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Effect of nitroglycerin and long-term electrical stimulation on nitrergic relaxation in the pig gastric fundus.
Br J Pharmacol. 1998 Jan; 123(1):143-9.BJ

Abstract

1. The effect of incubation with the nitric oxide (NO) donor nitroglycerin and of long-term electrical stimulation on relaxations induced by non-adrenergic, non-cholinergic nerve stimulation, exogenous NO, vasoactive intestinal polypeptide (VIP) and lemakalim was investigated in the pig gastric fundus. 2. In physiological salt solution containing 10(-6) M atropine and 4 x 10(-6) M guanethidine, electrical field stimulation (40 V, 0.1 ms, 0.5-8 Hz) for periods of 10 s at 5 min intervals (train stimulation) and administration of NO (2 x 10(-6)-10(-4) M) at 5 min intervals (NO boli) induced frequency- and concentration-dependent transient relaxations, respectively. Continuous electrical field stimulation with stepwise increase of the frequency (0.5-8 Hz, cumulative stimulation) induced frequency-dependent sustained relaxations. VIP (10(-7) M), lemakalim (10(-5) M) and an infusion of NO induced a sustained relaxation. 3. Pretreatment for 30 min with 5 x 10(-4) M nitroglycerin reduced the relaxations induced by train and cumulative stimulation, but also the relaxant responses to NO, both when given in boli or as an infusion. The relaxations to VIP and lemakalim were not influenced by pretreatment with nitroglycerin. 4. Long-term electrical stimulation at 4 Hz for 40 min induced a sustained relaxation of the tissues. Administration of 3 x 10(-4) M N(G)-nitro-L-arginine methyl ester after 10, 20 or 30 min reversed the relaxation to a similar extent (approximately 70%). Previous long-term electrical stimulation at 4 Hz for 30 min did not affect the responses to stimulation, NO and VIP. 5. These results illustrate that nitroglycerin can induce a postjunctional tolerance to nitrergic stimuli in the pig gastric fundus but evidence for a prejunctional inhibition of neuronal NO synthase by NO was not obtained.

Authors+Show Affiliations

Heymans Institute of Pharmacology, University of Gent Medical School, Belgium.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9484865

Citation

Lefebvre, R A., and K Vandekerckhove. "Effect of Nitroglycerin and Long-term Electrical Stimulation On Nitrergic Relaxation in the Pig Gastric Fundus." British Journal of Pharmacology, vol. 123, no. 1, 1998, pp. 143-9.
Lefebvre RA, Vandekerckhove K. Effect of nitroglycerin and long-term electrical stimulation on nitrergic relaxation in the pig gastric fundus. Br J Pharmacol. 1998;123(1):143-9.
Lefebvre, R. A., & Vandekerckhove, K. (1998). Effect of nitroglycerin and long-term electrical stimulation on nitrergic relaxation in the pig gastric fundus. British Journal of Pharmacology, 123(1), 143-9.
Lefebvre RA, Vandekerckhove K. Effect of Nitroglycerin and Long-term Electrical Stimulation On Nitrergic Relaxation in the Pig Gastric Fundus. Br J Pharmacol. 1998;123(1):143-9. PubMed PMID: 9484865.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of nitroglycerin and long-term electrical stimulation on nitrergic relaxation in the pig gastric fundus. AU - Lefebvre,R A, AU - Vandekerckhove,K, PY - 1998/3/4/pubmed PY - 1998/3/4/medline PY - 1998/3/4/entrez SP - 143 EP - 9 JF - British journal of pharmacology JO - Br J Pharmacol VL - 123 IS - 1 N2 - 1. The effect of incubation with the nitric oxide (NO) donor nitroglycerin and of long-term electrical stimulation on relaxations induced by non-adrenergic, non-cholinergic nerve stimulation, exogenous NO, vasoactive intestinal polypeptide (VIP) and lemakalim was investigated in the pig gastric fundus. 2. In physiological salt solution containing 10(-6) M atropine and 4 x 10(-6) M guanethidine, electrical field stimulation (40 V, 0.1 ms, 0.5-8 Hz) for periods of 10 s at 5 min intervals (train stimulation) and administration of NO (2 x 10(-6)-10(-4) M) at 5 min intervals (NO boli) induced frequency- and concentration-dependent transient relaxations, respectively. Continuous electrical field stimulation with stepwise increase of the frequency (0.5-8 Hz, cumulative stimulation) induced frequency-dependent sustained relaxations. VIP (10(-7) M), lemakalim (10(-5) M) and an infusion of NO induced a sustained relaxation. 3. Pretreatment for 30 min with 5 x 10(-4) M nitroglycerin reduced the relaxations induced by train and cumulative stimulation, but also the relaxant responses to NO, both when given in boli or as an infusion. The relaxations to VIP and lemakalim were not influenced by pretreatment with nitroglycerin. 4. Long-term electrical stimulation at 4 Hz for 40 min induced a sustained relaxation of the tissues. Administration of 3 x 10(-4) M N(G)-nitro-L-arginine methyl ester after 10, 20 or 30 min reversed the relaxation to a similar extent (approximately 70%). Previous long-term electrical stimulation at 4 Hz for 30 min did not affect the responses to stimulation, NO and VIP. 5. These results illustrate that nitroglycerin can induce a postjunctional tolerance to nitrergic stimuli in the pig gastric fundus but evidence for a prejunctional inhibition of neuronal NO synthase by NO was not obtained. SN - 0007-1188 UR - https://www.unboundmedicine.com/medline/citation/9484865/Effect_of_nitroglycerin_and_long_term_electrical_stimulation_on_nitrergic_relaxation_in_the_pig_gastric_fundus_ L2 - https://doi.org/10.1038/sj.bjp.0701582 DB - PRIME DP - Unbound Medicine ER -