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Curli fibers are highly conserved between Salmonella typhimurium and Escherichia coli with respect to operon structure and regulation.
J Bacteriol. 1998 Feb; 180(3):722-31.JB

Abstract

Mouse-virulent Salmonella typhimurium strains SR-11 and ATCC 14028-1s express curli fibers, thin aggregative fibers, at ambient temperature on plates as judged by Western blot analysis and electron microscopy. Concomitantly with curli expression, cells develop a rough and dry colony morphology and bind the dye Congo red (called the rdar morphotype). Cloning and characterization of the two divergently transcribed operons required for curli biogenesis, csgBA(C) and csgDEFG, from S. typhimurium SR-11 revealed the same gene order and flanking genes as in Escherichia coli. The divergence of the curli region between S. typhimurium and E. coli at the nucleotide level is above average (22.4%). However, a high level of conservation at the protein level, which ranged from 86% amino acid homology for the fiber subunit CsgA to 99% homology for the lipoprotein CsgG, implies functional constraints on the gene products. Consequently, S. typhimurium genes on low-copy-number plasmids were able to complement respective E. coli mutants, although not always to wild-type levels. rpoS and ompR are required for transcriptional activation of (at least) the csgD promoter. The high degree of conservation at the protein level and the identical regulation patterns in E. coli and S. typhimurium suggest similar roles of curli fibers in the same ecological niche in the two species.

Authors+Show Affiliations

Department of Bacteriology, Microbiology and Tumorbiology Center, Karolinska Institutet, Stockholm, Sweden. ute.roemling@mtc.ki.seNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9457880

Citation

Römling, U, et al. "Curli Fibers Are Highly Conserved Between Salmonella Typhimurium and Escherichia Coli With Respect to Operon Structure and Regulation." Journal of Bacteriology, vol. 180, no. 3, 1998, pp. 722-31.
Römling U, Bian Z, Hammar M, et al. Curli fibers are highly conserved between Salmonella typhimurium and Escherichia coli with respect to operon structure and regulation. J Bacteriol. 1998;180(3):722-31.
Römling, U., Bian, Z., Hammar, M., Sierralta, W. D., & Normark, S. (1998). Curli fibers are highly conserved between Salmonella typhimurium and Escherichia coli with respect to operon structure and regulation. Journal of Bacteriology, 180(3), 722-31.
Römling U, et al. Curli Fibers Are Highly Conserved Between Salmonella Typhimurium and Escherichia Coli With Respect to Operon Structure and Regulation. J Bacteriol. 1998;180(3):722-31. PubMed PMID: 9457880.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Curli fibers are highly conserved between Salmonella typhimurium and Escherichia coli with respect to operon structure and regulation. AU - Römling,U, AU - Bian,Z, AU - Hammar,M, AU - Sierralta,W D, AU - Normark,S, PY - 1998/2/11/pubmed PY - 1998/2/11/medline PY - 1998/2/11/entrez SP - 722 EP - 31 JF - Journal of bacteriology JO - J Bacteriol VL - 180 IS - 3 N2 - Mouse-virulent Salmonella typhimurium strains SR-11 and ATCC 14028-1s express curli fibers, thin aggregative fibers, at ambient temperature on plates as judged by Western blot analysis and electron microscopy. Concomitantly with curli expression, cells develop a rough and dry colony morphology and bind the dye Congo red (called the rdar morphotype). Cloning and characterization of the two divergently transcribed operons required for curli biogenesis, csgBA(C) and csgDEFG, from S. typhimurium SR-11 revealed the same gene order and flanking genes as in Escherichia coli. The divergence of the curli region between S. typhimurium and E. coli at the nucleotide level is above average (22.4%). However, a high level of conservation at the protein level, which ranged from 86% amino acid homology for the fiber subunit CsgA to 99% homology for the lipoprotein CsgG, implies functional constraints on the gene products. Consequently, S. typhimurium genes on low-copy-number plasmids were able to complement respective E. coli mutants, although not always to wild-type levels. rpoS and ompR are required for transcriptional activation of (at least) the csgD promoter. The high degree of conservation at the protein level and the identical regulation patterns in E. coli and S. typhimurium suggest similar roles of curli fibers in the same ecological niche in the two species. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/9457880/Curli_fibers_are_highly_conserved_between_Salmonella_typhimurium_and_Escherichia_coli_with_respect_to_operon_structure_and_regulation_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=9457880 DB - PRIME DP - Unbound Medicine ER -