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Studies on the structure and the solution conformation of an acidic extracellular polysaccharide isolated from Bradyrhizobium.
Carbohydr Res. 1997 Nov 28; 304(3-4):209-17.CR

Abstract

The structure of an acidic extracellular polysaccharide isolated from Bradyrhizobium (Chamaecytisus proliferus) has been elucidated by hydrolysis, methylation analysis, and 1D and 2D 1H- and 13C-NMR spectroscopy of the complete polysaccharide. The NMR spectrum showed that microheterogeneity was present due to the minor existence of a variety of O-acetyl groups. Thus, a deacetylated sample was prepared by alkaline treatment which was then fully analysed. The deacetylated polysaccharide has the following sequence: -->3)-[alpha-D-Galp-(1-->6)-alpha-D-Glcp-(1-->3)-beta-D-Glcp-(1-->3)- alpha-D-GalpA-(1-->3)-alpha-D-Manp-(1--> The sample is partially O-methylated at position 4 of the alpha-D-Galp-(1-->6) unit. In addition, the same moiety of the native sample is also partially and heterogeneously O-acetylated. The conformational features of the deacetylated sample have been evaluated by molecular mechanics and dynamics calculations and NOE spectroscopy. The results indicate that the polysaccharide may adopt a variety of three dimensional shapes, and that there is a fair agreement between the NMR-derived distances and those provided by the calculations

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Authors+Show Affiliations

Poveda A
Servicio Interdepartamental de Investigación, Universidad Autonoma de Madrid, Spain.
Santamaría M
No affiliation info available
Bernabé M
No affiliation info available
Prieto A
No affiliation info available
Bruix M
No affiliation info available
Corzo J
No affiliation info available
Jiménez-Barbero J
No affiliation info available

MeSH

Carbohydrate ConformationCarbohydrate SequenceMethylationModels, MolecularMolecular Sequence DataMolecular WeightNuclear Magnetic Resonance, BiomolecularOligosaccharidesPolysaccharides, BacterialRhizobiaceaeSolutions

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9468626

Citation

Poveda, A, et al. "Studies On the Structure and the Solution Conformation of an Acidic Extracellular Polysaccharide Isolated From Bradyrhizobium." Carbohydrate Research, vol. 304, no. 3-4, 1997, pp. 209-17.
Poveda A, Santamaría M, Bernabé M, et al. Studies on the structure and the solution conformation of an acidic extracellular polysaccharide isolated from Bradyrhizobium. Carbohydr Res. 1997;304(3-4):209-17.
Poveda, A., Santamaría, M., Bernabé, M., Prieto, A., Bruix, M., Corzo, J., & Jiménez-Barbero, J. (1997). Studies on the structure and the solution conformation of an acidic extracellular polysaccharide isolated from Bradyrhizobium. Carbohydrate Research, 304(3-4), 209-17.
Poveda A, et al. Studies On the Structure and the Solution Conformation of an Acidic Extracellular Polysaccharide Isolated From Bradyrhizobium. Carbohydr Res. 1997 Nov 28;304(3-4):209-17. PubMed PMID: 9468626.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Studies on the structure and the solution conformation of an acidic extracellular polysaccharide isolated from Bradyrhizobium. AU - Poveda,A, AU - Santamaría,M, AU - Bernabé,M, AU - Prieto,A, AU - Bruix,M, AU - Corzo,J, AU - Jiménez-Barbero,J, PY - 1998/2/20/pubmed PY - 1998/2/20/medline PY - 1998/2/20/entrez SP - 209 EP - 17 JF - Carbohydrate research JO - Carbohydr Res VL - 304 IS - 3-4 N2 - The structure of an acidic extracellular polysaccharide isolated from Bradyrhizobium (Chamaecytisus proliferus) has been elucidated by hydrolysis, methylation analysis, and 1D and 2D 1H- and 13C-NMR spectroscopy of the complete polysaccharide. The NMR spectrum showed that microheterogeneity was present due to the minor existence of a variety of O-acetyl groups. Thus, a deacetylated sample was prepared by alkaline treatment which was then fully analysed. The deacetylated polysaccharide has the following sequence: -->3)-[alpha-D-Galp-(1-->6)-alpha-D-Glcp-(1-->3)-beta-D-Glcp-(1-->3)- alpha-D-GalpA-(1-->3)-alpha-D-Manp-(1--> The sample is partially O-methylated at position 4 of the alpha-D-Galp-(1-->6) unit. In addition, the same moiety of the native sample is also partially and heterogeneously O-acetylated. The conformational features of the deacetylated sample have been evaluated by molecular mechanics and dynamics calculations and NOE spectroscopy. The results indicate that the polysaccharide may adopt a variety of three dimensional shapes, and that there is a fair agreement between the NMR-derived distances and those provided by the calculations SN - 0008-6215 UR - https://www.unboundmedicine.com/medline/citation/9468626/Studies_on_the_structure_and_the_solution_conformation_of_an_acidic_extracellular_polysaccharide_isolated_from_Bradyrhizobium_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0008-6215(97)00237-1 DB - PRIME DP - Unbound Medicine ER -