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Glutamine synthetase activity and HSP70 levels in cultured rat astrocytes: effect of 1-octadecyl-2-methyl-rac-glycero-3-phosphocholine.
Brain Res. 1998 Feb 02; 783(1):143-50.BR

Abstract

The ether lipid 1-octadecyl-2-methyl-rac-glycero-3-phosphocholine (ET-18-OCH3) is a membrane interactive drug selectively cytotoxic toward neoplastic cells compared to normal cells. It induces apoptosis in human leukemic HL-60, T-lymphoid and in U937 myeloid cell lines and stimulates NO biosynthesis in cultured rat astrocytes. We have found a double action of ET-18-OCH3 in astrocytes which, at low doses, promotes a moderate induction of heat shock proteins of 70 kDa (HSP70) and the increase of glutamine synthetase (GS) activity. Conversely, at high doses, the drug shows toxic effects on astrocytes inducing decrease in GS activity, low molecular weight DNA formation, and release of lactic dehydrogenase (LDH) in the culture medium. Its analog compound platelet-activating factor (PAF) shares some of these biological aspects.

Authors+Show Affiliations

Institute of Biochemistry, Faculty of Pharmacy, University of Catania, Catania, Italy. chimbio@ictuniv.unict.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9479063

Citation

Renis, M, et al. "Glutamine Synthetase Activity and HSP70 Levels in Cultured Rat Astrocytes: Effect of 1-octadecyl-2-methyl-rac-glycero-3-phosphocholine." Brain Research, vol. 783, no. 1, 1998, pp. 143-50.
Renis M, Cardile V, Russo A, et al. Glutamine synthetase activity and HSP70 levels in cultured rat astrocytes: effect of 1-octadecyl-2-methyl-rac-glycero-3-phosphocholine. Brain Res. 1998;783(1):143-50.
Renis, M., Cardile, V., Russo, A., Campisi, A., & Collovà, F. (1998). Glutamine synthetase activity and HSP70 levels in cultured rat astrocytes: effect of 1-octadecyl-2-methyl-rac-glycero-3-phosphocholine. Brain Research, 783(1), 143-50.
Renis M, et al. Glutamine Synthetase Activity and HSP70 Levels in Cultured Rat Astrocytes: Effect of 1-octadecyl-2-methyl-rac-glycero-3-phosphocholine. Brain Res. 1998 Feb 2;783(1):143-50. PubMed PMID: 9479063.
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TY - JOUR T1 - Glutamine synthetase activity and HSP70 levels in cultured rat astrocytes: effect of 1-octadecyl-2-methyl-rac-glycero-3-phosphocholine. AU - Renis,M, AU - Cardile,V, AU - Russo,A, AU - Campisi,A, AU - Collovà,F, PY - 1998/5/2/pubmed PY - 1998/5/2/medline PY - 1998/5/2/entrez SP - 143 EP - 50 JF - Brain research JO - Brain Res VL - 783 IS - 1 N2 - The ether lipid 1-octadecyl-2-methyl-rac-glycero-3-phosphocholine (ET-18-OCH3) is a membrane interactive drug selectively cytotoxic toward neoplastic cells compared to normal cells. It induces apoptosis in human leukemic HL-60, T-lymphoid and in U937 myeloid cell lines and stimulates NO biosynthesis in cultured rat astrocytes. We have found a double action of ET-18-OCH3 in astrocytes which, at low doses, promotes a moderate induction of heat shock proteins of 70 kDa (HSP70) and the increase of glutamine synthetase (GS) activity. Conversely, at high doses, the drug shows toxic effects on astrocytes inducing decrease in GS activity, low molecular weight DNA formation, and release of lactic dehydrogenase (LDH) in the culture medium. Its analog compound platelet-activating factor (PAF) shares some of these biological aspects. SN - 0006-8993 UR - https://www.unboundmedicine.com/medline/citation/9479063/Glutamine_synthetase_activity_and_HSP70_levels_in_cultured_rat_astrocytes:_effect_of_1_octadecyl_2_methyl_rac_glycero_3_phosphocholine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(97)01321-8 DB - PRIME DP - Unbound Medicine ER -