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Variability in cytokine and cell adhesion molecule staining in arthroscopic synovial biopsies: quantification using color video image analysis.
J Rheumatol. 1997 Dec; 24(12):2291-8.JR

Abstract

OBJECTIVE

To investigate the variability in immunostaining for cytokines and cell adhesion molecules using multiple arthroscopically directed synovial biopsies from within a rheumatoid knee joint, quantitated by color video image analysis.

METHODS

Needle arthroscopic biopsies were taken from multiple sites (4-7 sites) around a knee joint in 8 patients with rheumatoid arthritis (RA). In 5 patients, immunoperoxidase staining for the cytokines tumor necrosis factor alpha (TNF-alpha), interleukin 8 (IL-8), and IL-1beta as well as the IL-1 receptor antagonist protein (IL-1ra) was performed. In 3 patients, immunoperoxidase staining for the cell adhesion molecules E-selectin (CD62E), P-selectin (CD62P), intercellular adhesion molecule 1 (ICAM-1, CD54), and platelet endothelial cell adhesion molecule (PECAM, CD31) was performed. Immunostaining was quantified using color video image analysis.

RESULTS

The overall probability of paired biopsies from the same RA knee joint being significantly different from each other due to sampling variation was at most 22% for cytokine staining (usually less than 10%). There were no significant differences between intrabiopsy and interbiopsy variability for cell adhesion molecule staining of the sublining and vessels.

CONCLUSION

The variability in cytokine and cell adhesion molecule staining within any single biopsy usually reflects the variability between biopsies taken from different sites in the same rheumatoid joint when the immunostaining is quantified using color video image analysis. Therefore, only a small number of synovial biopsies are required to accurately determine the cytokine and cell adhesion molecule expression in a single joint.

Authors+Show Affiliations

Department of Pathology, Repatriation General Hospital, Daw Park, SA, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9415630

Citation

Youssef, P P., et al. "Variability in Cytokine and Cell Adhesion Molecule Staining in Arthroscopic Synovial Biopsies: Quantification Using Color Video Image Analysis." The Journal of Rheumatology, vol. 24, no. 12, 1997, pp. 2291-8.
Youssef PP, Triantafillou S, Parker A, et al. Variability in cytokine and cell adhesion molecule staining in arthroscopic synovial biopsies: quantification using color video image analysis. J Rheumatol. 1997;24(12):2291-8.
Youssef, P. P., Triantafillou, S., Parker, A., Coleman, M., Roberts-Thomson, P. J., Ahern, M. J., & Smith, M. D. (1997). Variability in cytokine and cell adhesion molecule staining in arthroscopic synovial biopsies: quantification using color video image analysis. The Journal of Rheumatology, 24(12), 2291-8.
Youssef PP, et al. Variability in Cytokine and Cell Adhesion Molecule Staining in Arthroscopic Synovial Biopsies: Quantification Using Color Video Image Analysis. J Rheumatol. 1997;24(12):2291-8. PubMed PMID: 9415630.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Variability in cytokine and cell adhesion molecule staining in arthroscopic synovial biopsies: quantification using color video image analysis. AU - Youssef,P P, AU - Triantafillou,S, AU - Parker,A, AU - Coleman,M, AU - Roberts-Thomson,P J, AU - Ahern,M J, AU - Smith,M D, PY - 1998/2/7/pubmed PY - 1998/2/7/medline PY - 1998/2/7/entrez SP - 2291 EP - 8 JF - The Journal of rheumatology JO - J Rheumatol VL - 24 IS - 12 N2 - OBJECTIVE: To investigate the variability in immunostaining for cytokines and cell adhesion molecules using multiple arthroscopically directed synovial biopsies from within a rheumatoid knee joint, quantitated by color video image analysis. METHODS: Needle arthroscopic biopsies were taken from multiple sites (4-7 sites) around a knee joint in 8 patients with rheumatoid arthritis (RA). In 5 patients, immunoperoxidase staining for the cytokines tumor necrosis factor alpha (TNF-alpha), interleukin 8 (IL-8), and IL-1beta as well as the IL-1 receptor antagonist protein (IL-1ra) was performed. In 3 patients, immunoperoxidase staining for the cell adhesion molecules E-selectin (CD62E), P-selectin (CD62P), intercellular adhesion molecule 1 (ICAM-1, CD54), and platelet endothelial cell adhesion molecule (PECAM, CD31) was performed. Immunostaining was quantified using color video image analysis. RESULTS: The overall probability of paired biopsies from the same RA knee joint being significantly different from each other due to sampling variation was at most 22% for cytokine staining (usually less than 10%). There were no significant differences between intrabiopsy and interbiopsy variability for cell adhesion molecule staining of the sublining and vessels. CONCLUSION: The variability in cytokine and cell adhesion molecule staining within any single biopsy usually reflects the variability between biopsies taken from different sites in the same rheumatoid joint when the immunostaining is quantified using color video image analysis. Therefore, only a small number of synovial biopsies are required to accurately determine the cytokine and cell adhesion molecule expression in a single joint. SN - 0315-162X UR - https://www.unboundmedicine.com/medline/citation/9415630/Variability_in_cytokine_and_cell_adhesion_molecule_staining_in_arthroscopic_synovial_biopsies:_quantification_using_color_video_image_analysis_ L2 - https://antibodies.cancer.gov/detail/CPTC-IL1RN-1 DB - PRIME DP - Unbound Medicine ER -