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High-performance capillary electrophoretic separation of carbohydrates with indirect UV detection using diethylamine and borate as electrolyte additives.
J Capillary Electrophor. 1997 May-Jun; 4(3):97-103.JC

Abstract

The separation of underivatized carbohydrates was investigated using capillary zone electrophoresis with indirect UV detection based on sorbic acid or riboflavin. In comparison with sodium hydroxide, the separation of carbohydrates was greatly improved using diethylamine (DEA) as an electrolyte additive. Since the conductivity of DEA is much lower than that of sodium hydroxide, a stable baseline and a 3-5 times improvement in separation efficiency was observed at a pH as high as 12.6. It was found that DEA reduced the electrophoretic mobility of carbohydrates by formation of ion-pairs, and also reduced the electroosmotic flow by masking the silanol groups at the capillary surface. Many monosaccharides, including glucose, rhamnose, mannose, and fructose, which have very similar pKa values, were completely separated using 5% DEA at pH 12.6. The separation of di- and trisaccharides using indirect detection with a borate buffer at pH 9.5 was also investigated. The separation of disaccharides was greatly improved with borate complexation using riboflavin as a background electrolyte (BGE) over the sodium hydroxide and DEA electrolyte system with sorbic acid as BGE. The resolution of carbohydrates increased as pH increased, and sucrose, trehalose, raffinose, and maltotriose were successfully separated at pH 11.8.

Authors+Show Affiliations

Liu Y
Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA.
Shu C
No affiliation info available
Lamb JD
No affiliation info available

MeSH

Boric AcidsCarbohydratesDiethylaminesElectrolytesElectrophoresis, CapillarySensitivity and SpecificityUltraviolet Rays

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9484655

Citation

Liu, Y, et al. "High-performance Capillary Electrophoretic Separation of Carbohydrates With Indirect UV Detection Using Diethylamine and Borate as Electrolyte Additives." Journal of Capillary Electrophoresis, vol. 4, no. 3, 1997, pp. 97-103.
Liu Y, Shu C, Lamb JD. High-performance capillary electrophoretic separation of carbohydrates with indirect UV detection using diethylamine and borate as electrolyte additives. J Capillary Electrophor. 1997;4(3):97-103.
Liu, Y., Shu, C., & Lamb, J. D. (1997). High-performance capillary electrophoretic separation of carbohydrates with indirect UV detection using diethylamine and borate as electrolyte additives. Journal of Capillary Electrophoresis, 4(3), 97-103.
Liu Y, Shu C, Lamb JD. High-performance Capillary Electrophoretic Separation of Carbohydrates With Indirect UV Detection Using Diethylamine and Borate as Electrolyte Additives. J Capillary Electrophor. 1997 May-Jun;4(3):97-103. PubMed PMID: 9484655.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-performance capillary electrophoretic separation of carbohydrates with indirect UV detection using diethylamine and borate as electrolyte additives. AU - Liu,Y, AU - Shu,C, AU - Lamb,J D, PY - 1997/5/1/pubmed PY - 1998/3/4/medline PY - 1997/5/1/entrez SP - 97 EP - 103 JF - Journal of capillary electrophoresis JO - J Capillary Electrophor VL - 4 IS - 3 N2 - The separation of underivatized carbohydrates was investigated using capillary zone electrophoresis with indirect UV detection based on sorbic acid or riboflavin. In comparison with sodium hydroxide, the separation of carbohydrates was greatly improved using diethylamine (DEA) as an electrolyte additive. Since the conductivity of DEA is much lower than that of sodium hydroxide, a stable baseline and a 3-5 times improvement in separation efficiency was observed at a pH as high as 12.6. It was found that DEA reduced the electrophoretic mobility of carbohydrates by formation of ion-pairs, and also reduced the electroosmotic flow by masking the silanol groups at the capillary surface. Many monosaccharides, including glucose, rhamnose, mannose, and fructose, which have very similar pKa values, were completely separated using 5% DEA at pH 12.6. The separation of di- and trisaccharides using indirect detection with a borate buffer at pH 9.5 was also investigated. The separation of disaccharides was greatly improved with borate complexation using riboflavin as a background electrolyte (BGE) over the sodium hydroxide and DEA electrolyte system with sorbic acid as BGE. The resolution of carbohydrates increased as pH increased, and sucrose, trehalose, raffinose, and maltotriose were successfully separated at pH 11.8. SN - 1079-5383 UR - https://www.unboundmedicine.com/medline/citation/9484655/High_performance_capillary_electrophoretic_separation_of_carbohydrates_with_indirect_UV_detection_using_diethylamine_and_borate_as_electrolyte_additives_ DB - PRIME DP - Unbound Medicine ER -