Ritchie HH, et al. Dentin Sialoprotein (DSP) Transcripts: Developmentally-sustained Expression in Odontoblasts and Transient Expression in Pre-ameloblasts. Eur J Oral Sci. 1997;105(5 Pt 1):405-13. PubMed PMID: 9395101.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dentin sialoprotein (DSP) transcripts: developmentally-sustained expression in odontoblasts and transient expression in pre-ameloblasts. AU - Ritchie,H H, AU - Berry,J E, AU - Somerman,M J, AU - Hanks,C T, AU - Bronckers,A L, AU - Hotton,D, AU - Papagerakis,P, AU - Berdal,A, AU - Butler,W T, PY - 1997/12/12/pubmed PY - 1997/12/12/medline PY - 1997/12/12/entrez SP - 405 EP - 13 JF - European journal of oral sciences JO - Eur. J. Oral Sci. VL - 105 IS - 5 Pt 1 N2 - Dentin sialoprotein (DSP), a 53 kDa glycoprotein, is believed to be present exclusively in dentin. Using rat and mouse digoxigenin labeled (DIG)-DSP and 35S-DSP riboprobes, and in situ hybridization techniques, we have studied the presence of DSP mRNA at specific developmental stages of dentinogenesis. In mouse and rat molars and incisors, DSP transcripts were localized in young odontoblasts associated with early stages of predentin formation, as well as in mature odontoblasts, cells with cytoplasmic extensions embedded in the forming dentin. No DSP transcripts were detected in dental pulp, enamel organ, ameloblasts, epithelial root sheath, Meckel's cartilage, alveolar bone or tibia. Furthermore, no DSP mRNA was observed in other soft tissues including heart, lung, kidney, intestine, eye, and muscle. In addition to the intense and prolonged expression by odontoblasts, DSP mRNA was transiently expressed by pre-ameloblasts in both developing molars and incisors. These observations are consistent with the results of previous immunohistochemical studies (1). The transient expression of DSP in pre-ameloblasts across from young odontoblasts suggests an involvement of DSP in epithelial-mesenchymal interactions that are crucial to later stages of tooth development. SN - 0909-8836 UR - https://www.unboundmedicine.com/medline/citation/9395101/Dentin_sialoprotein__DSP__transcripts:_developmentally_sustained_expression_in_odontoblasts_and_transient_expression_in_pre_ameloblasts_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0909-8836&date=1997&volume=105&issue=5 Pt 1&spage=405 DB - PRIME DP - Unbound Medicine ER -