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Characterization of Erwinia carotovora subsp. carotovora LY34 endo-1,4-beta-glucanase genes and rapid identification of their gene products.
Biochem Biophys Res Commun. 1997 Dec 29; 241(3):636-41.BB

Abstract

Genomic DNA of the phytopathogenic Erwinia carotovora subsp. carotovora LY34 was partially digested with Sau3AI, ligated into the BamHI site of pBlue-script II SK+, and introduced into E. coli. Two clones that were able to hydrolyse carboxymethylcellulose were selected. 1.5 kb and 1.2 kb fragments containing the celA and celB genes, respectively, were subcloned and sequenced. The celA and celB genes had open reading frames of 1,161 bp and 792 bp encoding 487 and 264 amino acid residues with calculated molecular weights of 42,003 Da and 29,890 Da, respectively. Each, CelA and CelB, carried a typical prokaryotic signal peptide of 32 and 36 amino acid residues, respectively. The apparent molecular masses of the proteins when expressed in E. coli cells were approximately 39 kDa (CelA) and 26 kDa (CelB) as assessed by CMC-SDS-PAGE. Activity staining of CMCase in an SDS-PAGE gel containing 0.1% CMC revealed that the cloned endoglucanase isozymes comigrated with the corresponding ones present in Erwinia carotovora subsp. carotovora LY34.

Authors+Show Affiliations

Department of Agricultural Chemistry, Gyeongsang National University, Chinju, Korea.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9434760

Citation

Park, Y W., et al. "Characterization of Erwinia Carotovora Subsp. Carotovora LY34 Endo-1,4-beta-glucanase Genes and Rapid Identification of Their Gene Products." Biochemical and Biophysical Research Communications, vol. 241, no. 3, 1997, pp. 636-41.
Park YW, Lim ST, Cho SJ, et al. Characterization of Erwinia carotovora subsp. carotovora LY34 endo-1,4-beta-glucanase genes and rapid identification of their gene products. Biochem Biophys Res Commun. 1997;241(3):636-41.
Park, Y. W., Lim, S. T., Cho, S. J., & Yun, H. D. (1997). Characterization of Erwinia carotovora subsp. carotovora LY34 endo-1,4-beta-glucanase genes and rapid identification of their gene products. Biochemical and Biophysical Research Communications, 241(3), 636-41.
Park YW, et al. Characterization of Erwinia Carotovora Subsp. Carotovora LY34 Endo-1,4-beta-glucanase Genes and Rapid Identification of Their Gene Products. Biochem Biophys Res Commun. 1997 Dec 29;241(3):636-41. PubMed PMID: 9434760.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of Erwinia carotovora subsp. carotovora LY34 endo-1,4-beta-glucanase genes and rapid identification of their gene products. AU - Park,Y W, AU - Lim,S T, AU - Cho,S J, AU - Yun,H D, PY - 1998/1/22/pubmed PY - 1998/1/22/medline PY - 1998/1/22/entrez SP - 636 EP - 41 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 241 IS - 3 N2 - Genomic DNA of the phytopathogenic Erwinia carotovora subsp. carotovora LY34 was partially digested with Sau3AI, ligated into the BamHI site of pBlue-script II SK+, and introduced into E. coli. Two clones that were able to hydrolyse carboxymethylcellulose were selected. 1.5 kb and 1.2 kb fragments containing the celA and celB genes, respectively, were subcloned and sequenced. The celA and celB genes had open reading frames of 1,161 bp and 792 bp encoding 487 and 264 amino acid residues with calculated molecular weights of 42,003 Da and 29,890 Da, respectively. Each, CelA and CelB, carried a typical prokaryotic signal peptide of 32 and 36 amino acid residues, respectively. The apparent molecular masses of the proteins when expressed in E. coli cells were approximately 39 kDa (CelA) and 26 kDa (CelB) as assessed by CMC-SDS-PAGE. Activity staining of CMCase in an SDS-PAGE gel containing 0.1% CMC revealed that the cloned endoglucanase isozymes comigrated with the corresponding ones present in Erwinia carotovora subsp. carotovora LY34. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/9434760/Characterization_of_Erwinia_carotovora_subsp__carotovora_LY34_endo_14_beta_glucanase_genes_and_rapid_identification_of_their_gene_products_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(97)97747-0 DB - PRIME DP - Unbound Medicine ER -