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Lactation status influences expression of CCAAT/enhancer binding protein isoform mRNA in the mouse mammary gland.
J Cell Physiol. 1998 Feb; 174(2):232-9.JC

Abstract

The CCAAT/enhancer binding proteins (C/EBPs) are a highly conserved family of DNA binding proteins implicated in the transcriptional control of genes involved in cell growth and differentiation in a variety of tissues. The expression of C/EBP-alpha, beta, and delta mRNA in the normal mouse mammary gland was investigated during pregnancy, lactation, and involution via Northern blotting and in situ hybridization. Mammary gland C/EBP-alpha mRNA is detectable at low levels during pregnancy and postlactational involution. C/EBP-beta mRNA levels are elevated during pregnancy, decline slightly in midlactation, and are induced within 48 hours of the onset of involution. C/EBP-delta mRNA content is low throughout pregnancy and lactation, but increases dramatically (>100-fold) within 12 hours after the onset of postlactational involution. In situ hybridization demonstrates that mammary epithelial cells are responsible for the expression of C/EBP-delta mRNA during involution. In contrast to mammary gland, C/EBP-alpha is the predominate isoform expressed in liver with relatively low expression of C/EBP-beta and C/EBP-delta mRNA. Liver C/EBP isoform mRNA levels are unaffected by lactation status. These results demonstrate the tissue-specific regulation of C/EBPs. The pronounced sequential induction of C/EBP-delta and C/EBP-beta during postlactational involution is consistent with a role for C/EBPs in the regulation of mammary epithelial cell apoptosis.

Authors+Show Affiliations

Department of Veterinary Biosciences, The Ohio State University, Columbus 43210, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9428809

Citation

Gigliotti, A P., and J W. DeWille. "Lactation Status Influences Expression of CCAAT/enhancer Binding Protein Isoform mRNA in the Mouse Mammary Gland." Journal of Cellular Physiology, vol. 174, no. 2, 1998, pp. 232-9.
Gigliotti AP, DeWille JW. Lactation status influences expression of CCAAT/enhancer binding protein isoform mRNA in the mouse mammary gland. J Cell Physiol. 1998;174(2):232-9.
Gigliotti, A. P., & DeWille, J. W. (1998). Lactation status influences expression of CCAAT/enhancer binding protein isoform mRNA in the mouse mammary gland. Journal of Cellular Physiology, 174(2), 232-9.
Gigliotti AP, DeWille JW. Lactation Status Influences Expression of CCAAT/enhancer Binding Protein Isoform mRNA in the Mouse Mammary Gland. J Cell Physiol. 1998;174(2):232-9. PubMed PMID: 9428809.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lactation status influences expression of CCAAT/enhancer binding protein isoform mRNA in the mouse mammary gland. AU - Gigliotti,A P, AU - DeWille,J W, PY - 1998/1/15/pubmed PY - 2000/6/20/medline PY - 1998/1/15/entrez SP - 232 EP - 9 JF - Journal of cellular physiology JO - J Cell Physiol VL - 174 IS - 2 N2 - The CCAAT/enhancer binding proteins (C/EBPs) are a highly conserved family of DNA binding proteins implicated in the transcriptional control of genes involved in cell growth and differentiation in a variety of tissues. The expression of C/EBP-alpha, beta, and delta mRNA in the normal mouse mammary gland was investigated during pregnancy, lactation, and involution via Northern blotting and in situ hybridization. Mammary gland C/EBP-alpha mRNA is detectable at low levels during pregnancy and postlactational involution. C/EBP-beta mRNA levels are elevated during pregnancy, decline slightly in midlactation, and are induced within 48 hours of the onset of involution. C/EBP-delta mRNA content is low throughout pregnancy and lactation, but increases dramatically (>100-fold) within 12 hours after the onset of postlactational involution. In situ hybridization demonstrates that mammary epithelial cells are responsible for the expression of C/EBP-delta mRNA during involution. In contrast to mammary gland, C/EBP-alpha is the predominate isoform expressed in liver with relatively low expression of C/EBP-beta and C/EBP-delta mRNA. Liver C/EBP isoform mRNA levels are unaffected by lactation status. These results demonstrate the tissue-specific regulation of C/EBPs. The pronounced sequential induction of C/EBP-delta and C/EBP-beta during postlactational involution is consistent with a role for C/EBPs in the regulation of mammary epithelial cell apoptosis. SN - 0021-9541 UR - https://www.unboundmedicine.com/medline/citation/9428809/Lactation_status_influences_expression_of_CCAAT/enhancer_binding_protein_isoform_mRNA_in_the_mouse_mammary_gland_ L2 - https://doi.org/10.1002/(SICI)1097-4652(199802)174:2<232::AID-JCP10>3.0.CO;2-E DB - PRIME DP - Unbound Medicine ER -