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Immortalized cells with no detectable telomerase activity. A review.
Biochemistry (Mosc). 1997 Nov; 62(11):1254-62.B

Abstract

Immortalization of human cells in culture is usually associated with expression of telomerase activity. In some cases, however, no telomerase activity is detectable even though comparison of the terminal restriction fragment (TRF) pattern before and after immortalization shows that lengthening of telomeres has occurred. The extreme heterogeneity in telomere length and the differences in the dynamics of telomere maintenance in telomerase-negative cell lines compared to telomerase-positive cell lines indicate that these cells have utilized one or more alternative mechanisms for lengthening of telomeres (ALT). All telomerase-negative immortalized cell lines examined to date show evidence of ALT activity, consistent with the hypothesis that telomere maintenance either by telomerase or by ALT is required for immortalization. The nature of the ALT mechanism(s) is currently unknown, but studies of telomere dynamics in an ALT cell line containing a marker just proximal to the telomeric sequences show gradual shortening of the telomere followed by rapid elongation. This is consistent with a non-reciprocal recombinational mechanism similar to that found in telomerase-defective mutant yeast strains.

Authors+Show Affiliations

Children's Medical Research Institute, Sydney, NSW, Australia. rreddel@mail.usyd.edu.auNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
Review

Language

eng

PubMed ID

9467849

Citation

Reddel, R R., et al. "Immortalized Cells With No Detectable Telomerase Activity. a Review." Biochemistry. Biokhimiia, vol. 62, no. 11, 1997, pp. 1254-62.
Reddel RR, Bryan TM, Murnane JP. Immortalized cells with no detectable telomerase activity. A review. Biochemistry Mosc. 1997;62(11):1254-62.
Reddel, R. R., Bryan, T. M., & Murnane, J. P. (1997). Immortalized cells with no detectable telomerase activity. A review. Biochemistry. Biokhimiia, 62(11), 1254-62.
Reddel RR, Bryan TM, Murnane JP. Immortalized Cells With No Detectable Telomerase Activity. a Review. Biochemistry Mosc. 1997;62(11):1254-62. PubMed PMID: 9467849.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immortalized cells with no detectable telomerase activity. A review. AU - Reddel,R R, AU - Bryan,T M, AU - Murnane,J P, PY - 1998/2/19/pubmed PY - 1998/2/19/medline PY - 1998/2/19/entrez SP - 1254 EP - 62 JF - Biochemistry. Biokhimiia JO - Biochemistry Mosc. VL - 62 IS - 11 N2 - Immortalization of human cells in culture is usually associated with expression of telomerase activity. In some cases, however, no telomerase activity is detectable even though comparison of the terminal restriction fragment (TRF) pattern before and after immortalization shows that lengthening of telomeres has occurred. The extreme heterogeneity in telomere length and the differences in the dynamics of telomere maintenance in telomerase-negative cell lines compared to telomerase-positive cell lines indicate that these cells have utilized one or more alternative mechanisms for lengthening of telomeres (ALT). All telomerase-negative immortalized cell lines examined to date show evidence of ALT activity, consistent with the hypothesis that telomere maintenance either by telomerase or by ALT is required for immortalization. The nature of the ALT mechanism(s) is currently unknown, but studies of telomere dynamics in an ALT cell line containing a marker just proximal to the telomeric sequences show gradual shortening of the telomere followed by rapid elongation. This is consistent with a non-reciprocal recombinational mechanism similar to that found in telomerase-defective mutant yeast strains. SN - 0006-2979 UR - https://www.unboundmedicine.com/medline/citation/9467849/Immortalized_cells_with_no_detectable_telomerase_activity__A_review_ L2 - https://www.lens.org/lens/search?q=citation_id:9467849 DB - PRIME DP - Unbound Medicine ER -