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The lipolysis of chylomicrons derived from different dietary fats by lipoprotein lipase in vitro.
Biochim Biophys Acta. 1997 Nov 30; 1349(3):257-63.BB

Abstract

The lipolysis of chylomicrons derived from palm, olive, corn or fish oil (enriched in saturated, monounsaturated, n - 6 polyunsaturated and n - 3 polyunsaturated fatty acids, respectively) by rat post-heparin lipoprotein lipase in vitro was compared by measuring the release of [3H]oleate from their triacylglycerol. Chylomicrons derived from corn oil were lipolysed more rapidly than the other types in the first 20 min of the reaction, but after 120 min the total amount of triacylglycerol hydrolysed was similar with all types of chylomicrons used. The rate of lipolysis of the different types of chylomicrons also showed different dependencies on the substrate concentration. The highest Vmax values were obtained when the chylomicrons were derived from olive and corn oil and the lowest when they were derived from palm oil, while olive oil chylomicrons gave the highest Km and palm oil chylomicrons the lowest. These results indicate that differential metabolism of chylomicrons of different fatty acid composition by lipoprotein lipase may play a part in the differential rates of clearance from the blood of lipid of dietary origin demonstrated in earlier work from our laboratory.

Authors+Show Affiliations

Department of Veterinary Basic Sciences, The Royal Veterinary College, London, UK.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9434140

Citation

Botham, K M., et al. "The Lipolysis of Chylomicrons Derived From Different Dietary Fats By Lipoprotein Lipase in Vitro." Biochimica Et Biophysica Acta, vol. 1349, no. 3, 1997, pp. 257-63.
Botham KM, Avella M, Cantafora A, et al. The lipolysis of chylomicrons derived from different dietary fats by lipoprotein lipase in vitro. Biochim Biophys Acta. 1997;1349(3):257-63.
Botham, K. M., Avella, M., Cantafora, A., & Bravo, E. (1997). The lipolysis of chylomicrons derived from different dietary fats by lipoprotein lipase in vitro. Biochimica Et Biophysica Acta, 1349(3), 257-63.
Botham KM, et al. The Lipolysis of Chylomicrons Derived From Different Dietary Fats By Lipoprotein Lipase in Vitro. Biochim Biophys Acta. 1997 Nov 30;1349(3):257-63. PubMed PMID: 9434140.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The lipolysis of chylomicrons derived from different dietary fats by lipoprotein lipase in vitro. AU - Botham,K M, AU - Avella,M, AU - Cantafora,A, AU - Bravo,E, PY - 1998/1/20/pubmed PY - 1998/1/20/medline PY - 1998/1/20/entrez SP - 257 EP - 63 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 1349 IS - 3 N2 - The lipolysis of chylomicrons derived from palm, olive, corn or fish oil (enriched in saturated, monounsaturated, n - 6 polyunsaturated and n - 3 polyunsaturated fatty acids, respectively) by rat post-heparin lipoprotein lipase in vitro was compared by measuring the release of [3H]oleate from their triacylglycerol. Chylomicrons derived from corn oil were lipolysed more rapidly than the other types in the first 20 min of the reaction, but after 120 min the total amount of triacylglycerol hydrolysed was similar with all types of chylomicrons used. The rate of lipolysis of the different types of chylomicrons also showed different dependencies on the substrate concentration. The highest Vmax values were obtained when the chylomicrons were derived from olive and corn oil and the lowest when they were derived from palm oil, while olive oil chylomicrons gave the highest Km and palm oil chylomicrons the lowest. These results indicate that differential metabolism of chylomicrons of different fatty acid composition by lipoprotein lipase may play a part in the differential rates of clearance from the blood of lipid of dietary origin demonstrated in earlier work from our laboratory. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/9434140/The_lipolysis_of_chylomicrons_derived_from_different_dietary_fats_by_lipoprotein_lipase_in_vitro_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0005-2760(97)00134-3 DB - PRIME DP - Unbound Medicine ER -