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Protein and Mg(2+)-induced conformational changes in the S15 binding site of 16 S ribosomal RNA.
J Mol Biol. 1998 Jan 23; 275(3):453-64.JM

Abstract

The Bacillus stearothermophilus ribosomal protein S15 binds to the central domain of the 16 S rRNA inducing a conformational change in a three-way helical junction. To understand the nature of this conformational change, extended-helical junctions were prepared to examine the effects of S15 or Mg2+ binding on the relative helical orientation using native gel electrophoretic mobility and transient electric birefringence. The free junction is planar with approximately 120 degrees interhelical angles, whereas S15 and Mg2+ yield a junction conformation that remains planar in which two helices, 21 and 22, become colinear and the third, helix 20, forms a 60 degrees angle with respect to helix 22. This conformational change is thought to be important for directing the assembly of the central domain of the 30 S ribosomal subunit.

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Authors+Show Affiliations

Orr JW
Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139, USA.
Hagerman PJ
No affiliation info available
Williamson JR
No affiliation info available

MeSH

Base SequenceBinding SitesElectrophoresis, Polyacrylamide GelGeobacillus stearothermophilusLigandsMagnesiumModels, MolecularMolecular Sequence DataNucleic Acid ConformationProtein BindingRNA, Ribosomal, 16SRNA-Binding ProteinsRibosomal Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9466923

Citation

Orr, J W., et al. "Protein and Mg(2+)-induced Conformational Changes in the S15 Binding Site of 16 S Ribosomal RNA." Journal of Molecular Biology, vol. 275, no. 3, 1998, pp. 453-64.
Orr JW, Hagerman PJ, Williamson JR. Protein and Mg(2+)-induced conformational changes in the S15 binding site of 16 S ribosomal RNA. J Mol Biol. 1998;275(3):453-64.
Orr, J. W., Hagerman, P. J., & Williamson, J. R. (1998). Protein and Mg(2+)-induced conformational changes in the S15 binding site of 16 S ribosomal RNA. Journal of Molecular Biology, 275(3), 453-64.
Orr JW, Hagerman PJ, Williamson JR. Protein and Mg(2+)-induced Conformational Changes in the S15 Binding Site of 16 S Ribosomal RNA. J Mol Biol. 1998 Jan 23;275(3):453-64. PubMed PMID: 9466923.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protein and Mg(2+)-induced conformational changes in the S15 binding site of 16 S ribosomal RNA. AU - Orr,J W, AU - Hagerman,P J, AU - Williamson,J R, PY - 1998/2/19/pubmed PY - 1998/2/19/medline PY - 1998/2/19/entrez SP - 453 EP - 64 JF - Journal of molecular biology JO - J Mol Biol VL - 275 IS - 3 N2 - The Bacillus stearothermophilus ribosomal protein S15 binds to the central domain of the 16 S rRNA inducing a conformational change in a three-way helical junction. To understand the nature of this conformational change, extended-helical junctions were prepared to examine the effects of S15 or Mg2+ binding on the relative helical orientation using native gel electrophoretic mobility and transient electric birefringence. The free junction is planar with approximately 120 degrees interhelical angles, whereas S15 and Mg2+ yield a junction conformation that remains planar in which two helices, 21 and 22, become colinear and the third, helix 20, forms a 60 degrees angle with respect to helix 22. This conformational change is thought to be important for directing the assembly of the central domain of the 30 S ribosomal subunit. SN - 0022-2836 UR - https://www.unboundmedicine.com/medline/citation/9466923/Protein_and_Mg_2+__induced_conformational_changes_in_the_S15_binding_site_of_16_S_ribosomal_RNA_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2836(97)91489-3 DB - PRIME DP - Unbound Medicine ER -