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Comparison of interleukin-11 and epidermal growth factor on residual small intestine after massive small bowel resection.
J Pediatr Surg. 1998 Jan; 33(1):24-9.JP

Abstract

BACKGROUND/PURPOSE

Interleukin-11 (IL-11) is a multifunctional cytokine derived from bone marrow, which has a trophic effect on small bowel epithelium. This study compares the effects of IL-11 with epidermal growth factor (EGF), a growth factor known to enhance small bowel adaptation.

METHODS

Forty Sprague-Dawley rats (90 to 100 g) underwent an 85% mid-small bowel resection with primary anastomosis on day 0. Rats were divided into four treatment groups: controls (group I) received bovine serum albumin (BSA), group II received IL-11, 125 microg/kg subcutaneously (SC) twice daily, group III received EGF, 0.10 microg/g SC bid, and group IV received EGF and IL-11 in the above doses. Half of the animals (five per group) were killed on day 4 of therapy, and the rest were killed on day 8. Animals were evaluated for weight, mucosal length, and bowel wall muscle thickness on days 4 and 8, and expression of proliferating cell nuclear antigen (PCNA) in intestinal crypt and smooth muscle cells on day 8.

RESULTS

There were two deaths; both were 8-day controls. Body weight was similar at day 4 and day 8. Mucosal thickness in groups II (IL-11) and group IV (IL-11 and EGF) was significantly increased at day 4 and 8 when compared with controls (group I) and EGF (group III, P < .001). Muscle thickness was significantly increased in the EGF and combined group IV compared with the BSA controls and IL-11 groups (P < .001). Thirty-two percent of the mucosal crypt cells in Group I stained positive for PCNA, whereas 51%, 53%, and 60% stained positive in groups II (IL-11), III (EGF), and IV (IL-11 and EGF), respectively. In groups I and II, 2% and 1.7% of the myocytes stained positive for PCNA, whereas 11.2% and 5.2% of the myocytes in group III and IV stained positive.

CONCLUSIONS

These data suggest that IL-11 has a trophic effect on small intestinal enterocytes, causing cell proliferation and increased mucosal thickness. EGF has a more generalized effect on intestine causing proliferation of both enterocytes and myocytes. IL-11, with or without EGF, may be a useful adjunct in instances of short bowel syndrome.

Authors+Show Affiliations

Department of Surgery, Herman B. Wells Center for Pediatric Research and the Howard Hughes Medical Institute, Indiana University School of Medicine, Indianapolis, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

9473093

Citation

Fiore, N F., et al. "Comparison of Interleukin-11 and Epidermal Growth Factor On Residual Small Intestine After Massive Small Bowel Resection." Journal of Pediatric Surgery, vol. 33, no. 1, 1998, pp. 24-9.
Fiore NF, Ledniczky G, Liu Q, et al. Comparison of interleukin-11 and epidermal growth factor on residual small intestine after massive small bowel resection. J Pediatr Surg. 1998;33(1):24-9.
Fiore, N. F., Ledniczky, G., Liu, Q., Orazi, A., Du, X., Williams, D. A., & Grosfeld, J. L. (1998). Comparison of interleukin-11 and epidermal growth factor on residual small intestine after massive small bowel resection. Journal of Pediatric Surgery, 33(1), 24-9.
Fiore NF, et al. Comparison of Interleukin-11 and Epidermal Growth Factor On Residual Small Intestine After Massive Small Bowel Resection. J Pediatr Surg. 1998;33(1):24-9. PubMed PMID: 9473093.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of interleukin-11 and epidermal growth factor on residual small intestine after massive small bowel resection. AU - Fiore,N F, AU - Ledniczky,G, AU - Liu,Q, AU - Orazi,A, AU - Du,X, AU - Williams,D A, AU - Grosfeld,J L, PY - 1998/2/24/pubmed PY - 1998/2/24/medline PY - 1998/2/24/entrez SP - 24 EP - 9 JF - Journal of pediatric surgery JO - J Pediatr Surg VL - 33 IS - 1 N2 - BACKGROUND/PURPOSE: Interleukin-11 (IL-11) is a multifunctional cytokine derived from bone marrow, which has a trophic effect on small bowel epithelium. This study compares the effects of IL-11 with epidermal growth factor (EGF), a growth factor known to enhance small bowel adaptation. METHODS: Forty Sprague-Dawley rats (90 to 100 g) underwent an 85% mid-small bowel resection with primary anastomosis on day 0. Rats were divided into four treatment groups: controls (group I) received bovine serum albumin (BSA), group II received IL-11, 125 microg/kg subcutaneously (SC) twice daily, group III received EGF, 0.10 microg/g SC bid, and group IV received EGF and IL-11 in the above doses. Half of the animals (five per group) were killed on day 4 of therapy, and the rest were killed on day 8. Animals were evaluated for weight, mucosal length, and bowel wall muscle thickness on days 4 and 8, and expression of proliferating cell nuclear antigen (PCNA) in intestinal crypt and smooth muscle cells on day 8. RESULTS: There were two deaths; both were 8-day controls. Body weight was similar at day 4 and day 8. Mucosal thickness in groups II (IL-11) and group IV (IL-11 and EGF) was significantly increased at day 4 and 8 when compared with controls (group I) and EGF (group III, P < .001). Muscle thickness was significantly increased in the EGF and combined group IV compared with the BSA controls and IL-11 groups (P < .001). Thirty-two percent of the mucosal crypt cells in Group I stained positive for PCNA, whereas 51%, 53%, and 60% stained positive in groups II (IL-11), III (EGF), and IV (IL-11 and EGF), respectively. In groups I and II, 2% and 1.7% of the myocytes stained positive for PCNA, whereas 11.2% and 5.2% of the myocytes in group III and IV stained positive. CONCLUSIONS: These data suggest that IL-11 has a trophic effect on small intestinal enterocytes, causing cell proliferation and increased mucosal thickness. EGF has a more generalized effect on intestine causing proliferation of both enterocytes and myocytes. IL-11, with or without EGF, may be a useful adjunct in instances of short bowel syndrome. SN - 0022-3468 UR - https://www.unboundmedicine.com/medline/citation/9473093/Comparison_of_interleukin_11_and_epidermal_growth_factor_on_residual_small_intestine_after_massive_small_bowel_resection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-3468(98)90354-2 DB - PRIME DP - Unbound Medicine ER -