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Epstein-Barr virus infection in non-carcinomatous gastric epithelium.
J Pathol. 1997 Nov; 183(3):293-8.JP

Abstract

Gastric tissue specimens from 20 patients with chronic atrophic gastritis, one of whom also had an early gastric carcinoma, were studied for evidence of Epstein-Barr virus (EBV) infection by Southern blot analysis, DNA and RNA in situ hybridization, and immunohistochemistry for the presence of the EBV-determined nuclear antigen 1 (EBNA-1) and the latent membrane protein 1 (LMP-1). EBV DNA was detected in two cases with chronic atrophic gastritis and in the case with early gastric carcinoma by Southern blot hybridization. DNA in situ hybridization showed EBV genomes in the epithelial cells of two other cases with chronic atrophic gastritis and in non-carcinomatous and carcinomatous epithelial cells of the early gastric carcinoma case. EBNA-1 was detected in all cases. LMP-1 was detected in areas of intestinal metaplasia in eight patients with chronic atrophic gastritis. EBV-encoded small RNA 1 (EBER-1) expression was limited to carcinoma cells. These results show that gastric epithelium is frequently infected with EBV and suggest that prolonged EBV persistence may contribute to the development of gastric carcinoma.

Authors+Show Affiliations

First Department of Internal Medicine, Yamaguchi University School of Medicine, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9422984

Citation

Yanai, H, et al. "Epstein-Barr Virus Infection in Non-carcinomatous Gastric Epithelium." The Journal of Pathology, vol. 183, no. 3, 1997, pp. 293-8.
Yanai H, Takada K, Shimizu N, et al. Epstein-Barr virus infection in non-carcinomatous gastric epithelium. J Pathol. 1997;183(3):293-8.
Yanai, H., Takada, K., Shimizu, N., Mizugaki, Y., Tada, M., & Okita, K. (1997). Epstein-Barr virus infection in non-carcinomatous gastric epithelium. The Journal of Pathology, 183(3), 293-8.
Yanai H, et al. Epstein-Barr Virus Infection in Non-carcinomatous Gastric Epithelium. J Pathol. 1997;183(3):293-8. PubMed PMID: 9422984.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epstein-Barr virus infection in non-carcinomatous gastric epithelium. AU - Yanai,H, AU - Takada,K, AU - Shimizu,N, AU - Mizugaki,Y, AU - Tada,M, AU - Okita,K, PY - 1998/1/10/pubmed PY - 2000/6/20/medline PY - 1998/1/10/entrez SP - 293 EP - 8 JF - The Journal of pathology JO - J. Pathol. VL - 183 IS - 3 N2 - Gastric tissue specimens from 20 patients with chronic atrophic gastritis, one of whom also had an early gastric carcinoma, were studied for evidence of Epstein-Barr virus (EBV) infection by Southern blot analysis, DNA and RNA in situ hybridization, and immunohistochemistry for the presence of the EBV-determined nuclear antigen 1 (EBNA-1) and the latent membrane protein 1 (LMP-1). EBV DNA was detected in two cases with chronic atrophic gastritis and in the case with early gastric carcinoma by Southern blot hybridization. DNA in situ hybridization showed EBV genomes in the epithelial cells of two other cases with chronic atrophic gastritis and in non-carcinomatous and carcinomatous epithelial cells of the early gastric carcinoma case. EBNA-1 was detected in all cases. LMP-1 was detected in areas of intestinal metaplasia in eight patients with chronic atrophic gastritis. EBV-encoded small RNA 1 (EBER-1) expression was limited to carcinoma cells. These results show that gastric epithelium is frequently infected with EBV and suggest that prolonged EBV persistence may contribute to the development of gastric carcinoma. SN - 0022-3417 UR - https://www.unboundmedicine.com/medline/citation/9422984/Epstein_Barr_virus_infection_in_non_carcinomatous_gastric_epithelium_ L2 - https://doi.org/10.1002/(SICI)1096-9896(199711)183:3<293::AID-PATH937>3.0.CO;2-C DB - PRIME DP - Unbound Medicine ER -