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Sequence and structural elements of methylation guide snoRNAs essential for site-specific ribose methylation of pre-rRNA.
EMBO J. 1998 Feb 02; 17(3):797-807.EJ

Abstract

Site-specific 2'-O-ribose methylation of eukaryotic rRNAs is guided by small nucleolar RNAs (snoRNAs). The methylation guide snoRNAs carry long perfect complementaries to rRNAs. These antisense elements are located either in the 5' half or in the 3' end region of the snoRNA, and are followed by the conserved D' or D box motifs, respectively. An uninterrupted helix formed between the rRNA and the antisense element of the snoRNA, in conjunction with the adjacent D' or D box, constitute the recognition signal for the putative methyltransferase. Here, we have identified an additional essential box element common to methylation guide snoRNAs, termed the C' box. We show that the C' box functions in concert with the D' box and plays a crucial role in the methyltransfer reaction directed by the upstream antisense element and the D' box. We also show that an internal fragment of U24 methylation guide snoRNA, encompassing the upstream antisense element and the D' and C' box motifs, can support the site-specific methylation of rRNA. This strongly suggests that the C box of methylation guide snoRNAs plays an essential role in the methyltransfer reaction guided by the 3'-terminal antisense element and the D box of the snoRNA.

Authors+Show Affiliations

Laboratoire de Biologie Moléculaire Eucaryote du Centre National de la Recherche Scientifique, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex, France.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9451004

Citation

Kiss-László, Z, et al. "Sequence and Structural Elements of Methylation Guide snoRNAs Essential for Site-specific Ribose Methylation of Pre-rRNA." The EMBO Journal, vol. 17, no. 3, 1998, pp. 797-807.
Kiss-László Z, Henry Y, Kiss T. Sequence and structural elements of methylation guide snoRNAs essential for site-specific ribose methylation of pre-rRNA. EMBO J. 1998;17(3):797-807.
Kiss-László, Z., Henry, Y., & Kiss, T. (1998). Sequence and structural elements of methylation guide snoRNAs essential for site-specific ribose methylation of pre-rRNA. The EMBO Journal, 17(3), 797-807.
Kiss-László Z, Henry Y, Kiss T. Sequence and Structural Elements of Methylation Guide snoRNAs Essential for Site-specific Ribose Methylation of Pre-rRNA. EMBO J. 1998 Feb 2;17(3):797-807. PubMed PMID: 9451004.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sequence and structural elements of methylation guide snoRNAs essential for site-specific ribose methylation of pre-rRNA. AU - Kiss-László,Z, AU - Henry,Y, AU - Kiss,T, PY - 1998/3/14/pubmed PY - 1998/3/14/medline PY - 1998/3/14/entrez SP - 797 EP - 807 JF - The EMBO journal JO - EMBO J VL - 17 IS - 3 N2 - Site-specific 2'-O-ribose methylation of eukaryotic rRNAs is guided by small nucleolar RNAs (snoRNAs). The methylation guide snoRNAs carry long perfect complementaries to rRNAs. These antisense elements are located either in the 5' half or in the 3' end region of the snoRNA, and are followed by the conserved D' or D box motifs, respectively. An uninterrupted helix formed between the rRNA and the antisense element of the snoRNA, in conjunction with the adjacent D' or D box, constitute the recognition signal for the putative methyltransferase. Here, we have identified an additional essential box element common to methylation guide snoRNAs, termed the C' box. We show that the C' box functions in concert with the D' box and plays a crucial role in the methyltransfer reaction directed by the upstream antisense element and the D' box. We also show that an internal fragment of U24 methylation guide snoRNA, encompassing the upstream antisense element and the D' and C' box motifs, can support the site-specific methylation of rRNA. This strongly suggests that the C box of methylation guide snoRNAs plays an essential role in the methyltransfer reaction guided by the 3'-terminal antisense element and the D box of the snoRNA. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/9451004/Sequence_and_structural_elements_of_methylation_guide_snoRNAs_essential_for_site_specific_ribose_methylation_of_pre_rRNA_ L2 - https://doi.org/10.1093/emboj/17.3.797 DB - PRIME DP - Unbound Medicine ER -