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Effect of heat inactivation of serum on Bordetella pertussis antibody determination by enzyme-linked immunosorbent assay.
Diagn Microbiol Infect Dis. 1998 Jan; 30(1):21-4.DM

Abstract

The effect of heat inactivation on Bordetella pertussis antibodies determined by enzyme-linked immunosorbent assay (ELISA) was studied. Sera were heated at increasing temperatures (from 30 to 50 degrees C at 5 degrees C increments and from 52 to 70 degrees C at 2 degrees C increments). Between 30 and 50 degrees C, no significant differences were observed in immunoglobin G (IgG) antibodies to pertussis toxin (PT). From 50 to 56 degrees C the antibody values were twofold higher than those of uninactivated sera; at 64 degrees C the values were 3.6- to 9.1-fold higher. The increase in PT IgG antibody values was more pronounced in sera with low antibody values. ELISA antibody values of sera from a vaccine trial were determined in unheated and heat inactivated sera. The geometric mean value (GMV) of the heat inactivated samples was 3.2 times the geometric mean value of the uninactivated sera. ELISA IgG antibodies to filamentous hemagglutinin, fimbriae-2, and pertactin were studied, and the values of heat inactivated sera did not differ significantly from the values of the uninactivated sera. Our findings indicate that heat inactivation of sera leads to higher, variable, and false-positive PT and IgG values.

Authors+Show Affiliations

Department of Pediatrics, UCLA School of Medicine 90095, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Clinical Trial, Phase II
Journal Article
Multicenter Study
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9488826

Citation

Lopez, A L., et al. "Effect of Heat Inactivation of Serum On Bordetella Pertussis Antibody Determination By Enzyme-linked Immunosorbent Assay." Diagnostic Microbiology and Infectious Disease, vol. 30, no. 1, 1998, pp. 21-4.
Lopez AL, Pineda E, Garakian A, et al. Effect of heat inactivation of serum on Bordetella pertussis antibody determination by enzyme-linked immunosorbent assay. Diagn Microbiol Infect Dis. 1998;30(1):21-4.
Lopez, A. L., Pineda, E., Garakian, A., & Cherry, J. D. (1998). Effect of heat inactivation of serum on Bordetella pertussis antibody determination by enzyme-linked immunosorbent assay. Diagnostic Microbiology and Infectious Disease, 30(1), 21-4.
Lopez AL, et al. Effect of Heat Inactivation of Serum On Bordetella Pertussis Antibody Determination By Enzyme-linked Immunosorbent Assay. Diagn Microbiol Infect Dis. 1998;30(1):21-4. PubMed PMID: 9488826.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of heat inactivation of serum on Bordetella pertussis antibody determination by enzyme-linked immunosorbent assay. AU - Lopez,A L, AU - Pineda,E, AU - Garakian,A, AU - Cherry,J D, PY - 1998/3/7/pubmed PY - 1998/3/7/medline PY - 1998/3/7/entrez SP - 21 EP - 4 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 30 IS - 1 N2 - The effect of heat inactivation on Bordetella pertussis antibodies determined by enzyme-linked immunosorbent assay (ELISA) was studied. Sera were heated at increasing temperatures (from 30 to 50 degrees C at 5 degrees C increments and from 52 to 70 degrees C at 2 degrees C increments). Between 30 and 50 degrees C, no significant differences were observed in immunoglobin G (IgG) antibodies to pertussis toxin (PT). From 50 to 56 degrees C the antibody values were twofold higher than those of uninactivated sera; at 64 degrees C the values were 3.6- to 9.1-fold higher. The increase in PT IgG antibody values was more pronounced in sera with low antibody values. ELISA antibody values of sera from a vaccine trial were determined in unheated and heat inactivated sera. The geometric mean value (GMV) of the heat inactivated samples was 3.2 times the geometric mean value of the uninactivated sera. ELISA IgG antibodies to filamentous hemagglutinin, fimbriae-2, and pertactin were studied, and the values of heat inactivated sera did not differ significantly from the values of the uninactivated sera. Our findings indicate that heat inactivation of sera leads to higher, variable, and false-positive PT and IgG values. SN - 0732-8893 UR - https://www.unboundmedicine.com/medline/citation/9488826/Effect_of_heat_inactivation_of_serum_on_Bordetella_pertussis_antibody_determination_by_enzyme_linked_immunosorbent_assay_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(97)00189-2 DB - PRIME DP - Unbound Medicine ER -