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Comparison of gelatin particle agglutination and hemagglutination inhibition tests for measles seroepidemiology studies.
Arch Virol. 1997; 142(10):1963-70.AV

Abstract

The prevalence of measles antibody in Japan was surveyed with a newly developed gelatin particle agglutination (PA) test, and the results compared with those of the hemagglutination inhibition (HI) test. The two age-distribution curves of the PA antibody-positive rates at > or = 1:8 and > or = 1:32 were almost the same in all the age groups, except the less-than-1-year-old group for which the rate at > or = 1:8 was higher than that at > or = 1:32 (p < 0.05, chi 2 test). In the vaccinated children, all groups older-than-1-year of age had antibody-positive levels of 96% or more. In contrast, in the unvaccinated children, there was a sharp increase in antibody-positive rates between the 1- and 4-year-old groups, indicative that about 80% of the children were infected by wild measles virus at these ages. A significant number of PA antibody-positive specimens were antibody-negative (< 1:8) by HI. The percentage of specimens in this category, PA (+) but HI (-), was greatest in infants less than one year old, and least in young children, but it increased with age to 97% of the HI (-) specimens from adults of more than 20 years of age. The PA test therefore detected some measles antibodies that HI could not. This test is simple and useful for making serosurveys in both developed and developing countries.

Authors+Show Affiliations

Miyamura K
Department of Epidemiology, National Institute of Infectious Diseases, Tokyo, Japan.
Sato TA
No affiliation info available
Sakae K
No affiliation info available
Kato N
No affiliation info available
Ogino T
No affiliation info available
Yashima T
No affiliation info available
Sasagawa A
No affiliation info available
Chikahira M
No affiliation info available
Itagaki A
No affiliation info available
Katsuki K
No affiliation info available
Matsunaga Y
No affiliation info available
Utagawa E
No affiliation info available
Takeda N
No affiliation info available
Inouye S
No affiliation info available
Yamazaki S
No affiliation info available

MeSH

AdolescentAdultAge DistributionAgglutination TestsAnimalsAntibodies, ViralChildChild, PreschoolGelatinHemagglutination Inhibition TestsHumansInfantJapanMeaslesPrevalenceSensitivity and SpecificitySeroepidemiologic StudiesVaccination

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9413505

Citation

Miyamura, K, et al. "Comparison of Gelatin Particle Agglutination and Hemagglutination Inhibition Tests for Measles Seroepidemiology Studies." Archives of Virology, vol. 142, no. 10, 1997, pp. 1963-70.
Miyamura K, Sato TA, Sakae K, et al. Comparison of gelatin particle agglutination and hemagglutination inhibition tests for measles seroepidemiology studies. Arch Virol. 1997;142(10):1963-70.
Miyamura, K., Sato, T. A., Sakae, K., Kato, N., Ogino, T., Yashima, T., Sasagawa, A., Chikahira, M., Itagaki, A., Katsuki, K., Matsunaga, Y., Utagawa, E., Takeda, N., Inouye, S., & Yamazaki, S. (1997). Comparison of gelatin particle agglutination and hemagglutination inhibition tests for measles seroepidemiology studies. Archives of Virology, 142(10), 1963-70.
Miyamura K, et al. Comparison of Gelatin Particle Agglutination and Hemagglutination Inhibition Tests for Measles Seroepidemiology Studies. Arch Virol. 1997;142(10):1963-70. PubMed PMID: 9413505.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of gelatin particle agglutination and hemagglutination inhibition tests for measles seroepidemiology studies. AU - Miyamura,K, AU - Sato,T A, AU - Sakae,K, AU - Kato,N, AU - Ogino,T, AU - Yashima,T, AU - Sasagawa,A, AU - Chikahira,M, AU - Itagaki,A, AU - Katsuki,K, AU - Matsunaga,Y, AU - Utagawa,E, AU - Takeda,N, AU - Inouye,S, AU - Yamazaki,S, PY - 1997/1/1/pubmed PY - 1997/12/31/medline PY - 1997/1/1/entrez SP - 1963 EP - 70 JF - Archives of virology JO - Arch Virol VL - 142 IS - 10 N2 - The prevalence of measles antibody in Japan was surveyed with a newly developed gelatin particle agglutination (PA) test, and the results compared with those of the hemagglutination inhibition (HI) test. The two age-distribution curves of the PA antibody-positive rates at > or = 1:8 and > or = 1:32 were almost the same in all the age groups, except the less-than-1-year-old group for which the rate at > or = 1:8 was higher than that at > or = 1:32 (p < 0.05, chi 2 test). In the vaccinated children, all groups older-than-1-year of age had antibody-positive levels of 96% or more. In contrast, in the unvaccinated children, there was a sharp increase in antibody-positive rates between the 1- and 4-year-old groups, indicative that about 80% of the children were infected by wild measles virus at these ages. A significant number of PA antibody-positive specimens were antibody-negative (< 1:8) by HI. The percentage of specimens in this category, PA (+) but HI (-), was greatest in infants less than one year old, and least in young children, but it increased with age to 97% of the HI (-) specimens from adults of more than 20 years of age. The PA test therefore detected some measles antibodies that HI could not. This test is simple and useful for making serosurveys in both developed and developing countries. SN - 0304-8608 UR - https://www.unboundmedicine.com/medline/citation/9413505/Comparison_of_gelatin_particle_agglutination_and_hemagglutination_inhibition_tests_for_measles_seroepidemiology_studies_ L2 - https://dx.doi.org/10.1007/s007050050214 DB - PRIME DP - Unbound Medicine ER -