Tags

Type your tag names separated by a space and hit enter

Genotypic analysis of cutaneous T-cell lymphoma: a comparative study of Southern blot analysis with polymerase chain reaction amplification of the T-cell receptor-gamma gene.
Br J Dermatol. 1997 Nov; 137(5):673-9.BJ

Abstract

The diagnosis of early cutaneous T-cell lymphoma (CTCL) is a difficult point in dermatology. Recently, Southern blot analysis (SBA) and polymerase chain reaction (PCR) have been used to detect clonality in initial lesions in which clinical and histological findings are unspecific. Forty-one samples from 25 patients with CTCL were investigated for the presence of T-cell receptor-gamma gene rearrangement using a nested PCR technique and analysed by polyacrylamide gel electrophoresis (PAGE). Conventional SBA was also performed on 28 samples from 20 of these patients. In addition, 20 samples corresponding to patients with large plaque parapsoriasis (LPP), cutaneous B-cell lymphoma (CBCL) and eczema were analysed by PCR in the same way as were the CTCL specimens. Most of the CTCL specimens (81%) showed clonality on PCR analysis. Among patients with mycosis fungoides, 71% of initial patch lesions and 100% of plaques and tumours showed clonal disease. Clonality could be detected in three of four histologically negative post-treatment lesions. Clonal rearrangement was detected in one of three patients with LPP and in three of 10 patients with CBCL. None of the samples corresponding to patients with eczema showed positive results. SBA was significantly less sensitive than PCR in detecting clonality in CTCL patients (42% among early disease and 60% among advanced cases). The results indicate that this PCR/PAGE technique is a reliable and useful method for the detection of clonality in early skin lesions of CTCL patients and probably in the identification of silent extracutaneous involvement.

Authors+Show Affiliations

Department of Dermatology, Hospital Princeps d'Espanya, Ciutat Sanitària i Universitària de Bellvitge, Barcelona, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9415223

Citation

Curcó, N, et al. "Genotypic Analysis of Cutaneous T-cell Lymphoma: a Comparative Study of Southern Blot Analysis With Polymerase Chain Reaction Amplification of the T-cell Receptor-gamma Gene." The British Journal of Dermatology, vol. 137, no. 5, 1997, pp. 673-9.
Curcó N, Servitje O, Llucià M, et al. Genotypic analysis of cutaneous T-cell lymphoma: a comparative study of Southern blot analysis with polymerase chain reaction amplification of the T-cell receptor-gamma gene. Br J Dermatol. 1997;137(5):673-9.
Curcó, N., Servitje, O., Llucià, M., Bertran, J., Limón, A., Carmona, M., Romagosa, V., & Peyrí, J. (1997). Genotypic analysis of cutaneous T-cell lymphoma: a comparative study of Southern blot analysis with polymerase chain reaction amplification of the T-cell receptor-gamma gene. The British Journal of Dermatology, 137(5), 673-9.
Curcó N, et al. Genotypic Analysis of Cutaneous T-cell Lymphoma: a Comparative Study of Southern Blot Analysis With Polymerase Chain Reaction Amplification of the T-cell Receptor-gamma Gene. Br J Dermatol. 1997;137(5):673-9. PubMed PMID: 9415223.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotypic analysis of cutaneous T-cell lymphoma: a comparative study of Southern blot analysis with polymerase chain reaction amplification of the T-cell receptor-gamma gene. AU - Curcó,N, AU - Servitje,O, AU - Llucià,M, AU - Bertran,J, AU - Limón,A, AU - Carmona,M, AU - Romagosa,V, AU - Peyrí,J, PY - 1998/2/12/pubmed PY - 2000/6/10/medline PY - 1998/2/12/entrez SP - 673 EP - 9 JF - The British journal of dermatology JO - Br J Dermatol VL - 137 IS - 5 N2 - The diagnosis of early cutaneous T-cell lymphoma (CTCL) is a difficult point in dermatology. Recently, Southern blot analysis (SBA) and polymerase chain reaction (PCR) have been used to detect clonality in initial lesions in which clinical and histological findings are unspecific. Forty-one samples from 25 patients with CTCL were investigated for the presence of T-cell receptor-gamma gene rearrangement using a nested PCR technique and analysed by polyacrylamide gel electrophoresis (PAGE). Conventional SBA was also performed on 28 samples from 20 of these patients. In addition, 20 samples corresponding to patients with large plaque parapsoriasis (LPP), cutaneous B-cell lymphoma (CBCL) and eczema were analysed by PCR in the same way as were the CTCL specimens. Most of the CTCL specimens (81%) showed clonality on PCR analysis. Among patients with mycosis fungoides, 71% of initial patch lesions and 100% of plaques and tumours showed clonal disease. Clonality could be detected in three of four histologically negative post-treatment lesions. Clonal rearrangement was detected in one of three patients with LPP and in three of 10 patients with CBCL. None of the samples corresponding to patients with eczema showed positive results. SBA was significantly less sensitive than PCR in detecting clonality in CTCL patients (42% among early disease and 60% among advanced cases). The results indicate that this PCR/PAGE technique is a reliable and useful method for the detection of clonality in early skin lesions of CTCL patients and probably in the identification of silent extracutaneous involvement. SN - 0007-0963 UR - https://www.unboundmedicine.com/medline/citation/9415223/Genotypic_analysis_of_cutaneous_T_cell_lymphoma:_a_comparative_study_of_Southern_blot_analysis_with_polymerase_chain_reaction_amplification_of_the_T_cell_receptor_gamma_gene_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0007-0963&date=1997&volume=137&issue=5&spage=673 DB - PRIME DP - Unbound Medicine ER -