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Na(+)-K(+)-ATPase expression in alveolar epithelial cells: upregulation of active ion transport by KGF.
Am J Physiol 1998; 274(1):L149-58AJ

Abstract

We evaluated the effects of keratinocyte growth factor (KGF) on alveolar epithelial cell (AEC) active ion transport and on rat epithelial Na channel (rENaC) subunit and Na(+)-K(+)-adenosinetriphosphatase (ATPase) subunit isoform expression using monolayers of AEC grown in primary culture. Rat alveolar type II cells were plated on polycarbonate filters in serum-free medium, and KGF (10 ng/ml) was added to confluent AEC monolayers on day 4 in culture. Exposure of AEC monolayers to KGF on day 4 resulted in dose-dependent increases in short-circuit current (Isc) compared with controls by day 5, with further increases occurring through day 8. Relative Na(+)-K(+)-ATPase alpha 1-subunit mRNA abundance was increased by 41% on days 6 and 8 after exposure to KGF, whereas alpha 2-subunit mRNA remained only marginally detectable in both the absence and presence of KGF. Levels of mRNA for the beta 1-subunit of Na(+)-K(+)-ATPase did not increase, whereas cellular alpha 1- and beta 1-subunit protein increased 70 and 31%, respectively, on day 6. mRNA for alpha-, beta-, and gamma-rENaC all decreased in abundance after treatment with KGF. These results indicate that KGF upregulates active ion transport across AEC monolayers via a KGF-induced increase in Na pumps, primarily due to increased Na(+)-K(+)-ATPase alpha 1-subunit mRNA expression. We conclude that KGF may enhance alveolar fluid clearance after acute lung injury by upregulating Na pump expression and transepithelial Na transport across the alveolar epithelium.

Authors+Show Affiliations

Will Rogers Institute Pulmonary Research Center, Division of Pulmonary and Critical Care Medicine, University of Southern California, Los Angeles 90033, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9458813

Citation

Borok, Z, et al. "Na(+)-K(+)-ATPase Expression in Alveolar Epithelial Cells: Upregulation of Active Ion Transport By KGF." The American Journal of Physiology, vol. 274, no. 1, 1998, pp. L149-58.
Borok Z, Danto SI, Dimen LL, et al. Na(+)-K(+)-ATPase expression in alveolar epithelial cells: upregulation of active ion transport by KGF. Am J Physiol. 1998;274(1):L149-58.
Borok, Z., Danto, S. I., Dimen, L. L., Zhang, X. L., & Lubman, R. L. (1998). Na(+)-K(+)-ATPase expression in alveolar epithelial cells: upregulation of active ion transport by KGF. The American Journal of Physiology, 274(1), pp. L149-58. doi:10.1152/ajplung.1998.274.1.L149.
Borok Z, et al. Na(+)-K(+)-ATPase Expression in Alveolar Epithelial Cells: Upregulation of Active Ion Transport By KGF. Am J Physiol. 1998;274(1):L149-58. PubMed PMID: 9458813.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Na(+)-K(+)-ATPase expression in alveolar epithelial cells: upregulation of active ion transport by KGF. AU - Borok,Z, AU - Danto,S I, AU - Dimen,L L, AU - Zhang,X L, AU - Lubman,R L, PY - 1998/2/12/pubmed PY - 1998/2/12/medline PY - 1998/2/12/entrez SP - L149 EP - 58 JF - The American journal of physiology JO - Am. J. Physiol. VL - 274 IS - 1 N2 - We evaluated the effects of keratinocyte growth factor (KGF) on alveolar epithelial cell (AEC) active ion transport and on rat epithelial Na channel (rENaC) subunit and Na(+)-K(+)-adenosinetriphosphatase (ATPase) subunit isoform expression using monolayers of AEC grown in primary culture. Rat alveolar type II cells were plated on polycarbonate filters in serum-free medium, and KGF (10 ng/ml) was added to confluent AEC monolayers on day 4 in culture. Exposure of AEC monolayers to KGF on day 4 resulted in dose-dependent increases in short-circuit current (Isc) compared with controls by day 5, with further increases occurring through day 8. Relative Na(+)-K(+)-ATPase alpha 1-subunit mRNA abundance was increased by 41% on days 6 and 8 after exposure to KGF, whereas alpha 2-subunit mRNA remained only marginally detectable in both the absence and presence of KGF. Levels of mRNA for the beta 1-subunit of Na(+)-K(+)-ATPase did not increase, whereas cellular alpha 1- and beta 1-subunit protein increased 70 and 31%, respectively, on day 6. mRNA for alpha-, beta-, and gamma-rENaC all decreased in abundance after treatment with KGF. These results indicate that KGF upregulates active ion transport across AEC monolayers via a KGF-induced increase in Na pumps, primarily due to increased Na(+)-K(+)-ATPase alpha 1-subunit mRNA expression. We conclude that KGF may enhance alveolar fluid clearance after acute lung injury by upregulating Na pump expression and transepithelial Na transport across the alveolar epithelium. SN - 0002-9513 UR - https://www.unboundmedicine.com/medline/citation/9458813/Na_+__K_+__ATPase_expression_in_alveolar_epithelial_cells:_upregulation_of_active_ion_transport_by_KGF_ L2 - http://www.physiology.org/doi/full/10.1152/ajplung.1998.274.1.L149?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -