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Determination of morphine and its 3- and 6-glucuronides, codeine, codeine-glucuronide and 6-monoacetylmorphine in body fluids by liquid chromatography atmospheric pressure chemical ionization mass spectrometry.
J Chromatogr B Biomed Sci Appl. 1997 Dec 05; 703(1-2):115-27.JC

Abstract

A selective assay of morphine-3-glucuronide (M3G), morphine-6-glucuronide (M6G), morphine, codeine, codeine-6-glucuronide (C6G) and 6-monoacetylmorphine (6-MAM) based on liquid chromatography atmospheric pressure chemical ionization mass spectrometry (LC-APCI-MS) is described. The drugs were extracted from serum, autopsy blood, urine, cerebrospinal fluid or vitreous humor using C18 solid-phase extraction cartridges and subjected to LC-APCI-MS analysis. The separation was performed on an ODS column in acetonitrile-50 mM ammonium formate buffer, pH 3.0 (5:95), using a flow-rate gradient from 0.6 to 1.1 ml/min (total analysis time was 17 min). The quantitative analysis was done using deuterated analogues of each compound. Selected-ion monitoring detection was applied: m/z 286 (for morphine, M3G-aglycone and M6G-aglycone), 289 (for morphine-d3, M3G-d3-aglycone and M6G-d3-aglycone), 300 (for codeine and C6G-aglycone), 303 (for C6G-d3-aglycone), 306 (for codeine-d6), 328 (for 6-MAM), 334 (for 6-MAM-d6), 462 (for M3G and M6G), 465 (for M3G-d3 and M6G-d3), 476 (for C6G) and 479 (for C6G-d3). The limits of quantitation were: 1 microg/l for morphine, 2 microg/l for 6-MAM, 5 microg/l for M3G, M6G and codeine and 200 microg/I for C6G. The recovery ranged from 85 to 98% for each analyte. The method appeared very selective and may be used for the routine determination of opiates in body fluids of heroin abusers and patients treated with opiates.

Authors+Show Affiliations

Bogusz MJ
Institute of Forensic Medicine, Aachen University of Technology, Germany.
Maier RD
No affiliation info available
Erkens M
No affiliation info available
Driessen S
No affiliation info available

MeSH

Analgesics, OpioidBody FluidsChromatography, LiquidCodeineHumansMass SpectrometryMorphineMorphine DerivativesReproducibility of ResultsSensitivity and Specificity

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

9448068

Citation

Bogusz, M J., et al. "Determination of Morphine and Its 3- and 6-glucuronides, Codeine, Codeine-glucuronide and 6-monoacetylmorphine in Body Fluids By Liquid Chromatography Atmospheric Pressure Chemical Ionization Mass Spectrometry." Journal of Chromatography. B, Biomedical Sciences and Applications, vol. 703, no. 1-2, 1997, pp. 115-27.
Bogusz MJ, Maier RD, Erkens M, et al. Determination of morphine and its 3- and 6-glucuronides, codeine, codeine-glucuronide and 6-monoacetylmorphine in body fluids by liquid chromatography atmospheric pressure chemical ionization mass spectrometry. J Chromatogr B Biomed Sci Appl. 1997;703(1-2):115-27.
Bogusz, M. J., Maier, R. D., Erkens, M., & Driessen, S. (1997). Determination of morphine and its 3- and 6-glucuronides, codeine, codeine-glucuronide and 6-monoacetylmorphine in body fluids by liquid chromatography atmospheric pressure chemical ionization mass spectrometry. Journal of Chromatography. B, Biomedical Sciences and Applications, 703(1-2), 115-27.
Bogusz MJ, et al. Determination of Morphine and Its 3- and 6-glucuronides, Codeine, Codeine-glucuronide and 6-monoacetylmorphine in Body Fluids By Liquid Chromatography Atmospheric Pressure Chemical Ionization Mass Spectrometry. J Chromatogr B Biomed Sci Appl. 1997 Dec 5;703(1-2):115-27. PubMed PMID: 9448068.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of morphine and its 3- and 6-glucuronides, codeine, codeine-glucuronide and 6-monoacetylmorphine in body fluids by liquid chromatography atmospheric pressure chemical ionization mass spectrometry. AU - Bogusz,M J, AU - Maier,R D, AU - Erkens,M, AU - Driessen,S, PY - 1998/2/3/pubmed PY - 1998/2/3/medline PY - 1998/2/3/entrez SP - 115 EP - 27 JF - Journal of chromatography. B, Biomedical sciences and applications JO - J Chromatogr B Biomed Sci Appl VL - 703 IS - 1-2 N2 - A selective assay of morphine-3-glucuronide (M3G), morphine-6-glucuronide (M6G), morphine, codeine, codeine-6-glucuronide (C6G) and 6-monoacetylmorphine (6-MAM) based on liquid chromatography atmospheric pressure chemical ionization mass spectrometry (LC-APCI-MS) is described. The drugs were extracted from serum, autopsy blood, urine, cerebrospinal fluid or vitreous humor using C18 solid-phase extraction cartridges and subjected to LC-APCI-MS analysis. The separation was performed on an ODS column in acetonitrile-50 mM ammonium formate buffer, pH 3.0 (5:95), using a flow-rate gradient from 0.6 to 1.1 ml/min (total analysis time was 17 min). The quantitative analysis was done using deuterated analogues of each compound. Selected-ion monitoring detection was applied: m/z 286 (for morphine, M3G-aglycone and M6G-aglycone), 289 (for morphine-d3, M3G-d3-aglycone and M6G-d3-aglycone), 300 (for codeine and C6G-aglycone), 303 (for C6G-d3-aglycone), 306 (for codeine-d6), 328 (for 6-MAM), 334 (for 6-MAM-d6), 462 (for M3G and M6G), 465 (for M3G-d3 and M6G-d3), 476 (for C6G) and 479 (for C6G-d3). The limits of quantitation were: 1 microg/l for morphine, 2 microg/l for 6-MAM, 5 microg/l for M3G, M6G and codeine and 200 microg/I for C6G. The recovery ranged from 85 to 98% for each analyte. The method appeared very selective and may be used for the routine determination of opiates in body fluids of heroin abusers and patients treated with opiates. SN - 1387-2273 UR - https://www.unboundmedicine.com/medline/citation/9448068/Determination_of_morphine_and_its_3__and_6_glucuronides_codeine_codeine_glucuronide_and_6_monoacetylmorphine_in_body_fluids_by_liquid_chromatography_atmospheric_pressure_chemical_ionization_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -
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